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Lenalidomide acesulfamate: Crystal structure, solid state characterization and dissolution performance EI Scopus SCIE
期刊论文 | 2019 , 1175 , 852-857 | Journal of Molecular Structure
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Abstract :

Lenalidomide (LEN) is a first line drug in the treatment of multiple myeloma, myelodysplastic syndromes, and mantle cell lymphoma. While, this compound holds poor water solubility, leading to unsatisfied bioavailability. To improve the solubility of LEN, a molecular salt with an artificial sweetener, acesulfame (AH), was obtained in this study. The LEN-AH salt was fully characterized by single-crystal and powder X-ray diffraction (XRD), 13C solid state nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC), as well as dynamic vapour sorption (DVS). The crystal structure of LEN-AH clearly reveals the intermolecular proton transfer from NH group of AH to NH2 of LEN. The ionization state of LEN-AH was further confirmed by its feature 13C chemical shifts. The maximum solubility value of LEN was significantly enhanced after salt formation. And the supersaturation state of LEN solution can be maintained at a higher level with the presence of 0.1% PVP-K30. © 2018 Elsevier B.V.

Keyword :

Artificial sweeteners Intermolecular interactions Lenalidomide Myelodysplastic syndromes Single-crystal and powder Solid state characterization Solid-state nuclear magnetic resonance Solubilization

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GB/T 7714 Chen, Xin , Li, Duanxiu , Wang, Jianming et al. Lenalidomide acesulfamate: Crystal structure, solid state characterization and dissolution performance [J]. | Journal of Molecular Structure , 2019 , 1175 : 852-857 .
MLA Chen, Xin et al. "Lenalidomide acesulfamate: Crystal structure, solid state characterization and dissolution performance" . | Journal of Molecular Structure 1175 (2019) : 852-857 .
APA Chen, Xin , Li, Duanxiu , Wang, Jianming , Deng, Zongwu , Zhang, Hailu . Lenalidomide acesulfamate: Crystal structure, solid state characterization and dissolution performance . | Journal of Molecular Structure , 2019 , 1175 , 852-857 .
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miR-603 targeted hexokinase-2 to inhibit the malignancy of ovarian cancer cells. PubMed Scopus SCIE
期刊论文 | 2019 , 661 , 1-9 | Archives of biochemistry and biophysics
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Abstract :

The Warburg effect, characterized by energy production through a high rate of aerobic glycolysis, is a metabolic hallmark of cancer cells. We previously found that ginsenoside 20(S)-Rg3 upregulated miR-603 and impaired the malignancy of ovarian cancer cells by inhibiting the Warburg effect. However, the precise functional role of miR-603 in ovarian cancer progression remains poorly defined. Here, we report that the level of miR-603 in ovarian cancer tissues is significantly lower than that in para-tumor tissues. Overexpression of miR-603 in ovarian cancer cells inhibits the Warburg effect as evidenced by a decrease in glucose consumption, lactate production and hexokinase-2 (HK2) expression, reduces cell proliferation in vitro, and weakens their migration and invasion. Further, miR-603 directly targets HK2 as indicated in a luciferase reporter assay. In contrast to agomiR-NC, agomiR-603 treatment significantly inhibits tumor growth in vivo and the Warburg effect, which is illustrated by a decreased uptake of <sup>18</sup>F-FDG in subcutaneous xenografts and HK2 downregulation. Finally, miR-603 is negatively regulated by DNMT3A-mediated DNA methylation in the promoter region of its precursor gene, suggesting that 20(S)-Rg3 antagonizes DNMT3A-mediated DNA methylation to impair growth, migration and invasion of ovarian cancer cells. In conclusion, miR-603 is a tumor suppressor targeting HK2 in ovarian cancer and its low level may result from DNMT3A-mediated methylation.

Keyword :

Ovarian cancer Warburg effect MicroRNA Methylation

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GB/T 7714 Lu Jiaojiao , Wang Lijie , Chen Wei et al. miR-603 targeted hexokinase-2 to inhibit the malignancy of ovarian cancer cells. [J]. | Archives of biochemistry and biophysics , 2019 , 661 : 1-9 .
MLA Lu Jiaojiao et al. "miR-603 targeted hexokinase-2 to inhibit the malignancy of ovarian cancer cells." . | Archives of biochemistry and biophysics 661 (2019) : 1-9 .
APA Lu Jiaojiao , Wang Lijie , Chen Wei , Wang Yueling , Zhen Shuai , Chen Hong et al. miR-603 targeted hexokinase-2 to inhibit the malignancy of ovarian cancer cells. . | Archives of biochemistry and biophysics , 2019 , 661 , 1-9 .
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Prices, availability and affordability of insulin products: a cross-sectional survey in Shaanxi Province, western China. PubMed Scopus SCIE
期刊论文 | 2019 , 24 (1) , 43-52 | Tropical medicine & international health : TM & IH
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Abstract :

To evaluate price, availability and affordability of insulin products in Shaanxi Province, western China.We used a simplified and adapted WHO/Health Action International method to obtain the availability and prices of insulin products and 5 oral anti-diabetic medicines as comparators in public general hospitals and private retail outlets. In addition, we investigated the price components of 8 selected insulin products by tracing the supply chain.All three kinds of insulin products, prandial, basal and premixed insulin, are 100% available in public hospitals, and have fairly high availability in the private sector (62.5% to 68.8%). The prices of most insulin products were higher than international reference prices in both sectors (ranging from 0.95 times to 2.33 times). All insulin products were unaffordable as they would cost 3.5 to 17.1 days' wage of the lowest-paid government workers in Shaanxi. The manufacturer's markup (selling price), which comprised more than 60% of the final price of all insulin products surveyed, was the largest price component.Although availability of insulin products was high in public general hospitals and private retail pharmacies, their high price made them unaffordable to diabetes patients, especially low-income patients. The government should increase insurance compensation for those who need these life-saving medicines or decrease the cost of insulin products through negotiation with suppliers. This article is protected by copyright. All rights reserved.

Keyword :

price components insulin China affordability availability

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GB/T 7714 Li Zongjie , Feng Qinqin , Kabba John Alimamy et al. Prices, availability and affordability of insulin products: a cross-sectional survey in Shaanxi Province, western China. [J]. | Tropical medicine & international health : TM & IH , 2019 , 24 (1) : 43-52 .
MLA Li Zongjie et al. "Prices, availability and affordability of insulin products: a cross-sectional survey in Shaanxi Province, western China." . | Tropical medicine & international health : TM & IH 24 . 1 (2019) : 43-52 .
APA Li Zongjie , Feng Qinqin , Kabba John Alimamy , Yang Caijun , Chang Jie , Jiang Minghuan et al. Prices, availability and affordability of insulin products: a cross-sectional survey in Shaanxi Province, western China. . | Tropical medicine & international health : TM & IH , 2019 , 24 (1) , 43-52 .
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Adverse drug reaction reporting in institutions across six Chinese provinces: a cross-sectional study. PubMed SCIE
期刊论文 | 2019 , 18 (1) , 59-68 | Expert opinion on drug safety
WoS CC Cited Count: 2
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Abstract :

Although China's adverse drug reaction (ADR) reporting and monitoring has developed rapidly, many challenges remain. This study assessed ADR monitoring and reporting in China and identified monitoring problems.A cross-sectional survey was conducted of ADR reporting institutions in six Chinese provinces in April-December 2014. Questionnaires assessed ADR systems, basic resources, and pharmacovigilance activity.Of 720 questionnaires distributed, the response rate was 81.8%. About 93% (n = 371) of pharmaceutical companies and medical institutions had established ADR monitoring departments/units. Few institutions (26%, n = 104) allocated an ADR budget; 7% (n = 30) had received ADR monitoring funding in the last year (2013). Almost all institutions (99%, n = 555) had computers and 47% (n = 263) had a network database. Many institutions conducted public education about drug safety (49%, n = 283), medicine utilization reviews/quality surveys (28%, n = 158), and medicine consultation services (88%, n = 511). Institutions in eastern, central, and western China differed significantly on implementation of existing regulations and pharmacovigilance activities.The institutions surveyed have established ADR monitoring systems. However, these systems have flaws. Urgent improvements are needed in funding, basic resources, reporting processes, and other pharmacovigilance activities.

Keyword :

Adverse drug reaction reporting hospital cross-sectional study China pharmaceutical manufacturers drugstore pharmacovigilance

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GB/T 7714 Zhang Xuemei , Niu Rui , Feng Bianling et al. Adverse drug reaction reporting in institutions across six Chinese provinces: a cross-sectional study. [J]. | Expert opinion on drug safety , 2019 , 18 (1) : 59-68 .
MLA Zhang Xuemei et al. "Adverse drug reaction reporting in institutions across six Chinese provinces: a cross-sectional study." . | Expert opinion on drug safety 18 . 1 (2019) : 59-68 .
APA Zhang Xuemei , Niu Rui , Feng Bianling , Guo Jiadong , Liu Ying , Liu Xinyu . Adverse drug reaction reporting in institutions across six Chinese provinces: a cross-sectional study. . | Expert opinion on drug safety , 2019 , 18 (1) , 59-68 .
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Development and evaluation of hollow mesoporous silica microspheres bearing on enhanced oral delivery of curcumin. PubMed SCIE
期刊论文 | 2019 , 45 (2) , 273-281 | Drug development and industrial pharmacy
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Abstract :

The aim of this work is to develop curcumin-loaded hollow mesoporous silica microspheres (HMSMs@curcumin) to improve the poor oral bioavailability of curcumin. Hollow mesoporous silica microspheres (HMSMs) were synthesized in facile route using a hard template. HMSMs and HMSMs@curcumin were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), nitrogen adsorption/desorption measurements, differential scanning calorimetry (DSC), Fourier transform infrared (FTIR) and X-ray diffraction (XRD). In addition, to demonstrate the potential application of the HMSMs@curcumin, cytotoxicity, in vitro release behavior and in vivo pharmacokinetics of curcumin loaded in these HMSMs were investigated by using of Caco-2 cells and Sprague-Dawley (SD) rats, respectively. These mono-dispersed HMSMs exhibited high drug loading ratio and encapsulation efficiency due to the mesoporous shell and hollow core. The excellent characteristics of HMSMs such as mono-dispersed morphology, smooth surface, uniform, ordered and size-narrowing mesopores resulted in a good in vitro release profile of curcumin from HMSMs@curcumin. Moreover, an impressive improvement in the oral absorption of curcumin and prolonged systemic circulation time were achieved in the in vivo animal studies. In addition, the good biocompatibility of developed HMSMs with Caco-2 cells was confirmed based on the in vitro cytotoxicity assay. In conclusion, this system demonstrated a great potential for efficiently delivery of curcumin in vitro and in vivo, suggesting a good prospect for its application in clinic for therapeutic drug delivery in future.

Keyword :

Cytotoxicity Hollow mesoporous silica microspheres (HMSMs) Curcumin In vivo drug delivery In vitro drug release

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GB/T 7714 Gao Yang , Ding Shujiang , Huang Xiaoyan et al. Development and evaluation of hollow mesoporous silica microspheres bearing on enhanced oral delivery of curcumin. [J]. | Drug development and industrial pharmacy , 2019 , 45 (2) : 273-281 .
MLA Gao Yang et al. "Development and evaluation of hollow mesoporous silica microspheres bearing on enhanced oral delivery of curcumin." . | Drug development and industrial pharmacy 45 . 2 (2019) : 273-281 .
APA Gao Yang , Ding Shujiang , Huang Xiaoyan , Fan Zhaoyang , Sun Jianmei , Hai Yang et al. Development and evaluation of hollow mesoporous silica microspheres bearing on enhanced oral delivery of curcumin. . | Drug development and industrial pharmacy , 2019 , 45 (2) , 273-281 .
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Dual-mixed/CMC model for screening target components from traditional Chinese medicines simultaneously acting on EGFR & FGFR4 receptors. EI PubMed Scopus SCIE
期刊论文 | 2019 , 192 , 248-254 | Talanta
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Abstract :

Radix Salviae Miltiorrhiae (also known as DanShen (DS) in China), a popular herbal drug in traditional Chinese medicine (TCM) for promoting blood circulation and treating blood stasis, has been reported to possess potential anti-tumor effects. The aim of the study was to develop an effective and practical method for screening and identifying bioactive compounds from Radix Salviae Miltiorrhiae. In this work, the epidermal growth factor receptor (EGFR) and fibroblast growth factor receptors 4 (FGFR4) dual-mixed/cell membrane chromatography (CMC) coupled with high performance liquid chromatography-electrospray ionization-ion trap-time of flight-multistage mass spectrum (HPLC-ESI-IT-TOF-MSn) was established and successfully used to identify the active components from Radix Salviae Miltiorrhiae. Salvianolic acid C (SAC), tanshinone I (Tan-I), tanshinone IIA (Tan-IIA), and cryptotanshinone (C-Tan) were identified as bioactive components with EGFR and FGFR4 activities. MTT and kinase assay were performed to investigate inhibitory effects of these compounds against EGFR and FGFR4 cells growth in vitro. Both cell viability and kinase activity showed that cryptotanshinone acting on EGFR receptor and tanshinone IIA acting on FGFR4 receptor. In conclusion, the EGFR & FGFR4 dual-mixed/CMC can simultaneously screen the bioactive components from TCMs that act on both EGFR and FGFR4 receptors, which significantly improve the efficiency of specific bioactive components identification from a complex system.

Keyword :

Epidermal growth factor receptor HPLC-ESI-IT-TOF-MSn Radix Salviae Miltiorrhiaeis Dual-mixed/cell membrane chromatography Fibroblast growth factor receptors 4

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GB/T 7714 Fu Jia , Lv Yanni , Jia Qianqian et al. Dual-mixed/CMC model for screening target components from traditional Chinese medicines simultaneously acting on EGFR & FGFR4 receptors. [J]. | Talanta , 2019 , 192 : 248-254 .
MLA Fu Jia et al. "Dual-mixed/CMC model for screening target components from traditional Chinese medicines simultaneously acting on EGFR & FGFR4 receptors." . | Talanta 192 (2019) : 248-254 .
APA Fu Jia , Lv Yanni , Jia Qianqian , Lin Yuanyuan , Han Shengli . Dual-mixed/CMC model for screening target components from traditional Chinese medicines simultaneously acting on EGFR & FGFR4 receptors. . | Talanta , 2019 , 192 , 248-254 .
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Silencing of LncRNA steroid receptor RNA activator attenuates polycystic ovary syndrome in mice Scopus SCIE PubMed
期刊论文 | 2019 , 157 , 48-56 | Biochimie
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Abstract :

© 2018 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM) Background: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age and has a prevalence of 1 in 15 women worldwide. This study aims to investigate the role of lncRNA SRA in the pathological processes of polycystic ovary syndrome (PCOS). Methods: Twenty five-day old female C57BL/6 mice received subcutaneous injection of 60 mg/kg dehydroepiandrosterone for 20 days to induce PCOS. Lentivirus containing lncRNA SRA-specific shRNA was subcapsularly injected into the ovaries of PCOS mice. Granulosa cell was primary cultured to explore the mechanism of DHEA-induced inflammatory responses. H&E staining was used to examine the histological changes of ovaries. ELISA was used to assess serum insulin level and proinflammatory cytokines and angiogenetic factors contents in ovary tissue. The expression levels of LncRNA SRA and proteins involved in the NF-κB signaling pathway were detected through Quantitative real-time PCR and Western blot. The nuclear translocation of NF-κB was observed by immunofluorescence and the activity of NF-κB-DNA binding was detected using EMSA. Results: Silencing of lncRNA SRA changed insulin release, attenuated ovary injury and reduced the production of angiogenetic factors in the PCOS mice. In addition, shRNA targeting lncRNA SRA inhibited DHEA-induced pro-inflammatory cytokines production and NF-κB nuclear translocation in the ovary of PCOS mice and primary granulosa cells. Conclusion: Silencing of lncRNA Steroid Receptor RNA Activator (SRA) attenuates polycystic ovary syndrome (PCOS) in mice. LncRNA SRA plays important roles in the development of PCOS.

Keyword :

Angiogenesis Granulosa cell Inflammation LncRNA SRA PCOS

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GB/T 7714 Li, Yan , Zhao, Wanqiu , Wang, Haixu et al. Silencing of LncRNA steroid receptor RNA activator attenuates polycystic ovary syndrome in mice [J]. | Biochimie , 2019 , 157 : 48-56 .
MLA Li, Yan et al. "Silencing of LncRNA steroid receptor RNA activator attenuates polycystic ovary syndrome in mice" . | Biochimie 157 (2019) : 48-56 .
APA Li, Yan , Zhao, Wanqiu , Wang, Haixu , Chen, Chen , Zhou, Dongmei , Li, Shengnan et al. Silencing of LncRNA steroid receptor RNA activator attenuates polycystic ovary syndrome in mice . | Biochimie , 2019 , 157 , 48-56 .
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Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration EI PubMed SCIE
期刊论文 | 2019 , 96 , 841-849 | Materials Science and Engineering C
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Bone-marrow-derived mesenchymal stem cells possess great potential for tissue engineering and regenerative medicine. In the work, an injectable BMSCs-laden hydrogel system was formed by enzyme-catalyzed crosslinking of hyaluronic acid-tyramine and chondroitin sulfate-tyramine in the presence of hydrogen peroxide and horseradish peroxidase, which was used as a 3D scaffold to explore the behavior of the mesenchymal stem cells. Afterward, the gelation rate, mechanical properties, as well as the degradation process of the scaffold were well characterized and optimized. Furthermore, bone morphogenetic protein-2 was encapsulated in the scaffold, which was used to improve the osteogenic properties. The results illustrated that such a BMSCs-laden hydrogel not only offered a proper microenvironment for the adhesion, proliferation and differentiation of mesenchymal stem cells in vitro, but also promoted bone regeneration in vivo. Therefore, this injectable BMSCs-laden hydrogel may serve as an efficient 3D scaffold for bone repair and regeneration. © 2018 Elsevier B.V.

Keyword :

Bone marrow-derived mesenchymal stem cells Bone morphogenetic protein-2 Chondroitin sulfates Degradation process Horse-radish peroxidase Injectable hydrogels Mesenchymal stem cell Osteogenic differentiation

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GB/T 7714 Zhang, Yajie , Chen, Hong , Zhang, Tingting et al. Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration [J]. | Materials Science and Engineering C , 2019 , 96 : 841-849 .
MLA Zhang, Yajie et al. "Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration" . | Materials Science and Engineering C 96 (2019) : 841-849 .
APA Zhang, Yajie , Chen, Hong , Zhang, Tingting , Zan, Yue , Ni, Tianyu , Cao, Yi et al. Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration . | Materials Science and Engineering C , 2019 , 96 , 841-849 .
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The quality of spontaneous adverse drug reaction reports from the pharmacovigilance centre in western China SCIE PubMed
期刊论文 | 2019 , 18 (1) , 51-58 | EXPERT OPINION ON DRUG SAFETY
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Background: High-quality adverse drug reaction (ADR) reports are essential for conducting drug safety monitoring in pharmacovigilance. The study aim was to assess the current quality of ADR reports in western China, and to identify problems with ADR report quality. Research design and methods: A sample of 1139 reports received by the Shaanxi ADR Monitoring Center from January 2015 to December 2017 was selected. ADR report quality was evaluated using an ADR report quality evaluation system. Results: None of the reports were rated as excellent and 1.40% (n = 16) as good. Report quality was better for new and serious reports than for general reports. Medical institutions generated higher quality reports than pharmaceutical manufacturers. Nurses generated higher quality reports than doctors, pharmacists, and other professionals. Reporters of different occupations showed significant differences in the quality of the indicators Reporting time limit, Intervention ADR time, ADR termination time, ADR intervention measures, Original disease, and Cause of medication (P = 0.000). Conclusions: The ADR data quality was poor in western China, and of lower quality than reported data from previous research in other regions. Improvements in the quality and availability of ADR reports are urgently needed.

Keyword :

Spontaneous reporting quality assessment adverse drug reactions drug safety pharmacovigilance

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GB/T 7714 Niu, Rui , Xiang, Yufang , Wu, Tingting et al. The quality of spontaneous adverse drug reaction reports from the pharmacovigilance centre in western China [J]. | EXPERT OPINION ON DRUG SAFETY , 2019 , 18 (1) : 51-58 .
MLA Niu, Rui et al. "The quality of spontaneous adverse drug reaction reports from the pharmacovigilance centre in western China" . | EXPERT OPINION ON DRUG SAFETY 18 . 1 (2019) : 51-58 .
APA Niu, Rui , Xiang, Yufang , Wu, Tingting , Zhang, Zhi , Chen, Yue , Feng, Bianling . The quality of spontaneous adverse drug reaction reports from the pharmacovigilance centre in western China . | EXPERT OPINION ON DRUG SAFETY , 2019 , 18 (1) , 51-58 .
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Combined magnetic porous molecularly imprinted polymers and deep eutectic solvents for efficient and selective extraction of aristolochic acid I and II from rat urine EI PubMed SCIE
期刊论文 | 2019 , 97 , 650-657 | Materials Science and Engineering C
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Aristolochia and related plants contain nephrotoxins and mutagens in the form of aristolochic acids (AAs). However, there is still lack of a fast and specific method for monitoring AAs in biological samples. Herein, we synthesized a hybrid magnetic mesoporous carbon-molecularly imprinted polymers (MMC@MIPs) as a novel magnetic solid-phase extraction (MSPE) adsorbent for selective recognition of aristolochic acid I and II from rat urine samples. The choline chloride/glycol-based deep eutectic solvent (DES) and indomethacin were used as the eluent and dummy template molecule accordingly. The morphology, structure property and surface groups of the prepared materials were investigated in sequence, and the optimum conditions of the MMC@MIPs-MSPE procedure were also optimized well. Results showed that the proposed method had a relatively satisfactory recovery (86.7–94.3%), with low standard deviation (<4.85%) and acceptable correlation coefficients (0.991–0.996). Overall, this work not only provides an inexpensive and eco-friendly method to fabricate MMC@MIPs, but also develops a highly promising approach for the detection of aristolochic acid I and II in biological samples. © 2018 Elsevier B.V.

Keyword :

Aristolochic acid Deep eutectic solvents Magnetic mesoporous carbons Magnetic solid-phase extractions Molecular imprinted polymers

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GB/T 7714 Ge, Yan-Hui , Shu, Hua , Xu, Xin-Ya et al. Combined magnetic porous molecularly imprinted polymers and deep eutectic solvents for efficient and selective extraction of aristolochic acid I and II from rat urine [J]. | Materials Science and Engineering C , 2019 , 97 : 650-657 .
MLA Ge, Yan-Hui et al. "Combined magnetic porous molecularly imprinted polymers and deep eutectic solvents for efficient and selective extraction of aristolochic acid I and II from rat urine" . | Materials Science and Engineering C 97 (2019) : 650-657 .
APA Ge, Yan-Hui , Shu, Hua , Xu, Xin-Ya , Guo, Peng-Qi , Liu, Rui-Lin , Luo, Zhi-Min et al. Combined magnetic porous molecularly imprinted polymers and deep eutectic solvents for efficient and selective extraction of aristolochic acid I and II from rat urine . | Materials Science and Engineering C , 2019 , 97 , 650-657 .
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