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A set of autosomal multiple InDel markers for forensic application and population genetic analysis in the Chinese Xinjiang Hui group SSCI SCIE PubMed Scopus
期刊论文 | 2018 , 35 , 1-8 | FORENSIC SCIENCE INTERNATIONAL-GENETICS
WoS CC Cited Count: 1 SCOPUS Cited Count: 2
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Abstract :

In recent years, insertion/deletion (InDel) markers have become a promising and useful supporting tool in forensic identification cases and biogeographic research field. In this study, 30 InDel loci were explored to reveal the genetic diversifies and genetic relationships between Chinese Xinjiang Hui group and the 25 previously reported populations using various biostatistics methods such as forensic statistical parameter analysis, phylogenetic reconstruction, mull-dimensional scaling, principal component analysis, and STRUCTURE analysis. No deviations from Hardy-Weinberg equilibrium tests were found at all 30 loci in the Chinese Xinjiang Hui group. The observed heterozygosity and expected heterozygosity ranged from 0.1971 (HLD118) to 0.5092 (HLD92), 0.2222 (HLD118) to 0.5000 (HLD6), respectively. The cumulative probability of exclusion and combined power of discrimination were 0.988849 and 0.99999999999378, respectively, which indicated that these 30 loci could be qualified for personal identification and used as complementary genetic markers for paternity tests in forensic cases. The results of present research based on the different methods of population genetic analysis revealed that the Chinese Xinjiang Hui group had close relationships with most Chinese groups, especially Han populations. In spite of this, for a better understanding of genetic background of the Chinese Xinjiang Hui group, more molecular genetic markers such as ancestry informative markers, single nucleotide polymorphisms (SNPs), and copy number variations will be conducted in future studies.

Keyword :

PCA Phylogenetic reconstruction Xinjiang Hui group InDel Population genetic analysis Human identification

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GB/T 7714 Xie, Tong , Guo, Yuxin , Chen, Ling et al. A set of autosomal multiple InDel markers for forensic application and population genetic analysis in the Chinese Xinjiang Hui group [J]. | FORENSIC SCIENCE INTERNATIONAL-GENETICS , 2018 , 35 : 1-8 .
MLA Xie, Tong et al. "A set of autosomal multiple InDel markers for forensic application and population genetic analysis in the Chinese Xinjiang Hui group" . | FORENSIC SCIENCE INTERNATIONAL-GENETICS 35 (2018) : 1-8 .
APA Xie, Tong , Guo, Yuxin , Chen, Ling , Fang, Yating , Tai, Yunchun , Zhou, Yongsong et al. A set of autosomal multiple InDel markers for forensic application and population genetic analysis in the Chinese Xinjiang Hui group . | FORENSIC SCIENCE INTERNATIONAL-GENETICS , 2018 , 35 , 1-8 .
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Genetic structure and polymorphism analysis of Xinjiang Hui ethnic minority based on 21 STRs SCIE PubMed Scopus
期刊论文 | 2018 , 45 (2) , 99-108 | MOLECULAR BIOLOGY REPORTS
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Abstract :

In the present study, we calculated the allelic frequencies and forensic descriptive parameters of Hui ethnic minority on the basis of 21 short tandem repeat (STR) loci aiming at understanding population structure better and enriching population genetic database. Bloodstain samples of 506 unrelated healthy Hui individuals in Xinjiang Uygur Autonomous Region were collected. Altogether 268 alleles were observed and the allelic frequencies ranged from 0.0010 to 0.5306. The combined power of discrimination and the cumulative probability of exclusion of the 21 STR loci in Hui ethnic minority were 0.9999999999999999999999998697 and 0.9999999968, respectively. Population data obtained manifested that the panel of 21 STR loci could provide robust genetic information for individual identification and paternity testing involved in forensic applications for Huis of Xinjiang Region. Furthermore, the present results of interpopulation differentiations, phylogenetic trees and principal component analysis which were conducted based on the overlapping 16 STR loci revealed that Hui group was genetically close to Xibe ethnic group and Han populations from different regions.

Keyword :

GlobalFiler Hui ethnic minority Population differentiation STR

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GB/T 7714 Lan, Qiong , Chen, Jiangang , Guo, Yuxin et al. Genetic structure and polymorphism analysis of Xinjiang Hui ethnic minority based on 21 STRs [J]. | MOLECULAR BIOLOGY REPORTS , 2018 , 45 (2) : 99-108 .
MLA Lan, Qiong et al. "Genetic structure and polymorphism analysis of Xinjiang Hui ethnic minority based on 21 STRs" . | MOLECULAR BIOLOGY REPORTS 45 . 2 (2018) : 99-108 .
APA Lan, Qiong , Chen, Jiangang , Guo, Yuxin , Xie, Tong , Fang, Yating , Jin, Xiaoye et al. Genetic structure and polymorphism analysis of Xinjiang Hui ethnic minority based on 21 STRs . | MOLECULAR BIOLOGY REPORTS , 2018 , 45 (2) , 99-108 .
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Forensic efficiency estimate and phylogenetic analysis for Chinese Kyrgyz ethnic group revealed by a panel of 21 short tandem repeats SCIE PubMed Scopus
期刊论文 | 2018 , 5 (6) | ROYAL SOCIETY OPEN SCIENCE
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Short tandem repeats (STRs) with a high level of polymorphisms and convenient detection method play an indispensable role in human population and forensic genetics. Recently, we detected the 21 autosomal non-combined DNA index system (non-CODIS) STR loci in a Kyrgyz ethnic group, calculated their forensic parameters and analysed its genetic relationships with reference populations from China. In total, 168 alleles were observed at 21 non-CODIS STRs with corresponding allelic frequencies from 0.0016 to 0.4788. No significant deviations at these STRs were observed from the Hardy-Weinberg equilibrium. The values of cumulative power of discrimination and probability of exclusion for all the 21 non-CODIS STRs were 0.99999999999999999998835 and 0.9999994002, respectively. Furthermore, the analyses of phylogenetic trees, genetic distances and interpopulation differentiations demonstrated that the Kyrgyz group had relatively close genetic relationships with the Uygur and Kazak groups. These 21 non-CODIS STRs were characterized by high genetic diversities in the Kyrgyz group and could be applied as a robust tool for individual identification and kinship testing in forensic sciences.

Keyword :

Chinese Kyrgyz non-CODIS STRs forensic population genetics

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GB/T 7714 Guo, Yuxin , Chen, Chong , Xie, Tong et al. Forensic efficiency estimate and phylogenetic analysis for Chinese Kyrgyz ethnic group revealed by a panel of 21 short tandem repeats [J]. | ROYAL SOCIETY OPEN SCIENCE , 2018 , 5 (6) .
MLA Guo, Yuxin et al. "Forensic efficiency estimate and phylogenetic analysis for Chinese Kyrgyz ethnic group revealed by a panel of 21 short tandem repeats" . | ROYAL SOCIETY OPEN SCIENCE 5 . 6 (2018) .
APA Guo, Yuxin , Chen, Chong , Xie, Tong , Cui, Wei , Meng, Haotian , Jin, Xiaoye et al. Forensic efficiency estimate and phylogenetic analysis for Chinese Kyrgyz ethnic group revealed by a panel of 21 short tandem repeats . | ROYAL SOCIETY OPEN SCIENCE , 2018 , 5 (6) .
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Autosomal DIPs for population genetic structure and differentiation analyses of Chinese Xinjiang Kyrgyz ethnic group SCIE PubMed Scopus
期刊论文 | 2018 , 8 | SCIENTIFIC REPORTS
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In recent years, deletion and insertion polymorphisms (DIPs) were treated as a novel complementary tool with huge potential for forensic applications. In this study, we utilized 30 DIP loci to make a comprehensive research of allele frequency distribution and compute forensic parameters to evaluate the efficiency of forensic applications in the 295 unrelated healthy individuals of Kyrgyz group, and in addition, infer the genetic relationships between Kyrgyz group and 24 other previously studied groups. No significant departures from Hardy-Weinberg equilibrium and linkage disequilibrium were observed at these 30 DIP loci. The combined power of discrimination and the combined probability of exclusion for all 30 DIP loci in Kyrgyz group were 0.9999999999989 and 0.9939, respectively. Furthermore, the results of the interpopulation differentiations, phylogenetic reconstruction, population genetic structure and principal component analyses suggested that Kyrgyz group had relatively close genetic relationships with Kazakh and Uygur groups. However, it was also important to stress that 15 loci were selected out from these 30 DIP loci using the method of selecting ancestry markers, which could be utilized for further ancestry inference study relatively.

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GB/T 7714 Guo, Yuxin , Chen, Chong , Jin, Xiaoye et al. Autosomal DIPs for population genetic structure and differentiation analyses of Chinese Xinjiang Kyrgyz ethnic group [J]. | SCIENTIFIC REPORTS , 2018 , 8 .
MLA Guo, Yuxin et al. "Autosomal DIPs for population genetic structure and differentiation analyses of Chinese Xinjiang Kyrgyz ethnic group" . | SCIENTIFIC REPORTS 8 (2018) .
APA Guo, Yuxin , Chen, Chong , Jin, Xiaoye , Cui, Wei , Wei, Yuanyuan , Wang, Hongdan et al. Autosomal DIPs for population genetic structure and differentiation analyses of Chinese Xinjiang Kyrgyz ethnic group . | SCIENTIFIC REPORTS , 2018 , 8 .
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Mutability analysis towards 21 STR loci included in the AGCU 21+1 kit in Chinese Han population SCIE PubMed Scopus
期刊论文 | 2018 , 132 (5) , 1287-1291 | INTERNATIONAL JOURNAL OF LEGAL MEDICINE
SCOPUS Cited Count: 1
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To further illustrate the mutation traits of 21 autosomal STR loci involved in the newly developed AGCU 21 + 1 kit, randomly selected 485 trios were focused on. We have previously confirmed the biological father-mother-child relationships of these trios. Then matters of mutation rates, steps, and origins of the 21 STR loci were statically analyzed. Results showed 35 mutation events occurred at 16 STR loci of the panel during 20,370 meiosis, and the locus-specific mutation rates ranged from 0.000 to 1.134% (D3S4529), with an overall mutation rate of 1.72 x 10(-3) (95%CI, 1.20-2.39 x 10(-3)). Mutation origins exhibited slight discrepancy between male and female germlines, 18 of the 35 mutation events were verified to be contributed by fathers and 13 by mothers, with origins of the remaining 4 events hardly to be assigned. Furthermore, 77.14% of the mutations were one-step mutation. Currently, portraits on mutability of the STR loci involved in the AGCU 21 + 1 panel were barely reported. In view of this, we conclude our data will enrich the mutation information of the 21 STR loci and provide valuable suggestions in calculating likelihood ratios for mutation-involved parental testing cases.

Keyword :

Mutation rate Han population AGCU 21+1 Short tandem repeats

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GB/T 7714 Lan, Qiong , Wang, Hongdan , Shen, Chunmei et al. Mutability analysis towards 21 STR loci included in the AGCU 21+1 kit in Chinese Han population [J]. | INTERNATIONAL JOURNAL OF LEGAL MEDICINE , 2018 , 132 (5) : 1287-1291 .
MLA Lan, Qiong et al. "Mutability analysis towards 21 STR loci included in the AGCU 21+1 kit in Chinese Han population" . | INTERNATIONAL JOURNAL OF LEGAL MEDICINE 132 . 5 (2018) : 1287-1291 .
APA Lan, Qiong , Wang, Hongdan , Shen, Chunmei , Guo, Yuxin , Yin, Caiyong , Xie, Tong et al. Mutability analysis towards 21 STR loci included in the AGCU 21+1 kit in Chinese Han population . | INTERNATIONAL JOURNAL OF LEGAL MEDICINE , 2018 , 132 (5) , 1287-1291 .
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Forensic molecular genetic diversity analysis of Chinese Hui ethnic group based on a novel STR panel SCIE PubMed Scopus
期刊论文 | 2018 , 132 (5) , 1297-1299 | INTERNATIONAL JOURNAL OF LEGAL MEDICINE
WoS CC Cited Count: 1 SCOPUS Cited Count: 1
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In present study, the genetic polymorphisms of 22 autosomal short tandem repeat (STR) loci were analyzed in 496 unrelated Chinese Xinjiang Hui individuals. These autosomal STR loci were multiplex amplified and genotyped based on a novel STR panel. There were 246 observed alleles with the allele frequencies ranging from 0.0010 to 0.3609. All polymorphic information content values were higher than 0.7. The combined power of discrimination and the combined probability of exclusion were 0.999999999999999999999999999426766 and 0.999999999860491, respectively. Based on analysis of molecular variance method, genetic differentiation analysis between the Xinjiang Hui and other reported groups were conducted at these 22 loci. The results indicated that there were no significant differences in statistics between Hui group and Northern Han group (including Han groups from Hebei, Henan, Shaanxi provinces), and significant deviations with Southern Han group (including those from Guangdong, Guangxi provinces) at 7 loci, and Uygur group at 10 loci. To sum up, these 22 autosomal STR loci were high genetic polymorphic in Xinjiang Hui group.

Keyword :

Forensic genetics Hui group STR Genetic polymorphism

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GB/T 7714 Fang, Yating , Guo, Yuxin , Xie, Tong et al. Forensic molecular genetic diversity analysis of Chinese Hui ethnic group based on a novel STR panel [J]. | INTERNATIONAL JOURNAL OF LEGAL MEDICINE , 2018 , 132 (5) : 1297-1299 .
MLA Fang, Yating et al. "Forensic molecular genetic diversity analysis of Chinese Hui ethnic group based on a novel STR panel" . | INTERNATIONAL JOURNAL OF LEGAL MEDICINE 132 . 5 (2018) : 1297-1299 .
APA Fang, Yating , Guo, Yuxin , Xie, Tong , Jin, Xiaoye , Lan, Qiong , Zhou, Yongsong et al. Forensic molecular genetic diversity analysis of Chinese Hui ethnic group based on a novel STR panel . | INTERNATIONAL JOURNAL OF LEGAL MEDICINE , 2018 , 132 (5) , 1297-1299 .
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Doxorubicin conjugated carbon dots as a drug delivery system for human breast cancer therapy SCIE PubMed Scopus
期刊论文 | 2018 , 51 (5) | CELL PROLIFERATION
WoS CC Cited Count: 1 SCOPUS Cited Count: 2
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ObjectivesCarbon dots (CDs) are one of the most promising carbon-based materials in bioimaging and drug/gene delivery applications. In this study, we have attempted to study the drug carrying capacity of highly fluorescent CDs for delivery of doxorubicin (DOX) and investigate the therapeutic activity of the CDs-DOX drug delivery system. Materials and methodsCarbon dots were synthesized by means of a hydrothermal approach with mixing citric acid and ethylenediamine. The properties of CDs were characterized in respects of spectral property, zeta potential, particle morphology and chemical composition. The drug loading efficiency (DLE) and release profile of CDs-DOX were determined by a fluorescence spectrophotometer. We investigated the cellular toxicity and pharmaceutical activity of CDs and CDs-DOX in L929 cells and MCF-7 cells by the CCK-8 assay. We also studied the cellular uptake of CDs-DOX with the methods of confocal microscopy and flow cytometry. In addition, the effect of CDs-DOX on cell apoptosis was assessed by flow cytometry. ResultsThe obtained CDs possessed good biocompatibility and showed a potential capacity of promoting proliferation. DOX was successfully conjugated to CDs through electrostatic interaction, and the results of the DLE and loading content (DLC) suggested a relatively high drug loading capacity of CDs. Compared with free DOX, the CDs-DOX complex had a higher cellular uptake and better anti-tumour efficacy on MCF-7 cells. ConclusionsThe results of this study indicated that the CDs-DOX drug delivery system had a potential value in cancer chemotherapeutic application.

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GB/T 7714 Kong, Tingting , Hao, Liying , Wei, Yuanyuan et al. Doxorubicin conjugated carbon dots as a drug delivery system for human breast cancer therapy [J]. | CELL PROLIFERATION , 2018 , 51 (5) .
MLA Kong, Tingting et al. "Doxorubicin conjugated carbon dots as a drug delivery system for human breast cancer therapy" . | CELL PROLIFERATION 51 . 5 (2018) .
APA Kong, Tingting , Hao, Liying , Wei, Yuanyuan , Cai, Xiaoxiao , Zhu, Bofeng . Doxorubicin conjugated carbon dots as a drug delivery system for human breast cancer therapy . | CELL PROLIFERATION , 2018 , 51 (5) .
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TNFα-308G/A位点基因多态性与糖尿病性牙周炎易感性的相关性研究
期刊论文 | 2018 , (8) , 440-445 | 牙体牙髓牙周病学杂志
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目的:探讨肿瘤坏死因子-α(TNFα)基因多态性与糖尿病性牙周炎(DP)易感性的相关性.方法:电子检索MEDLINE、Web of Science、CNKI、万方以及中国生物医学文摘数据库自建库至2016-04收录的所有文献,从中收集TNFα-(308G/A)位点基因多态性与DP易感性的相关研究并进行meta分析.同时采用病例对照研究进行验证:分别收集慢性牙周炎(CP)患者(60例)、健康对照者(N)(56例)、2型糖尿病(T2DM)患者(58例)、DP患者(60例)的血液样本并采用DNA测序法检测其TNFα-(308G/A)位点的基因型.随后采用χ2检验分析基因型和等位基因频率在各组间分布的差异,并应用非条件Logistic回归分析计算风险比(OR)及95%可信区间(95%CI),以评估各基因型与DP发病风险的相关性.结果:meta分析结果显示,TNFα-(308G/A)位点多态性可能是汉族人群DP易感性的相关因素.病例对照研究结果显示,TNFα-(308G/A)位点的3种基因型(GG、GA、AA)及等位基因(G、A)频率在各组间的分布均有统计学差异(P<0.05);携带A等位基因的人群患DP的风险明显增加(OR=5.074,95%CI:1.857~13.868,P=0.002),而且携带GA基因型的人群患DP的风险显著高于携带GG基因型的人群(OR=5.637,95%CI:1.949~16.301,P=0.001).结论:汉族人群中TNFα-(308G/A)位点基因多态性可能是DP易感性的相关因素.

Keyword :

基因多态性 TNFα 糖尿病性牙周炎

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GB/T 7714 王欣欣 , 辛欣 , 李晔 et al. TNFα-308G/A位点基因多态性与糖尿病性牙周炎易感性的相关性研究 [J]. | 牙体牙髓牙周病学杂志 , 2018 , (8) : 440-445 .
MLA 王欣欣 et al. "TNFα-308G/A位点基因多态性与糖尿病性牙周炎易感性的相关性研究" . | 牙体牙髓牙周病学杂志 8 (2018) : 440-445 .
APA 王欣欣 , 辛欣 , 李晔 , 孟昊天 , 朱波峰 , 李昂 et al. TNFα-308G/A位点基因多态性与糖尿病性牙周炎易感性的相关性研究 . | 牙体牙髓牙周病学杂志 , 2018 , (8) , 440-445 .
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Genetic polymorphism analysis of mitochondrial DNA from Chinese Xinjiang Kazak ethnic group by a novel mitochondrial DNA genotyping panel. PubMed
期刊论文 | 2018 | Molecular biology reports
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Genetic polymorphism analysis of 60 mitochondrial DNA (mtDNA) loci in Chinese Xinjiang Kazak group was conducted in this study. Blood samples from 141 unrelated healthy volunteers were randomly collected from Chinese Kazak ethnic group in Ili, Xinjiang Uygur Autonomous region. Among these mtDNA loci, single nucleotide transition was the most commonly observed variant (87.93%). A total of 25 haplogroups and 79 haplotypes were found in Kazak group, and Haplogroup D4 was the most common haplogroup (21.28%). Among the entire 79 haplotypes, 53 of them were observed for only once, 14 for twice. The haplotype diversity was 0.978 ± 0.005, and the nucleotide diversity was 0.17449. The detection of (CA)n and 9-bp deletion polymorphisms could improve the discrimination power of the mtDNA genetic marker. Moreover, Xinjiang Kazak group was compared with other previously reported groups to infer its genetic background. The present results revealed that Xinjiang Kazak ethnic group was genetically closer related to Xinjiang Uygur, Xinjiang Uzbek and Xinjiang Han populations. Meanwhile, our results also indicated the potential closer genetic relationships among Xinjiang Kazak group with Altaian Kazak as well as Xinjiang Xibe group. In conclusion, this novel mtDNA panel could be effectively utilized for forensic applications. Additionally, to further reveal the genetic background of Chinese Kazak group, more relevant populations and genetic markers should be incorporated in our future study.

Keyword :

Kazak Haplogroup SNP mtDNA

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GB/T 7714 Xie Tong , Hu Li , Guo Yu-Xin et al. Genetic polymorphism analysis of mitochondrial DNA from Chinese Xinjiang Kazak ethnic group by a novel mitochondrial DNA genotyping panel. [J]. | Molecular biology reports , 2018 .
MLA Xie Tong et al. "Genetic polymorphism analysis of mitochondrial DNA from Chinese Xinjiang Kazak ethnic group by a novel mitochondrial DNA genotyping panel." . | Molecular biology reports (2018) .
APA Xie Tong , Hu Li , Guo Yu-Xin , Li Yu-Chun , Chen Feng , Zhu Bo-Feng . Genetic polymorphism analysis of mitochondrial DNA from Chinese Xinjiang Kazak ethnic group by a novel mitochondrial DNA genotyping panel. . | Molecular biology reports , 2018 .
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Genetic diversity and haplotypic structure of Chinese Kazak ethnic group revealed by 19 STRs on the X chromosome SCIE PubMed Scopus
期刊论文 | 2017 , 600 , 64-69 | GENE | IF: 2.498
WoS CC Cited Count: 8 SCOPUS Cited Count: 9
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X-chromosomal short tandem repeats (X-STRs) have been widely used in forensic practices involving complicated ties of kinship over the past years, and also play an increasingly important role in population genetics. To study the genetic polymorphisms of 19 STR loci on X chromosome in Chinese Kazak ethnic group, we investigated the allelic and haplotypic frequencies of the 19 loci in 300(149 males and 151 females) unrelated healthy individuals from Ili Kazak Autonomous Prefecture, Xinjiang Uigur Autonomous Region of China after having evaluated the forensic application value of these loci in forensic sciences, and then compared the population distinctions between the Kazak group and other reference groups. We observed a total of 240 alleles at these X-STR loci with the corresponding allelic frequencies ranging from 0.0017 to 0.5917. In the study, the highest polymorphism was found at DXS10135 locus. The combined power of discrimination in females was 0.999999999999999999999985 and in males 0.999999999999968. The present study indicates that the 19 X-STR loci are very useful for both forensic identification cases and kinship analyses involving a female offspring. (C) 2016 Elsevier B.V. All rights reserved.

Keyword :

Genetic polymorphisms Haplotypic structure X-STRs Kazak ethnic group

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GB/T 7714 Liu, Yao-Shun , Meng, Hao-Tian , Mei, Ting et al. Genetic diversity and haplotypic structure of Chinese Kazak ethnic group revealed by 19 STRs on the X chromosome [J]. | GENE , 2017 , 600 : 64-69 .
MLA Liu, Yao-Shun et al. "Genetic diversity and haplotypic structure of Chinese Kazak ethnic group revealed by 19 STRs on the X chromosome" . | GENE 600 (2017) : 64-69 .
APA Liu, Yao-Shun , Meng, Hao-Tian , Mei, Ting , Zhang, Li-Ping , Chen, Jian-Gang , Zhang, Yu-Dang et al. Genetic diversity and haplotypic structure of Chinese Kazak ethnic group revealed by 19 STRs on the X chromosome . | GENE , 2017 , 600 , 64-69 .
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