To　measure　the　situation　of　the　non-prescription　sale　of　antibiotics　and　the　service　quality　of　community　pharmacies　in　Guangzhou,　China.A　simulated　client　method　was　conducted　to　estimate　the　non-prescription　sale　of　antibiotics　and　service　quality　based　on　scenarios　about　adult　acute　upper　respiratory　tract　infection　in　2019.　A　total　of　595　community　pharmacies　from　11　districts　were　investigated　in　Guangzhou,　China.　We　used　binary　logistic　regression　to　evaluate　the　factors　associated　with　the　non-prescription　sale　of　antibiotics.The　proportion　of　non-prescription　dispensing　of　antibiotics　was　63.1%　in　Guangzhou,　China,　with　a　higher　incidence　of　antibiotic　dispensing　without　prescription　in　outer　districts　(69.3%).　Cephalosporin　(44.1%)　and　Amoxicillin　(39.0%)　were　sold　more　often　than　other　antibiotics.　Chain　pharmacies　had　better　performance　on　the　prescription　sale　of　antibiotics　and　service　quality.　Traditional　Chinese　medicine　was　commonly　recommended　by　pharmacy　staff.Since　the　non-prescription　sale　of　antibiotics　is　prevalent　in　Guangzhou,　effective　solutions　should　be　determined.　Strengthened　public　awareness　and　regulatory　system　innovation　are　needed.