• Complex
  • Title
  • Author
  • Keyword
  • Abstract
  • Scholars
Search
High Impact Results & Cited Count Trend for Year Keyword Cloud and Partner Relationship

Query:

学者姓名:徐峰

Refining:

Source

Submit Unfold

Co-Author

Submit Unfold

Language

Submit

Clean All

Export Sort by:
Default
  • Default
  • Title
  • Year
  • WOS Cited Count
  • Impact factor
  • Ascending
  • Descending
< Page ,Total 43 >
Effect of gene mutation of plants on their mechano-sensibility: the mutant of EXO70H4 influences the buckling of Arabidopsis trichomes EI SCIE
期刊论文 | 2021 , 146 (16) , 5169-5176 | ANALYST
Abstract&Keyword Cite

Abstract :

With the development of molecular biology, more and more mutants of plants have been constructed, where gene mutants have been found to influence not only the biological processes but also biophysical behaviors of plant cells. Trichomes are an important appendage, which has been found to act as an active mechanosensory switch transducing mechanical signals into physiology changes, where the mechanical property of trichomes is vital for such functions. Up to now, over 40 different genes have been found with the function of regulating trichome cell morphogenesis; however, the effect of gene mutants on trichome mechanosensory function remains elusive. In this study, we found that EXO70H4, one of the most up-regulated genes in the mature trichome, not only affects the thickness of the trichome cell wall but also the mechanical property (i.e., the Young's modulus) of trichomes. Finite element method simulation results show that the buckling instability and stress concentration (e.g., exerted by insects) cannot occur on the base of the mutant exo70H4 trichome, which might further interrupt the mechanical signal transduction from branches to the base of trichomes. These results indicated that the mutant exo70H4 trichome might lack the ability to act as an active mechanosensory switch against chewing insect herbivores. Our findings provide new information about the effect of gene mutation (like crop mutants) on the mechano-sensibility and capability to resist the agricultural pests or lodging, which could be of great significance to the development of agriculture.

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Liu, Han , Liu, Shaobao , Huang, Guoyou et al. Effect of gene mutation of plants on their mechano-sensibility: the mutant of EXO70H4 influences the buckling of Arabidopsis trichomes [J]. | ANALYST , 2021 , 146 (16) : 5169-5176 .
MLA Liu, Han et al. "Effect of gene mutation of plants on their mechano-sensibility: the mutant of EXO70H4 influences the buckling of Arabidopsis trichomes" . | ANALYST 146 . 16 (2021) : 5169-5176 .
APA Liu, Han , Liu, Shaobao , Huang, Guoyou , Xu, Feng . Effect of gene mutation of plants on their mechano-sensibility: the mutant of EXO70H4 influences the buckling of Arabidopsis trichomes . | ANALYST , 2021 , 146 (16) , 5169-5176 .
Export to NoteExpress RIS BibTex
Cancer Physical Hallmarks as New Targets for Improved Immunotherapy SCIE PubMed
期刊论文 | 2021 , 31 (7) , 520-524 | TRENDS IN CELL BIOLOGY
Abstract&Keyword Cite

Abstract :

A low response rate is the major challenge for existing cancer immunotherapies. Physical cues in the tumor microenvironment (TME) have been recognized as new hallmarks of cancer, which may synergistically contribute to low immunotherapy response. Recent evidence indicates that the physical hallmarks of cancer hold great potential as new targets for improved immunotherapy.

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Guo, Hui , Zhang, Tian , Yu, Yang et al. Cancer Physical Hallmarks as New Targets for Improved Immunotherapy [J]. | TRENDS IN CELL BIOLOGY , 2021 , 31 (7) : 520-524 .
MLA Guo, Hui et al. "Cancer Physical Hallmarks as New Targets for Improved Immunotherapy" . | TRENDS IN CELL BIOLOGY 31 . 7 (2021) : 520-524 .
APA Guo, Hui , Zhang, Tian , Yu, Yang , Xu, Feng . Cancer Physical Hallmarks as New Targets for Improved Immunotherapy . | TRENDS IN CELL BIOLOGY , 2021 , 31 (7) , 520-524 .
Export to NoteExpress RIS BibTex
Taqman-MGB nanoPCR for Highly Specific Detection of Single-Base Mutations SCIE PubMed
期刊论文 | 2021 , 16 , 3695-3705 | INTERNATIONAL JOURNAL OF NANOMEDICINE
WoS CC Cited Count: 1
Abstract&Keyword Cite

Abstract :

Purpose: Detection of single-base mutations is important for real-time monitoring of tumor progression, therapeutic effects, and drug resistance. However, the specific detection of single-base mutations from excessive wild-type background sequences with routine PCR technology remains challenging. Our objective is to develop a simple and highly specific qPCR-based single-base mutation detection method. Methods: Using EGRF T790M as a model, gold nanoparticles at different concentrations were separately added into the Taqman-MGB qPCR system to test specificity improvement, leading to the development of the optimal Taqman-MGB nanoPCR system. Then, these optimal conditions were used to test the range of improvement in the specificity of mutant-type and wild-type templates and the detection limit of mutation abundances in a spiked sample. Results: The Taqman-MGB nanoPCR was established based on the traditional qPCR, with significantly suppressed background noise and improved specificity for single-base mutation detection. With EGFR T790M as a template, we demonstrated that our Taqman-MGB nanoPCR system could improve specificity across a wide concentration range from 10(-9) mu M to 10 mu M and detect as low as 0.95% mutation abundance in spiked samples, which is lower than what the traditional Taqman-MGB qPCR and existing PCR methods can detect. Moreover, we also proposed an experimentally validated barrier hypothesis for the mechanism of improved specificity. Conclusion: The developed Taqman-MGB nanoPCR system could be a powerful tool for clinical single-base mutation detection.

Keyword :

gold nanoparticle specificity nanoparticle-assisted PCR point mutation

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Xue, Zhenrui , You, Minli , Peng, Ping et al. Taqman-MGB nanoPCR for Highly Specific Detection of Single-Base Mutations [J]. | INTERNATIONAL JOURNAL OF NANOMEDICINE , 2021 , 16 : 3695-3705 .
MLA Xue, Zhenrui et al. "Taqman-MGB nanoPCR for Highly Specific Detection of Single-Base Mutations" . | INTERNATIONAL JOURNAL OF NANOMEDICINE 16 (2021) : 3695-3705 .
APA Xue, Zhenrui , You, Minli , Peng, Ping , Tong, Haoyang , He, Wanghong , Li, Ang et al. Taqman-MGB nanoPCR for Highly Specific Detection of Single-Base Mutations . | INTERNATIONAL JOURNAL OF NANOMEDICINE , 2021 , 16 , 3695-3705 .
Export to NoteExpress RIS BibTex
考虑细胞外基质黏弹性行为的细胞黏附力学模型 CSCD
期刊论文 | 2021 , 42 (10) , 1074-1080 | 应用数学和力学
Abstract&Keyword Cite

Abstract :

细胞外基质由大量胶原蛋白和纤维蛋白组成,这些基质蛋白形成复杂的交联网络状结构,具有黏弹性力学特性.研究表明,黏弹性基质能显著影响细胞迁移、增殖和分化等生理行为,还能影响癌症转移和组织纤维化等疾病的发生与发展.然而,细胞感知细胞外基质黏弹性力学特性的分子机制仍不清楚.该文通过建立细胞黏附力学模型,从分子层次揭示细胞黏附在细胞响应外界黏弹性力学微环境中的作用.结果表明,细胞能通过调控细胞黏附动力学(包括黏附周期和黏附形成时间)响应细胞外基质的黏弹性力学特性.通过将模型计算结果与实验现象相比较,验证了模型的正确性.细胞黏附力学模型将为组织工程中细胞力学微环境的构建奠定理论基础.

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 程波 , 徐峰 . 考虑细胞外基质黏弹性行为的细胞黏附力学模型 [J]. | 应用数学和力学 , 2021 , 42 (10) : 1074-1080 .
MLA 程波 et al. "考虑细胞外基质黏弹性行为的细胞黏附力学模型" . | 应用数学和力学 42 . 10 (2021) : 1074-1080 .
APA 程波 , 徐峰 . 考虑细胞外基质黏弹性行为的细胞黏附力学模型 . | 应用数学和力学 , 2021 , 42 (10) , 1074-1080 .
Export to NoteExpress RIS BibTex
Three-dimensional mechanical microenvironment enhanced osteogenic activity of mesenchymal stem cells-derived exosomes EI SCIE
期刊论文 | 2021 , 417 | CHEMICAL ENGINEERING JOURNAL
WoS CC Cited Count: 1
Abstract&Keyword Cite

Abstract :

Exosomes derived from mesenchymal stem cells (MSCs) through the paracrine pathway have shown great potentials in cell-free therapy for regenerative medicine. Although MSCs have been recently known to respond to the mechanical microenvironment and thus orchestrate their behaviors and functions, the effects of such a biophysical cue on the paracrine pathway of MSCs, especially on the regulation of characteristics and bioactivity of MSC-derived exosomes have rarely been reported. In this study, we used periodontal ligament stem cells (PDLSCs), which were cultured in the three-dimensional(3D) microscale magnetically stretched collagen hydrogels, as the model MSCs to explore the changes in characteristics (e.g., morphology, size distribution, typical surface protein expression, miRNA content, and internalization) and bioactivity of MSC-derived exosomes in response to matrix strain. We found that the levels of 25 miRNAs in exosomes secreted by PDLSCs in the 3D strain microenvironment (SM-Exo) were dramatically different from those obtained from the 3D culture microenvironment (Exo). Next, the bioactivity of SM-Exo was significantly enhanced as reflected by the improved proliferation, migration, and osteogenic differentiation of target cells (e.g., BMSCs). This was further confirmed by in vivo studies, in which PDLSC-derived exosomes obtained from the 3D strain microenvironment showed stronger bioactivity to repair alveolar bone defects in SD rats. This study proposed a novel strategy to promote alveolar bone regeneration via altered PDLSC-derived exosomes in the 3D strain microenvironment, and such an engineered mechanical microenvironment would be an effective tool in other regenerative studies and clinical applications.

Keyword :

Exosomes Cell-free therapy Mesenchymal stem cells 3D cell culture Mechanical microenvironment

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Yu, Wenting , Su, Xiaoxia , Li, Moxiao et al. Three-dimensional mechanical microenvironment enhanced osteogenic activity of mesenchymal stem cells-derived exosomes [J]. | CHEMICAL ENGINEERING JOURNAL , 2021 , 417 .
MLA Yu, Wenting et al. "Three-dimensional mechanical microenvironment enhanced osteogenic activity of mesenchymal stem cells-derived exosomes" . | CHEMICAL ENGINEERING JOURNAL 417 (2021) .
APA Yu, Wenting , Su, Xiaoxia , Li, Moxiao , Wan, Wanting , Li, Ang , Zhou, Hong et al. Three-dimensional mechanical microenvironment enhanced osteogenic activity of mesenchymal stem cells-derived exosomes . | CHEMICAL ENGINEERING JOURNAL , 2021 , 417 .
Export to NoteExpress RIS BibTex
A new model of myofibroblast-cardiomyocyte interactions and their differences across species SCIE PubMed
期刊论文 | 2021 , 120 (17) , 3764-3775 | BIOPHYSICAL JOURNAL
Abstract&Keyword Cite

Abstract :

Although coupling between cardiomyocytes and myofibroblasts is well known to affect the physiology and pathophysiology of cardiac tissues across species, relating these observations to humans is challenging because the effect of this coupling varies across species and because the sources of these effects are not known. To identify the sources of cross-species variation, we built upon previous mathematical models of myofibroblast electrophysiology and developed a mechanoelectrical model of cardiomyocyte-myofibroblast interactions as mediated by electrotonic coupling and transforming growth factor-beta 1. The model, as verified by experimental data from the literature, predicted that both electrotonic coupling and transforming growth factor-beta 1 interaction between myocytes and myofibroblast prolonged action potential in rat myocytes but shortened action potential in human myocytes. This variance could be explained by differences in the transient outward K+ current associated with differential Kv4.2 gene expression across species. Results are useful for efforts to extrapolate the results of animal models to the predicted effects in humans and point to potential therapeutic targets for fibrotic cardiomyopathy.

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Liu, Fusheng , Wu, Hou , Yang, Xiaoyu et al. A new model of myofibroblast-cardiomyocyte interactions and their differences across species [J]. | BIOPHYSICAL JOURNAL , 2021 , 120 (17) : 3764-3775 .
MLA Liu, Fusheng et al. "A new model of myofibroblast-cardiomyocyte interactions and their differences across species" . | BIOPHYSICAL JOURNAL 120 . 17 (2021) : 3764-3775 .
APA Liu, Fusheng , Wu, Hou , Yang, Xiaoyu , Dong, Yuqin , Huang, Guoyou , Genin, Guy M. et al. A new model of myofibroblast-cardiomyocyte interactions and their differences across species . | BIOPHYSICAL JOURNAL , 2021 , 120 (17) , 3764-3775 .
Export to NoteExpress RIS BibTex
Matrix stiffness changes affect astrocyte phenotype in an in vitro injury model EI SCIE
期刊论文 | 2021 , 13 (1) | NPG ASIA MATERIALS
WoS CC Cited Count: 2
Abstract&Keyword Cite

Abstract :

Injury to the central nervous system (CNS) usually leads to the activation of astrocytes, followed by glial scar formation. The formation of glial scars from active astrocytes in vivo has been found to be dependent on the cell microenvironment. However, how astrocytes respond to different microenvironmental cues during scar formation, such as changes in matrix stiffness, remains elusive. In this work, we established an in vitro model to assess the responses of astrocytes to matrix stiffness changes that may be related to pathophysiology. The investigated hydrogel backbones are composed of collagen type I and alginate. The stiffness of these hybrid hydrogels can be dynamically changed by association or dissociation of alginate chains through adding crosslinkers of calcium chloride or a decrosslinker of sodium citrate, respectively. We found that astrocytes obtain different phenotypes when cultured in hydrogels of different stiffnesses. The obtained phenotypes can be switched in situ when changing matrix stiffness in the presence of cells. Specifically, matrix stiffening reverts astrogliosis, whereas matrix softening initiates astrocytic activation in 3D. Moreover, the effect of matrix stiffness on astrocytic activation is mediated by Yes-associated protein (YAP), where YAP inhibition enhances the upregulation of GFAP and contributes to astrogliosis. To investigate the underlying mechanism of matrix stiffness-dependent GFAP expression, we also developed a mathematical model to describe the time-dependent dynamics of biomolecules involved in the matrix stiffness mechanotransduction process of astrocytes. The modeling results further indicate that the effect of matrix stiffness on cell fate and behavior may be related to changes in the cytoskeleton and subsequent activity of YAP. The results from this study will guide researchers to re-examine the role of matrix stiffness in reactive astrogliosis in vivo and inspire the development of a novel therapeutic approach for controlling glial scar formation following injury, enabling axonal regrowth and improving functional recovery by exploiting the benefits of mechanobiology studies. In this work, Xu et al. established an in vitro model to assess the responses of astrocytes to the changes of matrix stiffness that may be related to pathophysiology. The investigated hydrogel backbones are composed of collagen type I and alginate. The stiffness of these hybrid hydrogels can be dynamically tuned through the association or dissociation of alginate chains, respectively. It was found that astrocytes obtain different phenotypes when cultured in hydrogels of different stiffness. The obtained phenotypes can be switched in situ when changing matrix stiffness in the presence of cells. Specifically, matrix stiffening reverts astrogliosis, whereas matrix softening initiates astrocytic activation in 3D.

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Hu, Yan , Huang, Guoyou , Tian, Jin et al. Matrix stiffness changes affect astrocyte phenotype in an in vitro injury model [J]. | NPG ASIA MATERIALS , 2021 , 13 (1) .
MLA Hu, Yan et al. "Matrix stiffness changes affect astrocyte phenotype in an in vitro injury model" . | NPG ASIA MATERIALS 13 . 1 (2021) .
APA Hu, Yan , Huang, Guoyou , Tian, Jin , Qiu, Jinbin , Jia, Yuanbo , Feng, Dayun et al. Matrix stiffness changes affect astrocyte phenotype in an in vitro injury model . | NPG ASIA MATERIALS , 2021 , 13 (1) .
Export to NoteExpress RIS BibTex
Mathematical modelling of thermocapillary patterning in thin liquid film: an equilibrium study EI SCIE
期刊论文 | 2021 , 919 | JOURNAL OF FLUID MECHANICS
Abstract&Keyword Cite

Abstract :

Thermocapillary patterning, namely, the formation of micro/nano patterns in a liquid film by surface deformation induced by an imposed thermal gradient, has enjoyed widespread applications in engineering. In this paper, we present the development of analytical and numerical models and model analyses to predict the equilibrium states of a deformed liquid polymer film under the action of thermocapillary forces. The deformation is found to be dependent on a non-dimensional parameter , with Ma denoting the Marangoni number and Ca the capillary number. Model analyses show that a hysteresis phenomenon is associated with the thermocapillary deformation of the film with increasing and then decreasing . When is increased above a critical value , significant deformation occurs in the film until the polymer touches the top solid template. Then, if is allowed to decrease, the polymer film would not detach from the template until is decreased below another critical value (usually ). With , there exist multiple (three at the maximum) equilibrium states. The Lyapunov energy analysis of these states reveals that one equilibrium state is stable, another is metastable and the third one is unstable.

Keyword :

Capillary flows Marangoni convection thin films

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Yang, Qingzhen , Li, Ben Q. , Lv, Xuemeng et al. Mathematical modelling of thermocapillary patterning in thin liquid film: an equilibrium study [J]. | JOURNAL OF FLUID MECHANICS , 2021 , 919 .
MLA Yang, Qingzhen et al. "Mathematical modelling of thermocapillary patterning in thin liquid film: an equilibrium study" . | JOURNAL OF FLUID MECHANICS 919 (2021) .
APA Yang, Qingzhen , Li, Ben Q. , Lv, Xuemeng , Song, Fenhong , Liu, Yankui , Xu, Feng . Mathematical modelling of thermocapillary patterning in thin liquid film: an equilibrium study . | JOURNAL OF FLUID MECHANICS , 2021 , 919 .
Export to NoteExpress RIS BibTex
A fast and ultrasensitive ELISA based on rolling circle amplification EI SCIE PubMed
期刊论文 | 2021 , 146 (9) , 2871-2877 | ANALYST
Abstract&Keyword Cite

Abstract :

A highly sensitive ELISA is critical for early diagnosis and biomarker discovery of various diseases. Although various ELISA technologies have been developed with high sensitivity, they are limited by poor repeatability, high cost, the dependence on complex equipment and/or a prolonged reaction time. To this end, we developed a fast and ultrasensitive ELISA (termed RELISA) based on rolling circle amplification (RCA) and enzymatic signal amplification. The RELISA is established on the traditional ELISA, with only one more RCA step that can be accomplished within 10 minutes. The prolonged single strand DNA (ssDNA) from RCA is able to enrich abundant horseradish peroxidase conjugate (HRP) modified detection probes. Consequently, the intensive HRP is able to catalyze TMB-H2O2 to produce significantly enhanced colorimetric signals. With CEACAM-7 as a model biomarker, the RELISA achieves the limit of detection as low as 2.82 pg mL(-1), which is similar to 50 times higher than that of the traditional ELISA. Therefore, we envision that the developed RELISA would be a powerful tool for the early diagnosis of various major diseases.

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 You, Minli , Peng, Ping , Xue, Zhenrui et al. A fast and ultrasensitive ELISA based on rolling circle amplification [J]. | ANALYST , 2021 , 146 (9) : 2871-2877 .
MLA You, Minli et al. "A fast and ultrasensitive ELISA based on rolling circle amplification" . | ANALYST 146 . 9 (2021) : 2871-2877 .
APA You, Minli , Peng, Ping , Xue, Zhenrui , Tong, Haoyang , He, Wanghong , Mao, Ping et al. A fast and ultrasensitive ELISA based on rolling circle amplification . | ANALYST , 2021 , 146 (9) , 2871-2877 .
Export to NoteExpress RIS BibTex
侧流试纸孔径优化数值模型 CSCD
期刊论文 | 2021 , 42 (5) , 1257-1262 | 工程热物理学报
Abstract&Keyword Cite

Abstract :

纸基微流控芯片由于成本低、比表面积大以及良好的生物兼容性等优势在即时检测领域得到快速发展,但是纸基孔隙结构对于检测性能的影响规律和机制尚不清晰.本文针对侧流试纸这一典型纸基微流控芯片,通过将硝酸纤维素膜多孔结构简化为毛细平板通道,首次建立了耦合毛细流动和反应输运过程的数值模型,研究了样品液滴流速以及硝酸纤维素膜比表面积对检测信号生成的竞争机制.研究结果表明,样品液滴流速的增加以及更大的膜比表面积均可增强检测信号,且硝酸纤维素膜孔径存在最优值以提高检测灵敏度,为硝酸纤维素膜材料的微观设计优化指明了方向.

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 贾朋朋 , 冯上升 , 徐峰 . 侧流试纸孔径优化数值模型 [J]. | 工程热物理学报 , 2021 , 42 (5) : 1257-1262 .
MLA 贾朋朋 et al. "侧流试纸孔径优化数值模型" . | 工程热物理学报 42 . 5 (2021) : 1257-1262 .
APA 贾朋朋 , 冯上升 , 徐峰 . 侧流试纸孔径优化数值模型 . | 工程热物理学报 , 2021 , 42 (5) , 1257-1262 .
Export to NoteExpress RIS BibTex
10| 20| 50 per page
< Page ,Total 43 >

Export

Results:

Selected

to

Format:
FAQ| About| Online/Total:521/105564258
Address:XI'AN JIAOTONG UNIVERSITY LIBRARY(No.28, Xianning West Road, Xi'an, Shaanxi Post Code:710049) Contact Us:029-82667865
Copyright:XI'AN JIAOTONG UNIVERSITY LIBRARY Technical Support:Beijing Aegean Software Co., Ltd.