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学者姓名：贺健康
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Abstract ：
Y YThe pathological research and drug development of brain diseases require appropriate brain models. Given the complex, layered structure of the cerebral cortex, as well as the constraints on the medical ethics and the inaccuracy of animal models, it is necessary to construct a brain-like model in vitro. In this study, we designed and built integrated three-dimensional (3D) printing equipment for cell printing/culture, which can guarantee cell viability in the printing process and provide the equipment foundation for manufacturing the layered structures with gradient distribution of pore size. Based on this printing equipment, to achieve the purpose of printing the layered structures with multiple materials, we conducted research on the performance of bio-inks with different compositions and optimized the printing process. By extruding and stacking materials, we can print the layered structure with the uniform distribution of cells and the gradient distribution of pore sizes. Finally, we can accurately print a structure with 30 layers. The line width (resolution) of the printed monolayer structure was about 478 mu m, the forming accuracy can reach 97.24%, and the viability of cells in the printed structure is as high as 94.5%.
Keyword ：
Integrated cell printing/culture equipment 3D bio-printing Layered gradient structure Brain-like model
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| GB/T 7714 | Pei, Na , Hao, Zhiyan , Wang, Sen et al. 3D Printing of Layered Gradient Pore Structure of Brain-like Tissue [J]. | INTERNATIONAL JOURNAL OF BIOPRINTING , 2021 , 7 (3) : 71-85 . |
| MLA | Pei, Na et al. "3D Printing of Layered Gradient Pore Structure of Brain-like Tissue" . | INTERNATIONAL JOURNAL OF BIOPRINTING 7 . 3 (2021) : 71-85 . |
| APA | Pei, Na , Hao, Zhiyan , Wang, Sen , Pan, Binglei , Fang, Ao , Kang, Jianfeng et al. 3D Printing of Layered Gradient Pore Structure of Brain-like Tissue . | INTERNATIONAL JOURNAL OF BIOPRINTING , 2021 , 7 (3) , 71-85 . |
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Abstract ：
3D printed external airway splints derived from degradable biopolymers provide a promising way to treat tracheomalacia (TM). However, most of the splints cannot fully reproduce the lumen shapes and the mechanical properties of native tracheas. In this study, biodegradable external airway splints with native-like lumen geometry and mechanical properties were designed and fabricated. Matchable mechanical properties were realized by the combination of different pore shapes and sizes of the splints. A reliable method for the fabrication of the porous polycaprolactone (PCL) splints was demonstrated via selective laser sintering (SLS). Compression results revealed that the SLS-fabricated PCL splints with the diamond pore shape and an inscribed circle diameter of 3 mm possessed similar mechanical properties to those of native tracheas, before and after implantation in dogs with TM for 12 weeks. These SLSfabricated PCL biodegradable bionic external airway splints might be good candidate for clinical application in future. (c) 2021 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keyword ：
Selective laser sintering (SLS) Tracheomalacia (TM) 3-dimensional printed (3DP) External airway splint Polycaprolactone (PCL)
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| GB/T 7714 | Liu, Wenhao , Meng, Zijie , Zheng, Kaifu et al. Development of three-dimensional printed biodegradable external airway splints with native-like shape and mechanical properties for tracheomalacia treatment [J]. | MATERIALS & DESIGN , 2021 , 210 . |
| MLA | Liu, Wenhao et al. "Development of three-dimensional printed biodegradable external airway splints with native-like shape and mechanical properties for tracheomalacia treatment" . | MATERIALS & DESIGN 210 (2021) . |
| APA | Liu, Wenhao , Meng, Zijie , Zheng, Kaifu , Wang, Lei , Zhang, Chenxi , Ji, Jinjie et al. Development of three-dimensional printed biodegradable external airway splints with native-like shape and mechanical properties for tracheomalacia treatment . | MATERIALS & DESIGN , 2021 , 210 . |
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Abstract ：
Helical architectures were designed for the additive manufacturing of highly flexible polycaprolactone (PCL) scaffolds for engineering soft tissues, which commonly require high flexibility and predominantly function under large deformation conditions. It was found that the design parameters like revolution and radius of helical architectures highly affected the filament fusion or bonding during the fabrication process. The bonding-induced interlaced helical architectures resulted in a more uniform deformation pattern and a lower modulus. mu CT-based finite element method was established to predict the large deformation responses of the scaffolds with helical architectures and patient-specific scaffold potentially for breast reconstruction, which showed well agreement with the experimental results. The fabricated scaffolds exhibited good shape recovery capability even under cyclical compression at a strain of about 20% for 10000 times. This exploration offers a promising way to predict the mechanical responses of flexible scaffolds with complex helical architectures under large deformation conditions.
Keyword ：
large deformation response Additive manufacturing helical architectures breast tissue engineering
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| GB/T 7714 | Meng, Zijie , He, Jiankang , Li, Dichen . Additive manufacturing and large deformation responses of highly-porous polycaprolactone scaffolds with helical architectures for breast tissue engineering [J]. | VIRTUAL AND PHYSICAL PROTOTYPING , 2021 , 16 (3) : 291-305 . |
| MLA | Meng, Zijie et al. "Additive manufacturing and large deformation responses of highly-porous polycaprolactone scaffolds with helical architectures for breast tissue engineering" . | VIRTUAL AND PHYSICAL PROTOTYPING 16 . 3 (2021) : 291-305 . |
| APA | Meng, Zijie , He, Jiankang , Li, Dichen . Additive manufacturing and large deformation responses of highly-porous polycaprolactone scaffolds with helical architectures for breast tissue engineering . | VIRTUAL AND PHYSICAL PROTOTYPING , 2021 , 16 (3) , 291-305 . |
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Implantable nerve electrodes, as a bridge between the brain and external devices, have been widely used in areas such as brain function exploration, neurological disease treatment and human-computer interaction. However, the mechanical properties mismatch between the electrode material and the brain tissue seriously affects the stability of electrode signal acquisition and the effectiveness of long-term service in vivo. In this study, a modified neuroelectrode was developed with conductive biomaterials. The electrode has good biocompatibility and a gradient microstructure suitable for cell growth. Compared with metal electrodes, bioelectrodes not only greatly reduced the elastic modulus (<10 kpa) but also increased the conductivity of the electrode by 200 times. Through acute electrophysiological analysis and a 12-week chronic in vivo experiment, the bioelectrode clearly recorded the rat's brain electrical signals, effectively avoided the generation of glial scars and induced neurons to move closer to the electrode. The new conductive biomaterial electrodes developed in this research make long-term implantation of cortical nerve electrodes possible.
Keyword ：
polyaniline glial response conductive biomaterial neural electrode
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| GB/T 7714 | Hao, Zhiyan , Wang, Sen , Zhang, Kun et al. Biofabrication of a Low Modulus Bioelectroprobe for Neurons to Grow Into [J]. | MATERIALS , 2021 , 14 (16) . |
| MLA | Hao, Zhiyan et al. "Biofabrication of a Low Modulus Bioelectroprobe for Neurons to Grow Into" . | MATERIALS 14 . 16 (2021) . |
| APA | Hao, Zhiyan , Wang, Sen , Zhang, Kun , Zhou, Jiajia , Li, Dichen , He, Jiankang et al. Biofabrication of a Low Modulus Bioelectroprobe for Neurons to Grow Into . | MATERIALS , 2021 , 14 (16) . |
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Abstract ：
Electrohydrodynamic (EHD) 3D printing of carbon-based materials in the form of orderly networks can have various applications. In this work, microscale carbon/nickel (C-Ni) composite electrodes with controlled porosity have been utilized in electrochemical energy storage of supercapacitors. Polyacrylonitrile (PAN) was chosen as the basic material for its excellent carbonization performance and EHD printing property. Nickel nitrate (Ni(NO3)2) was incorporated to form Ni nanoparticles which can improve the conductivity and the capacitance performance of the electrode. Well-aligned PAN-Ni(NO3)2 composite structures have been fabricated and carbonized as C-Ni electrodes with the typical diameter of 9.2±2.1 μm. The porosity of the as-prepared C-Ni electrode can be controlled during the EHD process. Electrochemical results show the C-Ni network electrode has achieved a 2.3 times higher areal specific capacitance and 1.7 times higher mass specific capacitance than those of a spin-coated electrode. As such, this process offers a facile and scalable strategy for the fabrication of orderly carbon-based conductive structures for various applications such as energy storage devices and printable electronics. © 2021
Keyword ：
Porosity 3D printers Carbon Coated wire electrodes Capacitance Energy storage Process control Composite structures Nickel compounds Electrohydrodynamics Supercapacitor Carbonization
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| GB/T 7714 | Zhang, Bing , He, Jiankang , Zheng, Gaofeng et al. Electrohydrodynamic 3D printing of orderly carbon/nickel composite network as supercapacitor electrodes [J]. | Journal of Materials Science and Technology , 2021 , 82 : 135-143 . |
| MLA | Zhang, Bing et al. "Electrohydrodynamic 3D printing of orderly carbon/nickel composite network as supercapacitor electrodes" . | Journal of Materials Science and Technology 82 (2021) : 135-143 . |
| APA | Zhang, Bing , He, Jiankang , Zheng, Gaofeng , Huang, Yuanyuan , Wang, Chaohung , He, Peisheng et al. Electrohydrodynamic 3D printing of orderly carbon/nickel composite network as supercapacitor electrodes . | Journal of Materials Science and Technology , 2021 , 82 , 135-143 . |
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| GB/T 7714 | He, Jiankang , Mao, Mao , Li, Xiao et al. Bioprinting of 3D Functional Tissue Constructs [J]. | INTERNATIONAL JOURNAL OF BIOPRINTING , 2021 , 7 (3) : 1-2 . |
| MLA | He, Jiankang et al. "Bioprinting of 3D Functional Tissue Constructs" . | INTERNATIONAL JOURNAL OF BIOPRINTING 7 . 3 (2021) : 1-2 . |
| APA | He, Jiankang , Mao, Mao , Li, Xiao , Chua, Chee Kai . Bioprinting of 3D Functional Tissue Constructs . | INTERNATIONAL JOURNAL OF BIOPRINTING , 2021 , 7 (3) , 1-2 . |
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Abstract ：
Recapitulating the vascular networks that maintain the delivery of nutrition, oxygen, and byproducts for the living cells within the three-dimensional (3D) tissue constructs is a challenging issue in the tissue-engineering area. Here, a novel coaxial electrohydrodynamic (EHD) bioprinting strategy is presented to fabricate thick pre-vascularized cell-laden constructs. The alginate and collagen/calcium chloride solution were utilized as the outer-layer and inner-layer bioink, respectively, in the coaxial printing nozzle to produce the core-sheath hydrogel filaments. The effect of process parameters (the feeding rate of alginate and collagen and the moving speed of the printing stage) on the size of core and sheath lines within the printed filaments was investigated. The core-sheath filaments were printed in the predefined pattern to fabricate lattice hydrogel with perfusable lumen structures. Endothelialized lumen structures were fabricated by culturing the core-sheath filaments with endothelial cells laden in the core collagen hydrogel. Multilayer core-sheath filaments were successfully printed into 3D porous hydrogel constructs with a thickness of more than 3 mm. Finally, 3D pre-vascularized cardiac constructs were successfully generated, indicating the efficacy of our strategy to engineer living tissues with complex vascular structures.
Keyword ：
Biofabrication Coaxial bioprinting Core-sheath filaments Electrohydrodynamic bioprinting Vascularized tissues
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| GB/T 7714 | Mao, Mao , Liang, Hongtao , He, Jiankang et al. Coaxial Electrohydrodynamic Bioprinting of Pre-vascularized Cell-laden Constructs for Tissue Engineering [J]. | INTERNATIONAL JOURNAL OF BIOPRINTING , 2021 , 7 (3) : 86-96 . |
| MLA | Mao, Mao et al. "Coaxial Electrohydrodynamic Bioprinting of Pre-vascularized Cell-laden Constructs for Tissue Engineering" . | INTERNATIONAL JOURNAL OF BIOPRINTING 7 . 3 (2021) : 86-96 . |
| APA | Mao, Mao , Liang, Hongtao , He, Jiankang , Kasimu, Ayiguli , Zhang, Yanning , Wang, Ling et al. Coaxial Electrohydrodynamic Bioprinting of Pre-vascularized Cell-laden Constructs for Tissue Engineering . | INTERNATIONAL JOURNAL OF BIOPRINTING , 2021 , 7 (3) , 86-96 . |
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Nanofibrous scaffolds with structural and compositional gradients exhibit great potential to modulate zonal differentiation of stem cells for the regeneration of soft-to-hard tissue interface. Here, the response of bone marrow stem cells (BMSCs) to electrospun gradient nanofibrous scaffolds was investigated to demonstrate their potential capabilities for interfacial tissue regeneration. The electrospun scaffolds showed gradient distribution of BMP-2/nanoHA contents and the fiber orientations gradually changed from random to align. Biomimetic mineralization demonstrated that calcium and phosphorus elements can deposit onto the surface of the nanofibers in a gradient manner similar to nanoHA content. BMSCs cultured on the gradient nanofibrous scaffolds exhibited high cell viability and cell morphology gradually changed from disorder to highly align similar to the underlying fiber orientation. BMP-2/nanoHA content gradients in the nanofibrous scaffolds were found to effectively promote the zonal expression of bone-specific genes like osteocalcin (OCN), Runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP). Immunofluorescent staining of osteopontin (OPN) and OCN further confirmed osteoblastic phenotypic maturation on the regions of the scaffolds with a higher level of nanoHA and BMP-2 contents after cultured 28 days. These results indicated that the gradient nanofibrous scaffolds enable to guide zonal differentiation of BMSCs in vitro, which might be useful to realize multitissue regeneration in one construct for the regeneration of soft-to-hard tissue interface. © 2020 Elsevier B.V.
Keyword ：
Biomimetics Scaffolds (biology) Bone Stem cells Cytology Morphology Tissue engineering Tissue regeneration Nanofibers Transcription Phosphatases Electrospinning Tissue
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| GB/T 7714 | Jiang, Nan , He, Jiankang , Zhang, Weijie et al. Directed differentiation of BMSCs on structural/compositional gradient nanofibrous scaffolds for ligament-bone osteointegration [J]. | Materials Science and Engineering C , 2020 , 110 . |
| MLA | Jiang, Nan et al. "Directed differentiation of BMSCs on structural/compositional gradient nanofibrous scaffolds for ligament-bone osteointegration" . | Materials Science and Engineering C 110 (2020) . |
| APA | Jiang, Nan , He, Jiankang , Zhang, Weijie , Li, Dichen , Lv, Yi . Directed differentiation of BMSCs on structural/compositional gradient nanofibrous scaffolds for ligament-bone osteointegration . | Materials Science and Engineering C , 2020 , 110 . |
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Abstract ：
We emulated instances of open traumatic brain injuries (FBI) in a maritime disaster. New Zealand rabbit animal models were used to evaluate the pathophysiological changes in open TBI with and without the influence of artificial seawater. New Zealand rabbits were randomly divided into 3 groups. Control group consisted of only normal animals. Animals in TBI and TBI + Seawater groups underwent craniotomy with dura mater incised and brain tissue exposed to free-fall impact. Afterward, only TBI + Seawater group received on-site artificial seawater infusion. Brain water content (BWC) and permeability of blood-brain barrier (BBB) were assessed. Reactive oxygen species levels were measured. Western blotting and immunofluorescence were employed to detect: apoptosis-related factors Caspase-3, Bax and Bcl-2; angiogenesis-related factors CD31 and CD34; astrogliosis-related factor glial fibrillary acidic protein (GFAP); potential neuron injury indicator neuron-specific enolase (NSE). Hematoxylin & eosin, Masson-trichrome and Nissl stainings were performed for pathological observations. Comparing to Control group, TBI group manifested abnormal neuronal morphology; increased BWC; compromised BBB integrity; increased ROS, Bax, CD31, CD34, Caspase-3 and GFAP expressions; decreased Bcl-2 and NSE expression. Seawater immersion caused all changes, except BWC, to become more significant. Seawater immersion worsens the damage inflicted to brain tissue by open TBI. It aggravates hypoxia in brain tissue, upregulates ROS expression, increases neuron sensitivity to apoptosis-inducing factors, and promotes angiogenesis as well as astrogliosis.
Keyword ：
Seawater Blood-brain barrier Open Traumatic Brain Injury Apoptosis Pathophysiology
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| GB/T 7714 | Liu, Kai-Li , Yu, Xiao-Jing , Sun, Tian-Ze et al. Effects of seawater immersion on open traumatic brain injury in rabbit model [J]. | BRAIN RESEARCH , 2020 , 1743 . |
| MLA | Liu, Kai-Li et al. "Effects of seawater immersion on open traumatic brain injury in rabbit model" . | BRAIN RESEARCH 1743 (2020) . |
| APA | Liu, Kai-Li , Yu, Xiao-Jing , Sun, Tian-Ze , Wang, Yi-Chang , Chen, Meng-Xuan , Su, Yan-Wen et al. Effects of seawater immersion on open traumatic brain injury in rabbit model . | BRAIN RESEARCH , 2020 , 1743 . |
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Abstract ：
Additively-manufactured PEEK orthopedic implants have recently gained extensive attention due to their prominent characteristics such as good biocompatibility, low radiographic artifacts and similar elastic modulus to native bones. However, the inherent drawback associated with PEEK implants was their biologically inert surface which caused unsatisfactory cellular response and poor adhesion between the implants and surrounding soft tissues. Here we developed a sulfonation-treatment strategy to create microporous architectures onto the filaments of the additively-manufactured PEEK lattice scaffolds. The sulfonation time in the range of 30-45 s was found to facilitate the formation of uniform microscale pores throughout the printed PEEK lattice scaffolds and simultaneously have slight effect on their composition and mechanical properties. Biological results showed that the presence of microscale pores on the additively-manufactured PEEK lattice scaffolds significantly improved the spreading, proliferation and calcium deposition of bone-specific cells in comparison with the untreated PEEK lattice scaffolds. In vivo experiments demonstrated that the sulfonation-treated micropores facilitated the adhesion of newly-regenerated soft tissues to form tight implant-tissue bonding interfaces. The presented method provides a promising approach to improve the surface bioactivity of additively-manufactured PEEK lattice scaffolds for enhanced cellular response and soft tissue adhesion. (C) 2020 The Authors. Published by Elsevier Ltd.
Keyword ：
Microporous architectures Bioactivity Scaffold Sulfonation Additive manufacturing Polyetheretherketone
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| GB/T 7714 | Su, Yanwen , He, Jiankang , Jiang, Nan et al. Additively-manufactured poly-ether-ether-ketone (PEEK) lattice scaffolds with uniform microporous architectures for enhanced cellular response and soft tissue adhesion [J]. | MATERIALS & DESIGN , 2020 , 191 . |
| MLA | Su, Yanwen et al. "Additively-manufactured poly-ether-ether-ketone (PEEK) lattice scaffolds with uniform microporous architectures for enhanced cellular response and soft tissue adhesion" . | MATERIALS & DESIGN 191 (2020) . |
| APA | Su, Yanwen , He, Jiankang , Jiang, Nan , Zhang, Hao , Wang, Lei , Liu, Xi et al. Additively-manufactured poly-ether-ether-ketone (PEEK) lattice scaffolds with uniform microporous architectures for enhanced cellular response and soft tissue adhesion . | MATERIALS & DESIGN , 2020 , 191 . |
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