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< Page ,Total 34 >
Developing hierarchical porous organic polymers with tunable nitrogen base sites via theoretical calculation-directed monomers selection for efficient capture and catalytic utilization of CO2 EI SCIE
期刊论文 | 2021 , 420 | CHEMICAL ENGINEERING JOURNAL
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Abstract :

We report herein a calculation-directed strategy to select nitrogen-containing monomers for the construction of nitrogen-doped hierarchical porous organic polymers (H-POPs) through alkylation-induced hypercross-linking of nitrogen-containing monomers. With the assistance of DFT calculations, four nitrogen-containing monomers with relatively strong interactions with CO2 were identified. The as-designed H-POPs are large in BET surface areas (768 similar to 1177 m(2)/g) and have abundant hierarchical nanopores. The nature of nitrogen sites in H-POPs is precise and tunable, offering a great opportunity to investigate the criteria that are critical for the selective capture of CO2. The synthesized H-POPs exhibit extraordinary CO2 capacities (2.61 mmol/g at 0 degrees C and 0.15 bar), satisfactory IAST CO2/N-2 selectivities (79.8 at 25 degrees C) and superb breakthrough performance for the separation of CO2 from simulated flue gas. These characteristics are beneficial for the application of H-POPs in the catalytic cycloaddition of CO2 to cyclic carbonate after metallization.

Keyword :

CO2 capture Nitrogen base site Porous organic polymer DFT calculation CO2 conversion

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GB/T 7714 Kong, Liyun , Han, Shengli , Zhang, Tao et al. Developing hierarchical porous organic polymers with tunable nitrogen base sites via theoretical calculation-directed monomers selection for efficient capture and catalytic utilization of CO2 [J]. | CHEMICAL ENGINEERING JOURNAL , 2021 , 420 .
MLA Kong, Liyun et al. "Developing hierarchical porous organic polymers with tunable nitrogen base sites via theoretical calculation-directed monomers selection for efficient capture and catalytic utilization of CO2" . | CHEMICAL ENGINEERING JOURNAL 420 (2021) .
APA Kong, Liyun , Han, Shengli , Zhang, Tao , He, Langchong , Zhou, Linsen . Developing hierarchical porous organic polymers with tunable nitrogen base sites via theoretical calculation-directed monomers selection for efficient capture and catalytic utilization of CO2 . | CHEMICAL ENGINEERING JOURNAL , 2021 , 420 .
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Dictamnine is an effective anti-anaphylactoid compound acting via the MrgX2 receptor located on mast cells SCIE PubMed
期刊论文 | 2021 , 35 (6) , 3181-3193 | PHYTOTHERAPY RESEARCH
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Abstract :

Anaphylactoid reactions are potentially fatal allergic diseases caused by mast cells (MCs), which release histamine and lipid mediators under certain stimuli. Therefore, there is an urgent need to develop new drug candidates to treat anaphylactoid reactions. The MrgX2 receptor mediates anaphylactoid reactions that cause inflammatory diseases. Cortex dictamni is a Chinese herb used for treating allergy-related diseases; however, its active compound is still unknown and its mechanism of action has not yet been reported. The aim of this study was to screen the anti-anaphylactoid compound from C. dictamni extracts. An MrgX2/CMC-HPLC method was established for screening MrgX2-specific compounds retained from the alcohol extract of C. dictamni. A mouse model of hindpaw extravasation was used to evaluate the anti-anaphylactoid effect of this ingredient. Intracellular Ca2+ mobilization was assessed using a calcium imaging assay. Enzyme immunoassays were performed to measure cytokine and chemokine release levels. The molecular signaling pathways were explored by western blotting. As a result, dictamnine was identified as an effective compound using the MrgX2/CMC method, which remarkably suppressed MC intracellular Ca2+ mobilization and the release of de novo degranulated substances, and inhibited PKC-PLC gamma-IP3R-associated protein signaling molecules. Hence, dictamnine is a novel therapeutic candidate for anaphylactoid reactions via MrgX2.

Keyword :

MrgX2 anaphylactoid effects dictamnine mast cells CMC anti&#8208

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GB/T 7714 Liu, Rui , Hu, Shiling , Ding, Yuanyuan et al. Dictamnine is an effective anti-anaphylactoid compound acting via the MrgX2 receptor located on mast cells [J]. | PHYTOTHERAPY RESEARCH , 2021 , 35 (6) : 3181-3193 .
MLA Liu, Rui et al. "Dictamnine is an effective anti-anaphylactoid compound acting via the MrgX2 receptor located on mast cells" . | PHYTOTHERAPY RESEARCH 35 . 6 (2021) : 3181-3193 .
APA Liu, Rui , Hu, Shiling , Ding, Yuanyuan , Wang, Jue , Wang, Yuejin , Gao, Jiapan et al. Dictamnine is an effective anti-anaphylactoid compound acting via the MrgX2 receptor located on mast cells . | PHYTOTHERAPY RESEARCH , 2021 , 35 (6) , 3181-3193 .
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Advances in cell membrane chromatography EI SCIE PubMed
期刊论文 | 2021 , 1639 | Journal of Chromatography A
WoS CC Cited Count: 1
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Cell membrane chromatography (CMC) is a biomimetic chromatographic method based on the ability of membrane receptors to selectively interact with their ligands in vivo. Using membrane receptors as a stationary phase, the CMC method helps in determining the binding characteristics between ligands and membrane receptors and in efficiently identifying specific target components in a complex sample that produce the cellular biological effects of ligands (drugs, antibodies, enzymes, cytokines, etc.). CMC is an analytical tool for revealing characteristics of ligand–receptor interactions, screening and discovering target substances, and accurately controlling the quality of drugs. Since establishment of CMC in the early 1990s, with the rapid development of cell biology, significant progress has been made in the development of high-expression receptors, engineered cell cultures, and standardized preparations, which allowed in vitro immobilization of cell membrane receptors and miniaturization of binding assays. A variety of CMC models have been established using different membrane receptors as a stationary phase, and many new methods have been developed by combining CMC with high-performance liquid chromatography (HPLC)/mass spectrometry or HPLC-IT-TOF technologies. CMC methods have been widely used to study drug–receptor interactions and to screen complex samples for effective or harmful components. © 2021 Elsevier B.V.

Keyword :

Biomimetic processes Cell immobilization Cells Ligands Chromatographic analysis Drug interactions Membranes High performance liquid chromatography

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GB/T 7714 Ma, Weina , Wang, Cheng , Liu, Rui et al. Advances in cell membrane chromatography [J]. | Journal of Chromatography A , 2021 , 1639 .
MLA Ma, Weina et al. "Advances in cell membrane chromatography" . | Journal of Chromatography A 1639 (2021) .
APA Ma, Weina , Wang, Cheng , Liu, Rui , Wang, Nan , Lv, Yanni , Dai, Bingling et al. Advances in cell membrane chromatography . | Journal of Chromatography A , 2021 , 1639 .
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Research on the Adaptive Sensitivity Scanning Method for Ion Conductance Microscopy with High Efficiency and Reliability SCIE PubMed
期刊论文 | 2021 , 93 (36) , 12296-12304 | ANALYTICAL CHEMISTRY
WoS CC Cited Count: 1
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Abstract :

Scanning ion conductance microscopy (SICM) is a type of in situ measurement technology for noncontact detection of samples in electrolytes with nanoscale resolution and has been used increasingly in biomedical and electrochemical fields in recent years. However, there is an inherent contradiction in the technique that makes SICM's sensitivity and accuracy difficult to balance. Higher sensitivity allows for faster probe speeds and higher scanning reliability but leads to lower accuracy, and vice versa. To resolve this problem, an adaptive sensitivity scanning method is proposed here that is designed to increase SICM's imaging efficiency without reducing its scanning reliability and accuracy. In the proposed scanning method, the sensitivity is automatically switched via the bias voltage based on the probe-sample distance. When the probe is located far away from the sample, the probe then predetects the sample position rapidly with high sensitivity. When the sample has been sensed in the high-sensitivity phase, the probe then detects the sample with low sensitivity. The basic theory and the feasibility of the alterable sensitivity detection strategy is also studied using the finite element method (FEM) and by performing experiments in this work. Finally, through testing of the standard silicon and polydimethylsiloxane (PDMS) samples, the proposed method is shown to increase SICM imaging efficiency significantly by up to 5 times relative to the conventional hopping mode without sacrificing the scanning accuracy and reliability.

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GB/T 7714 Jiao, Yangbohan , Zhuang, Jian , Zhang, Tao et al. Research on the Adaptive Sensitivity Scanning Method for Ion Conductance Microscopy with High Efficiency and Reliability [J]. | ANALYTICAL CHEMISTRY , 2021 , 93 (36) : 12296-12304 .
MLA Jiao, Yangbohan et al. "Research on the Adaptive Sensitivity Scanning Method for Ion Conductance Microscopy with High Efficiency and Reliability" . | ANALYTICAL CHEMISTRY 93 . 36 (2021) : 12296-12304 .
APA Jiao, Yangbohan , Zhuang, Jian , Zhang, Tao , He, Langchong . Research on the Adaptive Sensitivity Scanning Method for Ion Conductance Microscopy with High Efficiency and Reliability . | ANALYTICAL CHEMISTRY , 2021 , 93 (36) , 12296-12304 .
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Three salvianolic acids inhibit 2019-nCoV spike pseudovirus viropexis by binding to both its RBD and receptor ACE2 SCIE PubMed
期刊论文 | 2021 , 93 (5) , 3143-3151 | JOURNAL OF MEDICAL VIROLOGY
WoS CC Cited Count: 3
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Since December 2019, the new coronavirus (also known as severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2, 2019-nCoV])-induced disease, COVID-19, has spread rapidly worldwide. Studies have reported that the traditional Chinese medicine Salvia miltiorrhiza possesses remarkable antiviral properties; however, the anti-coronaviral activity of its main components, salvianolic acid A (SAA), salvianolic acid B (SAB), and salvianolic acid C (SAC) is still debated. In this study, we used Cell Counting Kit-8 staining and flow cytometry to evaluate the toxicity of SAA, SAB, and SAC on ACE2 (angiotensin-converting enzyme 2) high-expressing HEK293T cells (ACE2(h) cells). We found that SAA, SAB, and SAC had a minor effect on the viability of ACE2(h) cells at concentrations below 100 mu M. We further evaluated the binding capacity of SAA, SAB, and SAC to ACE2 and the spike protein of 2019-nCoV using molecular docking and surface plasmon resonance. They could bind to the receptor-binding domain (RBD) of the 2019-nCoV with a binding constant (K-D) of (3.82 +/- 0.43) e-6 M, (5.15 +/- 0.64)e-7 M, and (2.19 +/- 0.14)e-6 M; and bind to ACE2 with K-D (4.08 +/- 0.61)e-7 M, (2.95 +/- 0.78)e-7 M, and (7.32 +/- 0.42)e-7 M, respectively. As a result, SAA, SAB, and SAC were determined to inhibit the entry of 2019-nCoV Spike pseudovirus with an EC50 of 11.31, 6.22, and 10.14 mu M on ACE2(h) cells, respectively. In conclusion, our study revealed that three Salvianolic acids can inhibit the entry of 2019-nCoV spike pseudovirus into ACE2(h) cells by binding to the RBD of the 2019-nCoV spike protein and ACE2 protein.

Keyword :

salvianolic acid B salvianolic acid A nCoV ACE2 salvianolic acid C 2019&#8208

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GB/T 7714 Hu, Shiling , Wang, Jue , Zhang, Yongjing et al. Three salvianolic acids inhibit 2019-nCoV spike pseudovirus viropexis by binding to both its RBD and receptor ACE2 [J]. | JOURNAL OF MEDICAL VIROLOGY , 2021 , 93 (5) : 3143-3151 .
MLA Hu, Shiling et al. "Three salvianolic acids inhibit 2019-nCoV spike pseudovirus viropexis by binding to both its RBD and receptor ACE2" . | JOURNAL OF MEDICAL VIROLOGY 93 . 5 (2021) : 3143-3151 .
APA Hu, Shiling , Wang, Jue , Zhang, Yongjing , Bai, Haoyun , Wang, Cheng , Wang, Nan et al. Three salvianolic acids inhibit 2019-nCoV spike pseudovirus viropexis by binding to both its RBD and receptor ACE2 . | JOURNAL OF MEDICAL VIROLOGY , 2021 , 93 (5) , 3143-3151 .
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RssB-mediated σS Activation is Regulated by a Two-Tier Mechanism via Phosphorylation and Adaptor Protein - IraD. PubMed SCIE
期刊论文 | 2021 , 433 (3) | Journal of molecular biology
WoS CC Cited Count: 1
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Abstract :

Regulation of bacterial stress responding σS is a sophisticated process and mediated by multiple interacting partners. Controlled proteolysis of σS is regulated by RssB which maintains minimal level of σS during exponential growth but then elevates σS level while facing stresses. Bacteria developed different strategies to regulate activity of RssB, including phosphorylation of itself and production of anti-adaptors. However, the function of phosphorylation is controversial and the mechanism of anti-adaptors preventing RssB-σS interaction remains elusive. Here, we demonstrated the impact of phosphorylation on the activity of RssB and built the RssB-σS complex model. Importantly, we showed that the phosphorylation site - D58 is at the interface of RssB-σS complex. Hence, mutation or phosphorylation of D58 would weaken the interaction of RssB with σS. We found that the anti-adaptor protein IraD has higher affinity than σS to RssB and its binding interface on RssB overlaps with that for σS. And IraD-RssB complex is preferred over RssB-σS in solution, regardless of the phosphorylation state of RssB. Our study suggests that RssB possesses a two-tier mechanism for regulating σS. First, phosphorylation of RssB provides a moderate and reversible tempering of its activity, followed by a specific and robust inhibition via the anti-adaptor interaction.

Keyword :

sigma S phosphorylation RssB anti-adaptor protein transcription regulation

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GB/T 7714 Wang Zhihao , Zhao Siyu , Li Yanqing et al. RssB-mediated σS Activation is Regulated by a Two-Tier Mechanism via Phosphorylation and Adaptor Protein - IraD. [J]. | Journal of molecular biology , 2021 , 433 (3) .
MLA Wang Zhihao et al. "RssB-mediated σS Activation is Regulated by a Two-Tier Mechanism via Phosphorylation and Adaptor Protein - IraD." . | Journal of molecular biology 433 . 3 (2021) .
APA Wang Zhihao , Zhao Siyu , Li Yanqing , Zhang Kaining , Mo Fei , Zhang Jiye et al. RssB-mediated σS Activation is Regulated by a Two-Tier Mechanism via Phosphorylation and Adaptor Protein - IraD. . | Journal of molecular biology , 2021 , 433 (3) .
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Novel bioavailability-based risk assessment of Cd in earthworms and leeches utilizing in vitro digestion/Caco-2 and MDCK cells SCIE PubMed
期刊论文 | 2021 | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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In the present study, the oral bioavailability of cadmium (Cd) in earthworms and leeches was investigated through in vitro physiologically based extraction test (PBET) digestion/Caco2 and MDKC cell models. We are the first to create an innovative assessment strategy which has capacity to offer a more precise evaluation of Cd-associated health risks in traditional animal medicines (TAMs), by combinational usage of bioavailable Cd levels, the duration and frequency of the exposure to TAMs obtained by questionnaire data, as well as safety factor of TAMs. Our data showed that the percentage of bioavailability for Caco-2 cells in earthworms and leeches ranged from 3.29 to 14.17% and 4.32 to 12.61%, respectively. The percentage of bioavailability of MDCK cells in earthworms and leeches ranged from 4.83 to 15.74% and 6.53 to 15.04%, respectively. After adjusting by the bioavailability of Cd to target hazard quotient (THQ), excitingly, our findings manifested that the health risks induced by the ingestion of earthworms and leeches were acceptable in the clinic. Our key findings suggest that bioavailability characterization cannot be ruled out and health risks should be assessed on the basis of the bioavailable Cd levels rather than total levels. Our novel strategy provides insight into the bio-accumulation of Cd in organisms as well as a more realistic and accurate assessment of Cd-associated health risks in TAMs, with the main purpose of improving public health by scientifically using TAMs.

Keyword :

Cd In vitro digestion Risk assessment Traditional animal medicines MDCK cell model Bioavailability Caco-2 cell model

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GB/T 7714 Zuo, Tian-tian , Luo, Fei-ya , He, Huai-zhen et al. Novel bioavailability-based risk assessment of Cd in earthworms and leeches utilizing in vitro digestion/Caco-2 and MDCK cells [J]. | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH , 2021 .
MLA Zuo, Tian-tian et al. "Novel bioavailability-based risk assessment of Cd in earthworms and leeches utilizing in vitro digestion/Caco-2 and MDCK cells" . | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) .
APA Zuo, Tian-tian , Luo, Fei-ya , He, Huai-zhen , Jin, Hong-yu , Sun, Lei , Xing, Shu-xia et al. Novel bioavailability-based risk assessment of Cd in earthworms and leeches utilizing in vitro digestion/Caco-2 and MDCK cells . | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH , 2021 .
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Substance P-induced lung inflammation in mice is mast cell dependent SCIE PubMed
期刊论文 | 2021 | CLINICAL AND EXPERIMENTAL ALLERGY
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Background Allergic asthma is a common inflammatory lung disease and a major health problem worldwide. Mast cells (MCs) play a key role in the early-stage pathophysiology of allergic asthma. Substance P (SP) functions in neurogenic inflammation by activating MCs, and therefore, it may to participate in the occurrence and development of asthma. Objective We examined the relationship between SP and lung inflammation, and also whether SP can directly trigger asthma. Methods We measured the number of peripheral blood eosinophils, neutrophils and basophils and evaluated the levels of IgE and SP in blood samples of 86 individuals with allergic asthma. Serum IgE and SP levels were also determined in 29 healthy individuals. C57BL/6 mice were subjected to different doses of SP, and bronchoalveolar lavage fluid (BALF) was collected to count the inflammatory cells. Lung tissues were analysed using histopathological methods to evaluate lung peribronchial inflammation, fibrosis and glycogen deposition. Levels of IgE, interleukin (IL)-1, IL-2, IL-4, IL-5, IL-13, IL-17 and IFN-gamma were determined in mouse serum. Results Substance P levels were increased in the serum samples of patients with asthma. SP induced mouse lung peribronchial inflammation, fibrosis and glycogen deposition, with high levels of Th2-related cytokines such as IL-4, IL-5 and IL-13 observed in the BALF. Furthermore, low level of total IgE was noted in the serum, and SP had little effect on MC-deficient kit(W-sh/W-sh) mice. Conclusions & clinical relevance Substance P levels increased significantly in serum of asthmatic patients and independently associated with the risk of asthma. Furthermore, SP induced Th2 lung inflammation in mice, which was dependent on MCs.

Keyword :

Th2 mast cell asthma SP

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GB/T 7714 Wang, Nan , Wang, Jue , Zhang, Yongjing et al. Substance P-induced lung inflammation in mice is mast cell dependent [J]. | CLINICAL AND EXPERIMENTAL ALLERGY , 2021 .
MLA Wang, Nan et al. "Substance P-induced lung inflammation in mice is mast cell dependent" . | CLINICAL AND EXPERIMENTAL ALLERGY (2021) .
APA Wang, Nan , Wang, Jue , Zhang, Yongjing , Hu, Shiling , Zhang, Tianxiao , Wu, Yuanyuan et al. Substance P-induced lung inflammation in mice is mast cell dependent . | CLINICAL AND EXPERIMENTAL ALLERGY , 2021 .
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Establishment of substance P modified affinity chromatography for specific detection and enrichment of Mas-related G protein–coupled receptor X2 EI SCIE PubMed
期刊论文 | 2021 , 1659 | Journal of Chromatography A
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Mas-related G protein-coupled receptor X2 (MrgX2) has been identified to be critical in drug-induced pseudo-allergic reactions and allergic diseases. Herein, an affinity high-performance liquid chromatography was established for the specific detection and enrichment of MrgX2. Substance P was used as an affinity ligand and immobilized on a glutaraldehyde-modified amino silica gel. The successful grafting of substance P was characterized by infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, thermogravimetric analysis, and nitrogen adsorption and desorption analyzes. The prepared materials were then used as the stationary phase to investigate the retention behavior of MrgX2 recombinant protein on the affinity column. The results obtained with the analytical techniques show the specificity and selectivity of the MrgX2 recombinant protein on the affinity column. The repeatability and reproducibility for the analysis of MrgX2 on the NH2-Silico@GD@SP column show relative standard deviation (RSD) values lower than the acceptance criteria of 2 and 5% of retention time, and RSD of peak areas 2-Silico@GD@SP column offline SEC system has been tested by using MrgX2 as positive control. The results of this experiment indicate that the offline system may be used to analyze the retention fraction. MrgX2 extracted from human mast cells LAD2 was also verified. An obvious retention can be observed and the natural MrgX2 was concentrated 114.6 times compared with the original complex components by using the affinity column. These results may provide a new approach for the specific detection and enrichment of G-protein-coupled receptors. © 2021 Elsevier B.V.

Keyword :

Affinity chromatography Infrared spectroscopy Mass spectrometry Thermogravimetric analysis High performance liquid chromatography Controlled drug delivery Silica gel X ray photoelectron spectroscopy Recombinant proteins Gas adsorption

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GB/T 7714 Hou, Yajing , Lu, Jiayu , Wei, Di et al. Establishment of substance P modified affinity chromatography for specific detection and enrichment of Mas-related G protein–coupled receptor X2 [J]. | Journal of Chromatography A , 2021 , 1659 .
MLA Hou, Yajing et al. "Establishment of substance P modified affinity chromatography for specific detection and enrichment of Mas-related G protein–coupled receptor X2" . | Journal of Chromatography A 1659 (2021) .
APA Hou, Yajing , Lu, Jiayu , Wei, Di , Lv, Yuexin , He, Huaizhen , Wang, Cheng et al. Establishment of substance P modified affinity chromatography for specific detection and enrichment of Mas-related G protein–coupled receptor X2 . | Journal of Chromatography A , 2021 , 1659 .
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Screening and evaluation of anti-SARS-CoV-2 components from Ephedra sinica by ACE2/CMC-HPLC-IT-TOF-MS approach EI SCIE PubMed
期刊论文 | 2021 , 413 (11) , 2995-3004 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
WoS CC Cited Count: 6
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Traditional Chinese medicines played an important role in the treatment of COVID-19 in 2020. Ephedra sinica, one of the major constituent herbs of multi-component herbal formula, has been widely used to treat COVID-19 in China. However, its active components are still unclear. The objectives of this study are to screen and evaluate active components from the traditional Chinese medicine Ephedra sinica for the treatment of COVID-19. In our study, we established an ACE2/CMC bioaffinity chromatography model, and then developed an ACE2/CMC-HPLC-IT-TOF-MS system for the active compounds screening and identification from Ephedra sinica extract. We performed molecular docking and surface plasmon resonance (SPR) assays to assess the binding characteristics (binding mode and K-D value). We used CCK-8 staining to assess the toxicity of screened compounds, and also used SARS-CoV-2 pseudovirus to observe the viropexis effect of screened compounds in ACE2(h) cells. In this current work, one fraction was fished out, separated and identified as ephedrine (EP), pseudoephedrine (PEP), and methylephedrine (MEP). Binding assays showed that the three compounds could bind with ACE2 in a special way to some amino acid residues, similar to the way SARS-CoV-2 bound with ACE2. Additionally, the three compounds, especially EP, can inhibit the entrance of SARS-CoV-2 spike pseudovirus into ACE2(h) cells because they can reduce the entrance ratio of pseudovirus in the pseudovirus model. Overall, the ACE2/CMC-HPLC-IT-TOF-MS system was established and verified to be suitable for ACE2-targeted bioactive compound screening. EP, PEP, and MEP with ACE2-binding features were screened out from Ephedra sinica, and acted as blockers inhibiting SARS-CoV-2 spike pseudovirus entering ACE2(h) cells.

Keyword :

Ephedra sinica Cell membrane chromatography ACE2 receptor COVID-19

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GB/T 7714 Lv, Yanni , Wang, Saisai , Liang, Peida et al. Screening and evaluation of anti-SARS-CoV-2 components from Ephedra sinica by ACE2/CMC-HPLC-IT-TOF-MS approach [J]. | ANALYTICAL AND BIOANALYTICAL CHEMISTRY , 2021 , 413 (11) : 2995-3004 .
MLA Lv, Yanni et al. "Screening and evaluation of anti-SARS-CoV-2 components from Ephedra sinica by ACE2/CMC-HPLC-IT-TOF-MS approach" . | ANALYTICAL AND BIOANALYTICAL CHEMISTRY 413 . 11 (2021) : 2995-3004 .
APA Lv, Yanni , Wang, Saisai , Liang, Peida , Wang, Yamin , Zhang, Xin , Jia, Qianqian et al. Screening and evaluation of anti-SARS-CoV-2 components from Ephedra sinica by ACE2/CMC-HPLC-IT-TOF-MS approach . | ANALYTICAL AND BIOANALYTICAL CHEMISTRY , 2021 , 413 (11) , 2995-3004 .
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