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学者姓名:张彦民

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< Page ,Total 17 >
WWOX activation by toosendanin suppresses hepatocellular carcinoma metastasis through JAK2/Stat3 and Wnt/beta-catenin signaling SCIE PubMed
期刊论文 | 2021 , 513 , 50-62 | CANCER LETTERS
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Abstract :

Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related deaths worldwide. Loss of WW-domain containing oxidoreductase (WWOX) has been proven to be associated with malignant metastasis in patients with HCC. In this study, by using a non-biased CRISPR knockout genetic screen targeting 19,050 human genes, we found that toosendanin (TSN) is a novel druggable WWOX candidate agonist for metastatic HCC patients. We also found that TSN exhibited significant anti-proliferative and anti-metastatic effects on HCC cells in a WWOX-dependent manner. Overexpression and knockdown of WWOX in vitro and in vivo confirmed that the suppression of HCC by TSN involved WWOX. TSN regulated Stat3, DVL2, and GSK3 beta by transforming their interactions with WWOX as demonstrated by a Co-IP assay. TSN accelerated the degradation of beta-catenin by promoting the function of APC, AXIN1, CK1, and GSK3 beta complex. Nuclear translocation of p-Stat3 Y705 and beta-catenin was impeded by the TSN-induced blockade of JAK2/Stat3 and Wnt/beta-catenin signaling, accompanied by the inhibition of MMPs and C-MYC.

Keyword :

Natural products WWOX agonist CRISPR library Screening Pulmonary metastasis HCC

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GB/T 7714 Yang, Tianfeng , Xu, Rui , Huo, Jian et al. WWOX activation by toosendanin suppresses hepatocellular carcinoma metastasis through JAK2/Stat3 and Wnt/beta-catenin signaling [J]. | CANCER LETTERS , 2021 , 513 : 50-62 .
MLA Yang, Tianfeng et al. "WWOX activation by toosendanin suppresses hepatocellular carcinoma metastasis through JAK2/Stat3 and Wnt/beta-catenin signaling" . | CANCER LETTERS 513 (2021) : 50-62 .
APA Yang, Tianfeng , Xu, Rui , Huo, Jian , Wang, Bo , Du, Xia , Dai, Bingling et al. WWOX activation by toosendanin suppresses hepatocellular carcinoma metastasis through JAK2/Stat3 and Wnt/beta-catenin signaling . | CANCER LETTERS , 2021 , 513 , 50-62 .
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Gender- and age-related differences in distinct phenotypes of hypertrophic cardiomyopathy-associated mutation MYBPC3-E334K SCIE PubMed
期刊论文 | 2021 , 36 (10) , 1525-1535 | HEART AND VESSELS
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The mutation MYBPC3-E334K is a culprit mutation of hypertrophic cardiomyopathy (HCM). The pathogenicity of MYBPC3-E334K is conflicting in ClinVar because of the limited segregation data and the relatively high frequency in gnomAD (0.03% overall, with 0.3% in East Asians and 0.8% in Japanese). The main aim is to clarify the clinical importance and phenotype-genotype correlations in subjects with or without MYBPC3-E334K alone. The prevalence of MYBPC3-E334K was sequenced in 1017 HCM unrelated probands. The clinical features, morphology phenotypes, and electrical phenotypes were further analyzed according to the phenotype and genotype status in families with single-mutation MYBPC3-E334K. Nine of 1017 (0.88%) unrelated HCM probands were detected harboring MYBPC3-E334K, and three of them harbored a second variant in sarcomere protein gene. Family study and co-segregation analyses indicated that patients with single-mutation MYBPC3-E334K showed autosomal dominant mode of inheritance with incomplete penetrance. The overall disease penetrance was 52.6%, and the disease penetrance was higher in males than in females (100% in men vs 25% in women, p = 0.003). The mean age at diagnosis of males was approximately 25 years younger than females (36.57 +/- 18.65 vs 62.33 +/- 12.10, p = 0.062). The variant MYBPC3-E334K was classified as a likely pathogenic variant, and a second sarcomere variant did not reveal obvious cumulative effects. The patients harboring single-mutation MYBPC3-E334K had incomplete penetrance, and males demonstrated higher penetrance and early onset HCM than females. A second sarcomere variant did not reveal obvious cumulative effects.

Keyword :

E334K Gender differences MYBPC3&#8211 genotype correlation Incomplete penetrance Phenotype&#8211

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GB/T 7714 Yang, Qian-Li , Zuo, Lei , Ma, Zhi-Ling et al. Gender- and age-related differences in distinct phenotypes of hypertrophic cardiomyopathy-associated mutation MYBPC3-E334K [J]. | HEART AND VESSELS , 2021 , 36 (10) : 1525-1535 .
MLA Yang, Qian-Li et al. "Gender- and age-related differences in distinct phenotypes of hypertrophic cardiomyopathy-associated mutation MYBPC3-E334K" . | HEART AND VESSELS 36 . 10 (2021) : 1525-1535 .
APA Yang, Qian-Li , Zuo, Lei , Ma, Zhi-Ling , Lei, Chang-Hui , Zhu, Xiao-Li , Wang, Xuan-Ying et al. Gender- and age-related differences in distinct phenotypes of hypertrophic cardiomyopathy-associated mutation MYBPC3-E334K . | HEART AND VESSELS , 2021 , 36 (10) , 1525-1535 .
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Sanguinarine impedes metastasis and causes inversion of epithelial to mesenchymal transition in breast cancer SCIE PubMed
期刊论文 | 2021 , 84 | PHYTOMEDICINE
WoS CC Cited Count: 1
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Abstract :

Background: A large number of breast cancer patients perishes due to metastasis instead of primary tumor, but molecular mechanisms contributing towards cancer metastasis remain poorly understood. Therefore, prompting development of novel treatment is inevitable. A vast variety of plant derived natural substance possesses several therapeutically active constituents, e.g. alkaloids, flavonoids, tannins, resins, terpenoids etc. that exhibit various pharmacological properties e.g. anti-inflammatory, anti-microbial and anti-cancer properties. Sanguinarine (SAN) alkaloid found its place among such naturally occurring substances that exerts several pharmacological activities, including anti-cancer effects. Purpose: Until now, role of SAN not only against epithelial-mesenchymal transition (EMT) but also against metastasis progression in breast cancer remains indistinct. Thus, aim of the present study was to investigate effects of SAN on EMT process and cancer metastasis in animal model. Methods: MTT assay was performed to assess SAN effects on proliferation in breast cancer. Scratch assay was performed to evaluate effects of SAN on migration in breast cancer. Colony formation assay was performed to determine effects of SAN on colonization characteristics of breast cancer. Western blotting was performed to measure EMT regulating protein expression as well as major pathway protein expression induced against TGF-beta treatment in breast cancer. Tail vein method of injecting breast cancer cells in bulb/c mice was conducted to study metastasis progression and thereafter assessing effects of SAN against metastasis in mice. Results: In vivo results: MTT assay performed, demonstrated dose dependent inhibition of cell proliferation in breast cancer. Scratch assay results showed, SAN played a major role as migration inhibitor in estrogen receptor positive (ER+) breast cancer. Colony forming assay results demonstrated that SAN constrains ability of breast cancer to develop into well-defined colonies. Western blotting results for EMT regulating protein expression, after TGF-beta treatment showed, SAN inhibited cadherin switch in ER+ breast cancer. Moreover, expression of pathway proteins involved in EMT process after TGF-beta treatment i.e. Smad, PI3K/Akt and MAP kinase were significantly masked against SAN treatment. In vivo results: The appearance of metastatic nodules in lung tissues of mice model, helps to study the effects of SAN against metastasis in bulb/c mice. The obtained results have confirmed that SAN impeded lung metastasis. The macroscopic examination has confirmed metastasis inhibitory role of SAN in breast cancer. The Hematoxylin and eosin (H&E) staining results further advocate anti-metastatic characteristics of SAN, presented by fewer metastatic nodule and lesions appearance in SAN treated mice compared to untreated metastasis mice. Conclusion: In summary, SAN displayed prominent anti-metastatic effects in animal model and anti-proliferation effects together with significant inhibitory potential on EMT regulating protein expression against TGF-beta treatment in ER+ breast cancer. So, overall findings of our study highlighted the pre-clinical significance of SAN in animal model therefore, further studies in humans as a part of clinical trial will be needed to establish pharmacokinetics and other effects of SAN, so that it can be a potential candidate for future treatment of metastatic breast cancer (MBC).

Keyword :

Breast cancer Sanguinarine Migration EMT Metastasis

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GB/T 7714 Ghauri, Mohsin Ahmad , Su, Qi , Ullah, Asmat et al. Sanguinarine impedes metastasis and causes inversion of epithelial to mesenchymal transition in breast cancer [J]. | PHYTOMEDICINE , 2021 , 84 .
MLA Ghauri, Mohsin Ahmad et al. "Sanguinarine impedes metastasis and causes inversion of epithelial to mesenchymal transition in breast cancer" . | PHYTOMEDICINE 84 (2021) .
APA Ghauri, Mohsin Ahmad , Su, Qi , Ullah, Asmat , Wang, Jingjing , Sarwar, Ammar , Wu, Qing et al. Sanguinarine impedes metastasis and causes inversion of epithelial to mesenchymal transition in breast cancer . | PHYTOMEDICINE , 2021 , 84 .
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Cephalomannine inhibits hypoxia-induced cellular function via the suppression of APEX1/HIF-1 alpha interaction in lung cancer SCIE PubMed
期刊论文 | 2021 , 12 (5) | CELL DEATH & DISEASE
WoS CC Cited Count: 1
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Lung cancer (LC) is one of the leading causes of cancer-related death. As one of the key features of tumor microenvironment, hypoxia conditions are associated with poor prognosis in LC patients. Upregulation of hypoxic-induced factor-1 alpha (HIF-1 alpha) leads to the activation of various factors that contribute to the increased drug resistance, proliferation, and migration of tumor cells. Apurinic/apyrimidinic endonuclease-1 (APEX1) is a multi-functional protein that regulates several transcription factors, including HIF-1 alpha, that contribute to tumor growth, oxidative stress responses, and DNA damage. In this study, we explored the mechanisms underlying cell responses to hypoxia and modulation of APEX1, which regulate HIF-1 alpha and downstream pathways. We found that hypoxia-induced APEX1/HIF-1 alpha pathways regulate several key cellular functions, including reactive oxygen species (ROS) production, carbonic anhydrase 9 (CA9)-mediated intracellular pH, migration, and angiogenesis. Cephalomannine (CPM), a natural compound, exerted inhibitory effects in hypoxic LC cells via the inhibition of APEX1/HIF-1 alpha interaction in vitro and in vivo. CPM can significantly inhibit cell viability, ROS production, intracellular pH, and migration in hypoxic LC cells as well as angiogenesis of HUVECs under hypoxia through the inhibition of APEX1/HIF-1 alpha interaction. Taken together, CPM could be considered as a promising compound for LC treatment.

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GB/T 7714 Ullah, Asmat , Leong, Sze Wei , Wang, Jingjing et al. Cephalomannine inhibits hypoxia-induced cellular function via the suppression of APEX1/HIF-1 alpha interaction in lung cancer [J]. | CELL DEATH & DISEASE , 2021 , 12 (5) .
MLA Ullah, Asmat et al. "Cephalomannine inhibits hypoxia-induced cellular function via the suppression of APEX1/HIF-1 alpha interaction in lung cancer" . | CELL DEATH & DISEASE 12 . 5 (2021) .
APA Ullah, Asmat , Leong, Sze Wei , Wang, Jingjing , Wu, Qing , Ghauri, Mohsin Ahmad , Sarwar, Ammar et al. Cephalomannine inhibits hypoxia-induced cellular function via the suppression of APEX1/HIF-1 alpha interaction in lung cancer . | CELL DEATH & DISEASE , 2021 , 12 (5) .
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Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1 alpha pathways in breast cancer SCIE PubMed
期刊论文 | 2021 , 84 | PHYTOMEDICINE
WoS CC Cited Count: 3
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Background: Breast cancer is the most common female cancer worldwide. Large hypoxic area is one of the features of tumor microenvironment. Highly activated hypoxia-induced pathways positively correlate with poor clinical response to chemo- and radiotherapy and high mortality in breast cancer patients. Purpose: We explore the effect of sanguinarine on hypoxia-induced activation of Ephrin type-B receptor 4 (EphB4) and hypoxia inducible factor-1 alpha (HIF-1 alpha) pathways in breast cancer. Results: Hypoxia-induced expression of a receptor tyrosine kinase EphB4 was observed in hypoxic breast cancer cell models. Sanguinarine, a natural alkaloid, could effectively combat hypoxia-induced EphB4 and HIF-1 alpha expression. Sanguinarine inhibited the activation of downstream protein signal transducer and activator of transcription-3 (STAT3), thereby blocking hypoxia-induced HIF-1 alpha/STAT3 interaction and downregulating the mRNA levels of their target genes. Mechanically, sanguinarine attenuated HIF-1 alpha protein levels via inhibition of MAPK/ERK pathways and promotion of HIF-1 alpha proteasome degradation. Sanguinarine inhibited STAT3 activation through targeting its upstream EphB4 and accelerating STAT3 dephosphorylation. Correspondingly, xenograft models confirmed that sanguinarine treatment disrupted hypoxia-induced pathways and inhibited tumor growth in vivo. Conclusions: Our results may bring insights to the hypoxia-induced pathways in breast cancers, and suggest sanguinarine as a promising candidate for EphB4 and HIF-1 alpha-targeted inhibition.

Keyword :

Breast cancer Sanguinarine Ephrin type-B receptor 4 Hypoxia inducible factor-1 alpha Signal transducer and activator of transcription-3

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GB/T 7714 Su, Qi , Wang, Jingjing , Wu, Qing et al. Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1 alpha pathways in breast cancer [J]. | PHYTOMEDICINE , 2021 , 84 .
MLA Su, Qi et al. "Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1 alpha pathways in breast cancer" . | PHYTOMEDICINE 84 (2021) .
APA Su, Qi , Wang, Jingjing , Wu, Qing , Ullah, Asmat , Ghauri, Mohsin Ahmad , Sarwar, Ammar et al. Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1 alpha pathways in breast cancer . | PHYTOMEDICINE , 2021 , 84 .
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beta(2)-adrenergic receptor affinity chromatography with an interaction force analysis model: A method for analysis of active compounds targeting beta(2)-adrenergic receptor EI SCIE PubMed
期刊论文 | 2021 , 1652 | JOURNAL OF CHROMATOGRAPHY A
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Asthma is one of the most prevalent diseases worldwide, and beta(2)-adrenergic receptor (beta(2)AR) agonists have been reported to be highly effective bronchodilators against this disease. In this study, we successfully constructed a novel CHO-beta(2)AR affinity chromatography (CHO-beta(2)AR/AC), which was evaluated by infrared spectroscopic and scanning electron microscope (SEM) analysis. In addition, CHO-beta(2)AR/AC model exhibited good selectivity and reliability with the relative standard deviation smaller than 5.6% after 30 days. Furthermore, an interaction force analysis model was developed based on CHO-beta(2)AR/AC. The results showed that the interaction force analysis model (Phi center dot E center dot pKa) exhibited a strong correlation with equilibrium dissociation constant (K-D) (r(2)=0.9284, p=0.002) and a good correlation with logarithm of half-maximum effective concentration (pEC(50)) values (r(2)=0.7135, p=0.034). In addition, a pool of clinically approved drugs was screened by this CHO-beta(2)AR/AC model. Codeine was found to bind to and activate beta(2)AR with K-D value of 4.10 x 10(-7) M, leading to increased cyclic adenosine monophosphate (cAMP) production with EC50 of 6.49 x 10(-7) M and reduction of intracellular Ca-2 + concentration, which in turn relaxes bronchial contraction with EC50 of 2.62 x 10(- 6) M. Furthermore, the K-D value and pEC(50) of codeine were within the 95% prediction range of the interaction force analysis model. The results indicate that the CHO-beta(2)AR/AC with interaction force analysis model constructed in this study can be used to effectively and rapidly screen active compounds targeting beta(2)AR. (C) 2021 Elsevier B.V. All rights reserved.

Keyword :

Drug-receptor interaction Affinity chromatography beta(2) AR Interaction force analysis model Codeine

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GB/T 7714 Ma, Weina , Yang, Liu , Liu, Yanhong et al. beta(2)-adrenergic receptor affinity chromatography with an interaction force analysis model: A method for analysis of active compounds targeting beta(2)-adrenergic receptor [J]. | JOURNAL OF CHROMATOGRAPHY A , 2021 , 1652 .
MLA Ma, Weina et al. "beta(2)-adrenergic receptor affinity chromatography with an interaction force analysis model: A method for analysis of active compounds targeting beta(2)-adrenergic receptor" . | JOURNAL OF CHROMATOGRAPHY A 1652 (2021) .
APA Ma, Weina , Yang, Liu , Liu, Yanhong , Lei, Panpan , Zhang, Yanmin . beta(2)-adrenergic receptor affinity chromatography with an interaction force analysis model: A method for analysis of active compounds targeting beta(2)-adrenergic receptor . | JOURNAL OF CHROMATOGRAPHY A , 2021 , 1652 .
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The facile formation of hierarchical mesoporous silica nanocarriers for tumor-selective multimodal theranostics EI SCIE PubMed
期刊论文 | 2021 , 9 (15) , 5237-5246 | BIOMATERIALS SCIENCE
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The combination of therapeutic and diagnostic functions in a single platform has aroused great interest due to the more optimal synergistic effects that can be obtained as compared to any single theranostic approach alone. However, current nanotheranostics are normally formed via complicated construction steps involving the pre-synthesis of each component and further conjugation via chemical bonds, which may cause low integration efficiency and limit production and applications. Herein, a tumor-targeting and tumor-responsive all-in-one nanoplatform based on mesoporous silica nanocarriers (MSNs) was fabricated via a facile approach utilizing efficient and nondestructive physical interactions for long-wavelength fluorescence imaging-guided synergistic chemo-catalytic-photothermal tumor therapy. The MSNs were endowed with these multimodal theranostics via a simple hydrothermal method after coordinating with Fe2+ and glutathione (GSH) to introduce ferroferric oxide and carbon dots in situ. The former acts as a photothermal agent and catalytic agent to generate local heat under 808 nm irradiation and also when toxic hydroxyl radicals (OH) are in contact with abundant hydrogen peroxide in cancer cells, while the latter participates in fluorescence imaging. After loading with paclitaxel (PTX), polyester and folic-acid-conjugated cyclodextrin were employed to serve as an esterase-sensitive gatekeeper controlling PTX release from the MSN pores and as a tumor-targeting agent for accurate therapy, respectively. As expected, the nanoplatform was efficiently taken up by tumor cells over healthy cells, and then, synergetic chemo-catalytic-photothermal therapy was performed, resulting in 5-fold greater apoptosis of tumor cells as compared to healthy cells under 808 nm irradiation. Moreover, in vivo data from tumor-bearing mouse models showed that tumors were significantly inhibited, and the survival rates of these mice increased to greater than 80% after 5 weeks of treatment with our nanoplatform. These therapeutic processes could be directly tracked via fluorescence imaging enabled by carbon dots and, therefore, our nanoplatform provides a promising theranostics approach for tumor treatment.

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GB/T 7714 Guo, Xiaoyan , Zhu, Man , Yuan, Pingyun et al. The facile formation of hierarchical mesoporous silica nanocarriers for tumor-selective multimodal theranostics [J]. | BIOMATERIALS SCIENCE , 2021 , 9 (15) : 5237-5246 .
MLA Guo, Xiaoyan et al. "The facile formation of hierarchical mesoporous silica nanocarriers for tumor-selective multimodal theranostics" . | BIOMATERIALS SCIENCE 9 . 15 (2021) : 5237-5246 .
APA Guo, Xiaoyan , Zhu, Man , Yuan, Pingyun , Liu, Tao , Tian, Ran , Bai, Yongkang et al. The facile formation of hierarchical mesoporous silica nanocarriers for tumor-selective multimodal theranostics . | BIOMATERIALS SCIENCE , 2021 , 9 (15) , 5237-5246 .
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Selenium sulfide disrupts the PLAGL2/C-MET/STAT3-induced resistance against mitochondrial apoptosis in hepatocellular carcinoma SCIE PubMed
期刊论文 | 2021 , 11 (9) | CLINICAL AND TRANSLATIONAL MEDICINE
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Background Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths worldwide. Overexpression of pleomorphic adenoma gene like-2 (PLAGL2) is associated with tumorigenesis. However, its function in HCC is unclear, and there are currently no anti-HCC drugs that target PLAGL2. Drug repositioning may facilitate the development of PLAGL2-targeted drug candidates. Methods The expression of PLAGL2 in HCC clinical tissue samples and HCC cell lines was analyzed by western blotting. The constructed HCC cell models were used to confirm the underlying function of PLAGL2 as a therapeutic target. Multiple in vitro and in vivo assays were conducted to determine the anti-proliferative and apoptosis-inducing effects of selenium sulfide (SeS2), which is clinically used for the treatment of seborrheic dermatitis and tinea versicolor. Results PLAGL2 expression was higher in HCC tumor tissues than in normal adjacent tissues. Its overexpression promoted the resistance of HCC cells of mitochondrial apoptosis through the regulation of the downstream C-MET/STAT3 signaling axis. SeS2 exerted significant anti-proliferative and apoptosis-inducing effects on HCC cells in a PLAGL2-dependent manner. Mechanistically, SeS2 suppressed C-MET/STAT3, AKT/mTOR, and MAPK signaling and triggered Bcl-2/Cyto C/Caspase-mediated intrinsic mitochondrial apoptosis both in vitro and in vivo. Conclusions Our data reveal an important role of PLAGL2 in apoptosis resistance in HCC and highlight the potential of using SeS2 as a PLAGL2 inhibitor in patients with HCC.

Keyword :

C-MET PLAGL2 selenium sulfide HCC STAT3

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GB/T 7714 Yang, Tianfeng , Huo, Jian , Xu, Rui et al. Selenium sulfide disrupts the PLAGL2/C-MET/STAT3-induced resistance against mitochondrial apoptosis in hepatocellular carcinoma [J]. | CLINICAL AND TRANSLATIONAL MEDICINE , 2021 , 11 (9) .
MLA Yang, Tianfeng et al. "Selenium sulfide disrupts the PLAGL2/C-MET/STAT3-induced resistance against mitochondrial apoptosis in hepatocellular carcinoma" . | CLINICAL AND TRANSLATIONAL MEDICINE 11 . 9 (2021) .
APA Yang, Tianfeng , Huo, Jian , Xu, Rui , Su, Qi , Tang, Wenjuan , Zhang, Dongdong et al. Selenium sulfide disrupts the PLAGL2/C-MET/STAT3-induced resistance against mitochondrial apoptosis in hepatocellular carcinoma . | CLINICAL AND TRANSLATIONAL MEDICINE , 2021 , 11 (9) .
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Induction of estrogen receptor beta-mediated autophagy sensitizes breast cancer cells to TAD1822-7, a novel biphenyl urea taspine derivative SCIE PubMed
期刊论文 | 2021 | MOLECULAR BIOLOGY REPORTS
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Background Female breast cancer has become the most commonly diagnosed cancer worldwide. As a tumor suppressor, estrogen receptor beta (ER beta) can be potentially targeted for breast cancer therapy. Methods and results TAD1822-7 was evaluated for ER beta-mediated autophagy and cell death using cell proliferation assay, Annexin V/PI staining, immunofluorescence, western blotting, ER beta siRNA, ER beta plasmid transfection and hypoxia cell models. TAD1822-7 upregulated ER beta causing cell death and induced mitochondrial dysfunction and autophagy companied with mitochondrial located ER beta. Enhanced levels of microtubule associated protein1 light chain 3 (LC3)-II and p62/SQSTM1 (p62) indicated that TAD1822-7 blocked the late-stage autolysosome formation, leading to cell death. Mechanistically, TAD1822-7-induced cell death was mediated by phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathways. Moreover, TAD1822-7 modulated hypoxia inducible factor (HIF) functions and autophagy via the inhibition of HIF-1 beta in the context of hypoxia-induced autophagy. ER beta overexpression and ER beta agonist showed similar effects, whereas ER beta siRNA abrogated TAD1822-7-induced cell death, the inhibition of PI3K/AKT pathway and autophagy. The involvement of PI3K/AKT pathway and autophagy was also demonstrated in TAD1822-7-treated hypoxic breast cancer cells. Conclusions These findings provide new insight into the mechanism underlying the inhibitory effects of TAD1822-7 via ER beta-mediated pathways in breast cancer cells.

Keyword :

Autophagy Estrogen receptor beta Hypoxia Breast cancer Cell death TAD1822-7

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GB/T 7714 Su, Qi , Wu, Qing , Chen, Kun et al. Induction of estrogen receptor beta-mediated autophagy sensitizes breast cancer cells to TAD1822-7, a novel biphenyl urea taspine derivative [J]. | MOLECULAR BIOLOGY REPORTS , 2021 .
MLA Su, Qi et al. "Induction of estrogen receptor beta-mediated autophagy sensitizes breast cancer cells to TAD1822-7, a novel biphenyl urea taspine derivative" . | MOLECULAR BIOLOGY REPORTS (2021) .
APA Su, Qi , Wu, Qing , Chen, Kun , Wang, Jingjing , Sarwar, Ammar , Zhang, Yanmin . Induction of estrogen receptor beta-mediated autophagy sensitizes breast cancer cells to TAD1822-7, a novel biphenyl urea taspine derivative . | MOLECULAR BIOLOGY REPORTS , 2021 .
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Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2 SCIE PubMed
期刊论文 | 2021 , 56 | STEM CELL RESEARCH
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Induced pluripotent stem cell lines (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the peripheral blood of a ten years old boy with the type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2. PBMCs were reprogrammed using non-integrative Sendai viral vectors containing reprogramming factors OCT4, SOX2, KLF4 and C-MYC. The iPSCs were shown to express pluripotent markers, have trilineage differentiation potential, carry c.1814C>T(p.P605L) mutation in KCNH2 and have a normal karyotype. Thuse the iPSC line we established will be useful for studying the patho-genesis of the type 2 long QT syndrome and drug testing.

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GB/T 7714 Wang, Tao , Zhou, Yafei , Zhou, Rui et al. Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2 [J]. | STEM CELL RESEARCH , 2021 , 56 .
MLA Wang, Tao et al. "Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2" . | STEM CELL RESEARCH 56 (2021) .
APA Wang, Tao , Zhou, Yafei , Zhou, Rui , Huang, Wenjun , Wang, Jie , Li, Huan et al. Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2 . | STEM CELL RESEARCH , 2021 , 56 .
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