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Oxyphyllanene B overcomes temozolomide resistance in glioblastoma: Structure-activity relationship and mitochondria-associated ER membrane dysfunction. PubMed
期刊论文 | 2022 , 94 , 153816 | Phytomedicine : international journal of phytotherapy and phytopharmacology
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The identification of novel therapeutic candidates from natural products for the development of chemoresistant glioblastoma multiforme (GBM) treatment has been a highly significant and effective strategy.Sesquiterpenes are a class of naturally occurring 15-carbon isoprenoid compounds, and several types of sesquiterpenes have the ability to induce growth inhibition and apoptosis in a variety of cancer cell lines. In the present study, 56 sesquiterpenes of five types, namely, eudesmane-type (I) (1-24), eremophilane-type (II) (25-32), cadinane-type (III) (33-41), guaiane-type (IV) (42-49), and oplopanone-type (V) (50-56), were screened for their antiglioma activity, structure-activity relationship analysis (SAR), and underlying mechanism based on patient-derived recurrent GBM strains, patient-derived GBM cell sphere, GBM organoid (GBO) models, and temozolomide (TMZ)-resistant GBM cell lines.We found that compound 12 (oxyphyllanene B, OLB) showed the most potent antiglioma activity, and we confirmed that OLB could induce apoptosis in a time- and dose-dependent manner in TMZ-resistant GBM cells and GBOs. SAR announced that the presence of an α, β-unsaturated carbonyl moiety was likely to enhance cytotoxic activities. Mechanistic studies demonstrated that OLB induced abnormal changes in ER and mitochondria-associated membrane (MAM) networks, which triggered ER stress, mitochondrial dysfunction, and apoptosis. Furthermore, our findings suggested that OLB-triggered PACS2 activation might form a committed step to disrupt ER-mitochondria communication and showed for the first time that the expression levels of PACS2 might positively correlate with the progression and chemotherapy resistance of glioma.Our results indicated that OLB might be a promising candidate for treating TMZ-resistant GBM cells by activating PACS2, which triggered a crucial event to promote the disruption of ER-mitochondria communication and overcome chemotherapy resistance of GBM.

Keyword :

PACS2 Temozolomide resistance Oxyphyllanene B Glioma ER-mitochondria contacts

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GB/T 7714 Cui Ping , Chen Fanfan , Ma Guoxu et al. Oxyphyllanene B overcomes temozolomide resistance in glioblastoma: Structure-activity relationship and mitochondria-associated ER membrane dysfunction. [J]. | Phytomedicine : international journal of phytotherapy and phytopharmacology , 2022 , 94 : 153816 .
MLA Cui Ping et al. "Oxyphyllanene B overcomes temozolomide resistance in glioblastoma: Structure-activity relationship and mitochondria-associated ER membrane dysfunction." . | Phytomedicine : international journal of phytotherapy and phytopharmacology 94 (2022) : 153816 .
APA Cui Ping , Chen Fanfan , Ma Guoxu , Liu Wenlan , Chen Lei , Wang Sicen et al. Oxyphyllanene B overcomes temozolomide resistance in glioblastoma: Structure-activity relationship and mitochondria-associated ER membrane dysfunction. . | Phytomedicine : international journal of phytotherapy and phytopharmacology , 2022 , 94 , 153816 .
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A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker SCIE
期刊论文 | 2021 , 2021 | INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY
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In this study, a new strategy for the simultaneous quantization of five serotonin 5-hydroxytryptamine receptor antagonists-ondansetron, azasetron, ramosetron, granisetron, and tropisetron-either in infusion samples or in injection dosage form was first established based on high-performance liquid chromatography combined with a quantitative analysis of multiple components by a single marker. The quantitative analysis of multicomponents by a single marker method was conducted with ondansetron as an internal reference substance and performed using relative retention time and ultraviolet spectral similarity as the double indicator. The quantitative analysis of the 5-HT3 receptor antagonists was calculated and investigated based on the relative correction factors. Chromatographic separation was achieved using a C-18 column (150 mm x 4.6 mm, 5.0 mu m), and the mobile phase was composed of acetonitrile-0.05 mol.L-1 potassium dihydrogen phosphate (pH 4.0) (25 : 75) at a flow rate of 1.0 mL.min(-1) and detection wavelengths of 307 nm (ondansetron, azasetron, ramosetron), 302 nm (granisetron), and 285 nm (tropisetron). In addition, the accuracy of the quantitative analysis of multicomponents by a single marker method was compared with an external standard method, and no significant difference was observed between the two methods. The established method is rapid, is easy, and does not require many reference substances, and it can been successfully applied as part of the quality control of the five 5-HT3 receptor antagonists in their injection dosage form and infusion sample drugs in hospitals.

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GB/T 7714 Chen, Fuchao , Fang, Baoxia , Li, Peng et al. A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker [J]. | INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY , 2021 , 2021 .
MLA Chen, Fuchao et al. "A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker" . | INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY 2021 (2021) .
APA Chen, Fuchao , Fang, Baoxia , Li, Peng , Wang, Sicen . A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker . | INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY , 2021 , 2021 .
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Facile synthesis and immobilization of functionalized covalent organic framework-1 for electrochromatographic separation EI SCIE PubMed
期刊论文 | 2021 , 1645 | JOURNAL OF CHROMATOGRAPHY A
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Inspired by the outstanding functions of 3-aminopropyltriethoxysilane (APTES), which can be used for functionalization of both covalent organic frameworks (COFs) and substrate surfaces, herein, a proof-of concept demonstration was carried out by one-step synthesis and immobilization of COF-1 in capillary. COF-1 was grown on the inner wall of capillary using APTES, which played a triple role of catalyst, stabilizer, and connecting arm during the process. The immobilization of COF-1 on silicon surface was confirmed by scanning electron microscopy. Moreover, COF-1 modified capillary (COF-1@capillary) column exhibited excellent performance in the electrochromatographic separation of amino acids. High resolution and separation efficiency (225,378 plates/m for 4-methylbiphenyl) were successfully achieved. Separation of methylbenzene, styrene, ethylbenzene, chlorobenzene, 1,2-dichlorobenzene, 1,2,4-trichlorobenzene, 4methylbiphenyl, naphthalene, and 4-vinylbipheny in the electro-driven mode confirmed the successful growth of COF-1 on the inner wall of capillary. The developed facile method for the immobilization of COF-1 may pave the way for further application prospects of boron-based COFs. (c) 2021 Elsevier B.V. All rights reserved.

Keyword :

One step Surface modification 3-Aminopropyltriethoxysilane Covalent organic framework-1 Capillary electrochromatography

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GB/T 7714 Bao, Tao , Wang, Sicen , Zhang, Nan et al. Facile synthesis and immobilization of functionalized covalent organic framework-1 for electrochromatographic separation [J]. | JOURNAL OF CHROMATOGRAPHY A , 2021 , 1645 .
MLA Bao, Tao et al. "Facile synthesis and immobilization of functionalized covalent organic framework-1 for electrochromatographic separation" . | JOURNAL OF CHROMATOGRAPHY A 1645 (2021) .
APA Bao, Tao , Wang, Sicen , Zhang, Nan , Zhang, Juan . Facile synthesis and immobilization of functionalized covalent organic framework-1 for electrochromatographic separation . | JOURNAL OF CHROMATOGRAPHY A , 2021 , 1645 .
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Hydrophilic carboxyl supported immobilization of UiO-66 for novel bar sorptive extraction of non-steroidal anti-inflammatory drugs in food samples EI SCIE PubMed
期刊论文 | 2021 , 355 | FOOD CHEMISTRY
WoS CC Cited Count: 1
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Herein, the preparation of UiO-66 on frosted glass rod (FGR) was proposed through the coordination interaction of Zr-OH groups and carboxyl sites on FGR. The relative standard deviations (RSDs) of intra-batch and interbatch were below 8.0% (n = 7). UiO-66-modified FGR (UiO-66@FGR) was applied to the extraction and monitoring of five non-steroidal anti-inflammatory drugs (NSAIDs) by coupling to novel bar sorptive extraction (BSE) with ultra-high performance liquid chromatography (UPLC). Sample volume, stirring rate, extraction time, sample pH value, desorption solvent, and desorption time were investigated. NSAIDs (ketoprofen, flurbiprofen, ibuprofen, naproxen, and diclofenac sodium) were determined at a low limit of detection (0.92 ng/mL) over a wide linear range (10?1500 ng/mL). The developed method was used to analyze NSAIDs in sheep muscle, chicken wing, and milk with recoveries of 80.8%-117.2%, RSDs < 6.5%. Fabricated UiO-66@FGR exhibited excellent reproducibility, stability, and good adsorption property towards NSAIDs in food samples.

Keyword :

Non-steroidal anti-inflammatory drugs Bar sorptive extraction UiO-66 Ultra-high performance liquid chromatography

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GB/T 7714 Zhang, Nan , Gao, Yan , Xu, Xianliang et al. Hydrophilic carboxyl supported immobilization of UiO-66 for novel bar sorptive extraction of non-steroidal anti-inflammatory drugs in food samples [J]. | FOOD CHEMISTRY , 2021 , 355 .
MLA Zhang, Nan et al. "Hydrophilic carboxyl supported immobilization of UiO-66 for novel bar sorptive extraction of non-steroidal anti-inflammatory drugs in food samples" . | FOOD CHEMISTRY 355 (2021) .
APA Zhang, Nan , Gao, Yan , Xu, Xianliang , Bao, Tao , Wang, Sicen . Hydrophilic carboxyl supported immobilization of UiO-66 for novel bar sorptive extraction of non-steroidal anti-inflammatory drugs in food samples . | FOOD CHEMISTRY , 2021 , 355 .
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Fingerprint analysis of phenolic acid extract of Salvia miltiorrhiza by digital reference standard analyzer with one or two reference standards SCIE PubMed
期刊论文 | 2021 , 16 (1) | CHINESE MEDICINE
WoS CC Cited Count: 1
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Background Fingerprint analysis and simultaneous multi-components determination are crucial for the holistic quality control of traditional Chinese medicines (TCMs). Yet, reference standards (RS) are often commercially unavailable and with other shortages, which severely impede the application of these technologies. Methods A digital reference standard (DRS) strategy and the corresponding software called DRS analyzer, which supports chromatographic algorithms, spectrum algorithms, and the combination of these algorithms, was developed. The extensive function also enabled the DRS analyzer to recommend the chromatographic column based on big data. Results Various quality control methods of fingerprints of 11 compounds in polyphenolic acid extract of Salvia miltiorrhiza (S. miltiorrhiza) were developed based on DRS analyzer, involving relative retention time (RRT) method, linear calibration using two reference substances (LCTRS) technique, RRT combined with Photon Diode Array (PDA) method, LCTRS combined with PDA method. Additionally, the column database of samples was established. Finally, our data demonstrated that the DRS analyzer could accurately identify 11 compounds of the samples, using only one or two physical RSs. Conclusions The DRS strategy is an automated, intelligent, objective, accurate, eco-friendly, universal, sharing, and promising method for overall quality control of TCMs that requires the usage of fewer RSs.

Keyword :

Relative retention time (RRT) Substitute reference standards Fingerprints digital reference standard (DRS) Linear calibration using two reference substances (LCTRS)

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GB/T 7714 Wang, Qingjun , Yu, Xinlan , Sun, Lei et al. Fingerprint analysis of phenolic acid extract of Salvia miltiorrhiza by digital reference standard analyzer with one or two reference standards [J]. | CHINESE MEDICINE , 2021 , 16 (1) .
MLA Wang, Qingjun et al. "Fingerprint analysis of phenolic acid extract of Salvia miltiorrhiza by digital reference standard analyzer with one or two reference standards" . | CHINESE MEDICINE 16 . 1 (2021) .
APA Wang, Qingjun , Yu, Xinlan , Sun, Lei , Tian, Runtao , He, Huaizhen , Wang, Sicen et al. Fingerprint analysis of phenolic acid extract of Salvia miltiorrhiza by digital reference standard analyzer with one or two reference standards . | CHINESE MEDICINE , 2021 , 16 (1) .
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Inside-Out-Oriented Cell Membrane Biomimetic Magnetic Nanoparticles for High-Performance Drug Lead Discovery EI SCIE PubMed
期刊论文 | 2021 , 93 (22) , 7898-7907 | ANALYTICAL CHEMISTRY
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Biomimetic cell membrane-coated nanoparticles have been broadly applied because of their superior biochemical properties. The right-side-out cell membrane coating manner provides nanoparticles with an immune-evasive stealth function in vivo. However, this acts as a drag for drug discovery when the drug targets are the intracellular domain of transmembrane receptors. Herein, inside-out-oriented cell membrane-coated nanoparticles were prepared for screening tyrosine kinase inhibitors, which specifically interacted with the intracellular kinase domain of the epidermal growth factor receptor. Biotinylated human lung adenocarcinoma epithelial cell membranes specifically interacted with streptavidin-immobilized Fe3O4 magnetic nanoparticles and then formed inside-out-oriented cell membrane-coated magnetic nanoparticles (IOCMMNPs). The cell membrane orientation of the IOCMMNPs was successfully confirmed by immunogold electron microscopy, fluorescently labeled confocal microscopy, sialic acid quantification assay, and the adsorption capacity assay. Moreover, IOCMMNPs possessed satisfactory binding capacity, selectivity, and high sensitivity (limit of detection = 0.4 x 10(-3) mu g mL(-1)). Ultimately, IOCMMNPs successfully targeted two main compounds from Strychnos nux-vomica whose potential antitumor activities were further validated by pharmacological studies. The application of the inside-out cell membrane coating strategy further enhances the drug screening efficiency and broadens the insight and methodologies for drug lead discovery. This inside-out cell membrane coating concept also provides a method for the future development of engineered cell membrane-coated nanotechnology.

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GB/T 7714 Bu, Yusi , Zhang, Xiaolin , Zhu, Aihong et al. Inside-Out-Oriented Cell Membrane Biomimetic Magnetic Nanoparticles for High-Performance Drug Lead Discovery [J]. | ANALYTICAL CHEMISTRY , 2021 , 93 (22) : 7898-7907 .
MLA Bu, Yusi et al. "Inside-Out-Oriented Cell Membrane Biomimetic Magnetic Nanoparticles for High-Performance Drug Lead Discovery" . | ANALYTICAL CHEMISTRY 93 . 22 (2021) : 7898-7907 .
APA Bu, Yusi , Zhang, Xiaolin , Zhu, Aihong , Li, Linhao , Xie, Xiaoyu , Wang, Sicen . Inside-Out-Oriented Cell Membrane Biomimetic Magnetic Nanoparticles for High-Performance Drug Lead Discovery . | ANALYTICAL CHEMISTRY , 2021 , 93 (22) , 7898-7907 .
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Fabrication of acid-resistant imprinted layer on magnetic nanomaterials for selective extraction of chlorogenic acid in Honeysuckle EI SCIE PubMed
期刊论文 | 2021 , 1161 | ANALYTICA CHIMICA ACTA
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The common elution process of molecularly imprinted polymers (MIPs) is carried out in an acidic medium, which greatly affects the stability and reusability of synthetic MIPs, especially for magnetic MIPs. In this study, we fabricated an acid-resistant imprinted layer formed by phase-transitioned lysozyme on magnetic nanomaterials for selective extraction of chlorogenic acid in Honeysuckle, which often coexists with structural analogs. The newly designed acid-resistant imprinted layer can not only protect the internal magnetic core from denudation and dissolution, but also maintain the integrity of the imprinted layer during the elution process. The resultant magnetic MIPs exhibited good stability with no change on morphology after the repeatedly eluting process, and satisfactory reusability that can be used at least ten adsorption-desorption cycles with almost no decrease for adsorption capacity. In addition, the resultant materials possess satisfactory magnetism, uniform morphology with typical core-shell structure, stable crystallization, and good adsorption performance showing on high adsorption amount (10.82 mg g(-1)), fast kinetic equilibrium time (as short as 30 min), and satisfactory selectivity (IF = 2.85, SC > 1.5). At last, the obtained magnetic MIPs as adsorbents coupled with HPLC were successfully used to selective extract CGA in Honeysuckle samples with the high recoveries in the range of 92.0-104.4%, and the contents of CGA in Honeysuckle samples from the different origin are calculated in the range of 0.98%-1.24%. (C) 2021 Elsevier B.V. All rights reserved.

Keyword :

Phase-transitioned lysozyme Surface imprinting Fe3O4-Cu NPs Acid resistance Chlorogenic acid

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GB/T 7714 Gao, Yuan , Tang, Yuhai , Gao, Luyao et al. Fabrication of acid-resistant imprinted layer on magnetic nanomaterials for selective extraction of chlorogenic acid in Honeysuckle [J]. | ANALYTICA CHIMICA ACTA , 2021 , 1161 .
MLA Gao, Yuan et al. "Fabrication of acid-resistant imprinted layer on magnetic nanomaterials for selective extraction of chlorogenic acid in Honeysuckle" . | ANALYTICA CHIMICA ACTA 1161 (2021) .
APA Gao, Yuan , Tang, Yuhai , Gao, Luyao , Niu, Yingying , Gao, Ruixia , Chen, Xiaoyi et al. Fabrication of acid-resistant imprinted layer on magnetic nanomaterials for selective extraction of chlorogenic acid in Honeysuckle . | ANALYTICA CHIMICA ACTA , 2021 , 1161 .
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Cell-based 3D bionic screening by mimicking the drug-receptor interaction environment: In vivo EI SCIE PubMed
期刊论文 | 2021 , 9 (3) , 683-693 | Journal of Materials Chemistry B
WoS CC Cited Count: 1
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Most small-molecule drugs influence cell behavior through their interaction with one or more cellular proteins. The efficacy is unanticipated in the later stages of drug development if small-molecule drugs are discovered in the absence of a biological context. Bionic screening is an in vivo drug-receptor interaction platform that can identify small molecules with recognized activity, improving the likelihood of drug efficacy in the clinic. Here, we report the design of an innovative cell-based bionic screening system using 3D microcarrier cultures to simulate in vivo conditions and facilitate small-molecule drug discovery. Through its combination with HPLC/MS, the method can comprehensively identify small-molecule lead compounds in arbitrarily complex systems in an unbiased manner. In particular, cell-covered microcarriers provide a high-density of cells for affinity performance assessments in the absence of appreciable cell damage and maintain immunogenicity, the 3D structure of which is similar to tissue morphology in vivo, thereby mimicking in vivo drug-receptor interactions. The method is scalable, easy to handle, and requires minimal optimization across a range of different cell lines to realize high-throughput drug screening for the corresponding diseases. This provides a valuable tool for lead compound discovery in more physiologically relevant systems and may address the lack of clinically available drugs. © The Royal Society of Chemistry.

Keyword :

Damage detection Cell culture Molecules Bionics Diagnosis Cells Lead compounds Drug interactions

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GB/T 7714 Wei, Fen , Zhang, Xiaxi , Cui, Ping et al. Cell-based 3D bionic screening by mimicking the drug-receptor interaction environment: In vivo [J]. | Journal of Materials Chemistry B , 2021 , 9 (3) : 683-693 .
MLA Wei, Fen et al. "Cell-based 3D bionic screening by mimicking the drug-receptor interaction environment: In vivo" . | Journal of Materials Chemistry B 9 . 3 (2021) : 683-693 .
APA Wei, Fen , Zhang, Xiaxi , Cui, Ping , Gou, Xilan , Wang, Sicen . Cell-based 3D bionic screening by mimicking the drug-receptor interaction environment: In vivo . | Journal of Materials Chemistry B , 2021 , 9 (3) , 683-693 .
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A Fast and Validated HPLC Method for Simultaneous Determination of Dopamine, Dobutamine, Phentolamine, Furosemide, and Aminophylline in Infusion Samples and Injection Formulations SCIE PubMed
期刊论文 | 2021 , 2021 | JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
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A simple, fast, and validated HPLC method was developed for the simultaneous quantization of five cardiovascular agents: dopamine (DPM), dobutamine (DBM), phentolamine (PTM), furosemide (FSM), and aminophylline (APL) either in infusion samples or in an injection dosage form. The proposed method was achieved with a 150 mm x 4.6 mm, 5.0 mu m C-18 column, by using a simple linear gradient. Mobile phase A was buffer (50 mM KH2PO4) and mobile Phase B was acetonitrile at a flow rate of 1.0 mL/min. The column temperature was kept at 30 degrees C, and the injection volume was 20 mu L. All analytes were separated simultaneously at a retention time (tr) of 3.93, 5.84, 7.06, 8.76, and 9.67 min for DPM, DBM, PTM, FSM, and APL, respectively, with a total run time of less than 15.0 min. The proposed method was validated according to ICH guidelines with respect to accuracy, precision, linearity, limit of detection, limit of quantitation, and robustness. Linearity was obtained over a concentration range of 12.0-240.0, 12.0-240.0, 20.0-200.0, 6.0-240.0, and 10.0-200.0 mu g/mL DPM, DBM, PTM, FSM, and APL, respectively. Interday and intraday accuracy and precision data were recorded in the acceptable limits. The new method has successfully been applied for quantification of all five drugs in their injection dosage form, infusion samples, and for evaluation of the stability of investigated drugs in mixtures for endovenous use. The results of the stability study showed that mixtures of DPM, DBM, PTM, FSM, and APL in 5% glucose or 0.9% sodium chloride injection were stable for 48 hours when stored in polypropylene syringes at 25 degrees C.

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GB/T 7714 Chen, Fuchao , Fang, Baoxia , Wang, Sicen . A Fast and Validated HPLC Method for Simultaneous Determination of Dopamine, Dobutamine, Phentolamine, Furosemide, and Aminophylline in Infusion Samples and Injection Formulations [J]. | JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY , 2021 , 2021 .
MLA Chen, Fuchao et al. "A Fast and Validated HPLC Method for Simultaneous Determination of Dopamine, Dobutamine, Phentolamine, Furosemide, and Aminophylline in Infusion Samples and Injection Formulations" . | JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY 2021 (2021) .
APA Chen, Fuchao , Fang, Baoxia , Wang, Sicen . A Fast and Validated HPLC Method for Simultaneous Determination of Dopamine, Dobutamine, Phentolamine, Furosemide, and Aminophylline in Infusion Samples and Injection Formulations . | JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY , 2021 , 2021 .
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芍药苷对结肠炎小鼠肠道菌群及胆汁酸代谢的调节作用 CSCD
期刊论文 | 2021 , 56 (7) , 1811-1819 | 药学学报
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本研究旨在探讨芍药苷对结肠炎小鼠肠道菌群失调及胆汁酸代谢紊乱的调节作用及相关机制.经西安交通大学伦理委员会审议同意并批准后(批准号:XJTU2019-679),采用3%右旋葡聚糖硫酸钠(DSS)诱导急性结肠炎小鼠模型,同时给药组连续灌胃芍药苷(25、50mg·kg-1·d-1)或5-氨基水杨酸(50mg·kg-1·d-1)7天.实验期间每日监测小鼠体重、粪便黏稠度及便血情况.采用HE染色观察结肠组织病理改变,ELISA检测结肠炎性因子,荧光素异硫氰酸酯(FITC)-葡聚糖检测肠通透性,16S rDNA高通量测序分析肠道菌群组成,最后采用靶向胆汁酸代谢组学方法检测粪便中胆汁酸分子含量的变化.结果表明:芍药苷显著抑制了结肠炎小鼠结肠组织中致炎因子TNF-a、IL-1β、IL-6的水平,促进了抑炎因子IL-10的释放;并且芍药苷可降低结肠炎小鼠的肠通透性,改善肠屏障功能损伤.肠道菌群分析结果显示芍药苷显著改善了 DSS引起的小鼠肠道菌群紊乱,尤其提高乳酸杆菌的相对丰度,降低拟杆菌的相对丰度.此外,芍药苷治疗后显著逆转结肠炎小鼠粪便中胆汁酸分子的代谢紊乱(主要包括胆酸、脱氧胆酸、石胆酸以及β-鼠胆酸).最后,对肠道菌属与粪便中胆汁酸分子水平进行相关性分析发现,乳酸杆菌与具有维持肠道稳态作用的脱氧胆酸和石胆酸呈显著正相关.因此,芍药苷可能通过重塑结肠炎小鼠肠道菌群结构,调节菌群介导的胆汁酸代谢紊乱,进而修复肠屏障损伤、降低肠道炎症,最后对结肠炎小鼠发挥保护作用.

Keyword :

肠道菌群 肠屏障 胆汁酸 结肠炎 芍药苷

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GB/T 7714 王欣 , 朱敏 , 董思晶 et al. 芍药苷对结肠炎小鼠肠道菌群及胆汁酸代谢的调节作用 [J]. | 药学学报 , 2021 , 56 (7) : 1811-1819 .
MLA 王欣 et al. "芍药苷对结肠炎小鼠肠道菌群及胆汁酸代谢的调节作用" . | 药学学报 56 . 7 (2021) : 1811-1819 .
APA 王欣 , 朱敏 , 董思晶 , 许银月 , 景王慧 , 王嗣岑 . 芍药苷对结肠炎小鼠肠道菌群及胆汁酸代谢的调节作用 . | 药学学报 , 2021 , 56 (7) , 1811-1819 .
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