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Anisotropic conductive reduced graphene oxide/silk matrices promote post-infarction myocardial function by restoring electrical integrity. PubMed
期刊论文 | 2021 | Acta biomaterialia
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Abstract :

Myocardial infarction (MI) remains the leading cause of death globally, often leading to impaired cardiac function and pathological myocardial microenvironment. Electrical conduction abnormalities of the infarcted myocardium not only induce adverse myocardial remodeling but also prevent tissue repair. Restoring the myocardial electrical integrity, particularly the anisotropic electrical signal propagation within the injured area after infarction is crucial for an effective function recovery. Herein, optimized reduced graphene oxide (rGO) functionalized electrospun silk fibroin (rGO/silk) biomaterials presenting anisotropic conductivity and enhanced suturablity were developed and investigated as cardiac patches for their potential in improving the post-MI myocardial function of rat models. The results show that the anisotropic conductive rGO/silk patches exhibit remarkable therapeutic effect on repairing the infarcted myocardium compared to the nonconductive silk and isotropic conductive rGO/silk patches as determined by the enhanced pumping function, reduced susceptibility to arrhythmias, thickened left ventricular walls and improved survival of functional cardiomyocytes. Their notable effect on promoting the angiogenesis of capillaries in the infarcted myocardium has also been demonstrated. This study highlights an effective and biomimetic reconstruction of the electrical myocardial microenvironment based on the anisotropic conductive rGO/silk biomaterials as a promising option for promoting the repair of infarcted myocardium. STATEMENT OF SIGNIFICANCE: The dysfunctional electrical microenvironment in the infarcted myocardium not only aggravates the adverse myocardial remodeling but also limits the effect of cardiac regenerative medicine. Although various conductive biomaterials have been employed to restore the electrical network in the infarcted myocardium in vivo, the anisotropic nature of the myocardial electrical microenvironment which enables directional electrical signal propagation were neglected. In this study, an anisotropic conductive rGO/silk biomaterial system is developed to improve the myocardial function post infarction by restoring the anisotropic electrical microenvironment in the infarcted myocardium. The promoted effects of anisotropic conductive grafts on repairing infarcted hearts are demonstrated with improved pumping function, cardiomyocyte survival, resistance to ventricular fibrillation, and angiogenesis of capillary network.

Keyword :

Electrospun scaffolds Cardiac tissue engineering Reduced graphene oxides (rGO) Conductive biomaterials Electrical microenvironment

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GB/T 7714 Zhao Guoxu , Feng Yanjing , Xue Li et al. Anisotropic conductive reduced graphene oxide/silk matrices promote post-infarction myocardial function by restoring electrical integrity. [J]. | Acta biomaterialia , 2021 .
MLA Zhao Guoxu et al. "Anisotropic conductive reduced graphene oxide/silk matrices promote post-infarction myocardial function by restoring electrical integrity." . | Acta biomaterialia (2021) .
APA Zhao Guoxu , Feng Yanjing , Xue Li , Cui Mengjie , Zhang Qi , Xu Feng et al. Anisotropic conductive reduced graphene oxide/silk matrices promote post-infarction myocardial function by restoring electrical integrity. . | Acta biomaterialia , 2021 .
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rGO/Silk Fibroin-Modified Nanofibrous Patches Prevent Ventricular Remodeling via Yap/Taz-TGF beta 1/Smads Signaling After Myocardial Infarction in Rats SCIE PubMed
期刊论文 | 2021 , 8 | FRONTIERS IN CARDIOVASCULAR MEDICINE
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Abstract :

Objective: After acute myocardial infarction (AMI), the loss of cardiomyocytes and dysregulation of extracellular matrix homeostasis results in impaired cardiac function and eventually heart failure. Cardiac patches have emerged as a potential therapeutic strategy for AMI. In this study, we fabricated and produced reduced graphene oxide (rGO)/silk fibroin-modified nanofibrous biomaterials as a cardiac patch to repair rat heart tissue after AMI and investigated the potential role of rGO/silk patch on reducing myocardial fibrosis and improving cardiac function in the infarcted rats. & nbsp; Method: rGO/silk nanofibrous biomaterial was prepared by electrospinning and vacuum filtration. A rat model of AMI was used to investigate the ability of patches with rGO/silk to repair the injured heart in vivo. Echocardiography and stress-strain analysis of the left ventricular papillary muscles was used to assess the cardiac function and mechanical property of injured hearts treated with this cardiac patch. Masson's trichrome staining and immunohistochemical staining for Col1A1 was used to observe the degree of myocardial fibrosis at 28 days after patch implantation. The potential direct mechanism of the new patch to reduce myocardial fibrosis was explored in vitro and in vivo. & nbsp; Results: Both echocardiography and histopathological staining demonstrated improved cardiac systolic function and ventricular remodeling after implantation of the rGO/silk patch. Additionally, cardiac fibrosis and myocardial stiffness of the infarcted area were improved with rGO/silk. On RNA-sequencing, the gene expression of matrix-regulated genes was altered in cardiofibroblasts treated with rGO. Western blot analysis revealed decreased expression of the Yap/Taz-TGF beta 1/Smads signaling pathway in heart tissue of the rGO/silk patch group as compared with controls. Furthermore, the rGO directly effect on Col I and Col III expression and Yap/Taz-TGF beta 1/Smads signaling was confirmed in isolated cardiofibroblasts in vitro. & nbsp; Conclusion: This study suggested that rGO/silk improved cardiac function and reduced cardiac fibrosis in heart tissue after AMI. The mechanism of the anti-fibrosis effect may involve a direct regulation of rGO on Yap/Taz-TGF beta 1/Smads signaling in cardiofibroblasts.

Keyword :

acute myocardial infarction cardiofibroblasts reduced graphene oxide myocardial fibrosis YAP/TAZ-TGF1/Smads signaling

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GB/T 7714 Feng, Yanjing , Zhao, Guoxu , Xu, Min et al. rGO/Silk Fibroin-Modified Nanofibrous Patches Prevent Ventricular Remodeling via Yap/Taz-TGF beta 1/Smads Signaling After Myocardial Infarction in Rats [J]. | FRONTIERS IN CARDIOVASCULAR MEDICINE , 2021 , 8 .
MLA Feng, Yanjing et al. "rGO/Silk Fibroin-Modified Nanofibrous Patches Prevent Ventricular Remodeling via Yap/Taz-TGF beta 1/Smads Signaling After Myocardial Infarction in Rats" . | FRONTIERS IN CARDIOVASCULAR MEDICINE 8 (2021) .
APA Feng, Yanjing , Zhao, Guoxu , Xu, Min , Xing, Xin , Yang, Lijun , Ma, Yao et al. rGO/Silk Fibroin-Modified Nanofibrous Patches Prevent Ventricular Remodeling via Yap/Taz-TGF beta 1/Smads Signaling After Myocardial Infarction in Rats . | FRONTIERS IN CARDIOVASCULAR MEDICINE , 2021 , 8 .
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Bio-inspired Janus structural color films as visually flexible electronics EI SCIE
期刊论文 | 2021 , 24 | APPLIED MATERIALS TODAY
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Multifunctional composite flexible sensors are essential components in flexible and stretchable electronics. In this paper, we present novel mussel-inspired Janus structural color films as visually flexible electronics. The Janus composite film with a three-layer structure, which is compozed of a conductive carbon nanotube (CNT) layer, a supporting polydimethylsiloxane (PDMS) layer and a structural color layer formed by two-dimensional colloidal crystals (2D-CCs), is prepared by a hierarchical assembly strategy. The manufactured multi-layer CNT films and the 2D-CCs are integrated on both sides of the supporting PDMS layer, respectively by the self-assembly process and the adhesion of polydopamine (PDA) to provide corresponding excellent conductivity, flexibility and visual optical sensing for the Janus composite film. It is demonstrated that the CNT films could not only increaze the contrast of structural color of the 2D-CC array as their light absorption characteristics in the broadband frequency field, but also impart the composite film with photothermal response characteristics. In addition, because of the outstanding electrical properties, visualized structural color and photothermal response, the resulting Janus structural color films show stable electrical sensing and visualized color-sensing under deformations arose by human motions and near-infrared (NIR) illumination, which could play essential roles in flexible and stretchable electronics. (c) 2021 Elsevier Ltd. All rights reserved.

Keyword :

Flexible electronics Photothermal response Bioinspired Carbon nanotube Structural color

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GB/T 7714 Xu, Dongyu , Sun, Lingyu , Zhang, Zhuohao et al. Bio-inspired Janus structural color films as visually flexible electronics [J]. | APPLIED MATERIALS TODAY , 2021 , 24 .
MLA Xu, Dongyu et al. "Bio-inspired Janus structural color films as visually flexible electronics" . | APPLIED MATERIALS TODAY 24 (2021) .
APA Xu, Dongyu , Sun, Lingyu , Zhang, Zhuohao , Wang, Yu , Zhang, Xiaohui , Ye, Fangfu et al. Bio-inspired Janus structural color films as visually flexible electronics . | APPLIED MATERIALS TODAY , 2021 , 24 .
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A wearable, nozzle-diffuser microfluidic pump based on high-performance ferroelectric nanocomposites EI SCIE
期刊论文 | 2021 , 347 | SENSORS AND ACTUATORS B-CHEMICAL
WoS CC Cited Count: 1
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Abstract :

New target applications for microfluidic devices have been focused on home usability, wearability and cost-effectiveness. Towards these goals, in this work, a controllable, bendable, and all-organic, nozzle-diffuser microfluidic pump was designed, fabricated and tested. First, to resolve the crucial issue of high driving voltage, the ferroelectric polymer poly(vinylidene fluoride-trifluoroethylene) (P(VDF-TrFE)) was optimized by adding core-shell structured Al2O3@CNT nanofillers. The membrane so developed with 1.1 wt% Al2O3@CNT showed an increase by nearly 7 times in the induced strain in comparison to the neat P(VDF-TrFE) at low electric fields, because the modified membrane simultaneously achieved a lower coercive electric field and a higher polarization. Accordingly, the required operating voltage of the microfluidic pump integrated with optimized membrane significantly decreased from 1000 V to 160 V. Meanwhile, this pump exhibited a wider range of flow rates (13-135 mu L/min) than the reported results, associated with the higher output pressure in our membranes. Importantly, the designed pump still possessed an excellent controllability of the fluidic processes, though it underwent a large bending up to 74 degrees. Consequently, the extremely promising application of the as-prepared microfluidic pump in wearable, biomedical devices was demonstrated.

Keyword :

Microfluidic pump Electromechanical performance Ferroelectric nanocomposites Core-shell structure Interfacial polarization

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GB/T 7714 Zhao, Fengwan , Chen, Xiaoming , Zhang, Jie et al. A wearable, nozzle-diffuser microfluidic pump based on high-performance ferroelectric nanocomposites [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2021 , 347 .
MLA Zhao, Fengwan et al. "A wearable, nozzle-diffuser microfluidic pump based on high-performance ferroelectric nanocomposites" . | SENSORS AND ACTUATORS B-CHEMICAL 347 (2021) .
APA Zhao, Fengwan , Chen, Xiaoming , Zhang, Jie , Zhang, Xiaohui , Xie, Jun , Jin, Li et al. A wearable, nozzle-diffuser microfluidic pump based on high-performance ferroelectric nanocomposites . | SENSORS AND ACTUATORS B-CHEMICAL , 2021 , 347 .
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The Plasticity of Nanofibrous Matrix Regulates Fibroblast Activation in Fibrosis EI SCIE PubMed
期刊论文 | 2021 , 10 (8) | ADVANCED HEALTHCARE MATERIALS
WoS CC Cited Count: 2
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Abstract :

Natural extracellular matrix (ECM) mostly has a fibrous structure that supports and mechanically interacts with local residing cells to guide their behaviors. The effect of ECM elasticity on cell behaviors has been extensively investigated, while less attention has been paid to the effect of matrix fiber-network plasticity at microscale, although plastic remodeling of fibrous matrix is a common phenomenon in fibrosis. Here, a significant decrease is found in plasticity of native fibrotic tissues, which is associated with an increase in matrix crosslinking. To explore the role of plasticity in fibrosis development, a set of 3D collagen nanofibrous matrix with constant modulus but tunable plasticity is constructed by adjusting the crosslinking degree. Using plasticity-controlled 3D culture models, it is demonstrated that the decrease of matrix plasticity promotes fibroblast activation and spreading. Further, a coarse-grained molecular dynamic model is developed to simulate the cell-matrix interaction at microscale. Combining with molecular experiments, it is revealed that the enhanced fibroblast activation is mediated through cytoskeletal tension and nuclear translocation of Yes-associated protein. Taken together, the results clarify the effects of crosslinking-induced plasticity changes of nanofibrous matrix on the development of fibrotic diseases and highlight plasticity as an important mechanical cue in understanding cell-matrix interactions.

Keyword :

nanofibrous matrix fiber networks cell traction fibrosis mechanical plasticity

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GB/T 7714 Jia, Yuanbo , Wang, Yanzhong , Niu, Lele et al. The Plasticity of Nanofibrous Matrix Regulates Fibroblast Activation in Fibrosis [J]. | ADVANCED HEALTHCARE MATERIALS , 2021 , 10 (8) .
MLA Jia, Yuanbo et al. "The Plasticity of Nanofibrous Matrix Regulates Fibroblast Activation in Fibrosis" . | ADVANCED HEALTHCARE MATERIALS 10 . 8 (2021) .
APA Jia, Yuanbo , Wang, Yanzhong , Niu, Lele , Zhang, Hang , Tian, Jin , Gao, Dengfeng et al. The Plasticity of Nanofibrous Matrix Regulates Fibroblast Activation in Fibrosis . | ADVANCED HEALTHCARE MATERIALS , 2021 , 10 (8) .
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Microfluidic Printing of Three-Dimensional Graphene Electroactive Microfibrous Scaffolds EI SCIE Scopus
期刊论文 | 2020 , 12 (2) , 2049-2058 | ACS APPLIED MATERIALS & INTERFACES | IF: 9.229
WoS CC Cited Count: 18 SCOPUS Cited Count: 19
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Abstract :

Graphene materials have attracted special attention because of their electrical conductivity, mechanical properties, and favorable biocompatibility. Although various methods have been developed for fabricating micro/nano conductive fibrous scaffolds, it is still challenging to fabricate the three-dimensional (3D) graphene fibrous scaffolds. Herein, we developed a new method, termed as microfluidic 3D printing technology (M3DP), to fabricate 3D graphene oxide (GO) microfibrous scaffolds with an adjustable fiber length, fiber diameter, and scaffold structure by integrating the microfluidic spinning technology with a programmable 3D printing system. GO microfibrous scaffolds were then transformed into conductive reduced graphene oxide (rGO) microfibrous scaffolds by hydrothermal reduction. Our results demonstrated that the fabricated 3D fibrous graphene scaffolds exhibited tunable structures, maneuverable mechanical properties, and good electrical conductivity and biocompatibility, as reflected by the adhesion and proliferation of SH-SY5Y cells on the graphene microfibrous scaffolds in an obviously oriented manner. The developed M3DP would be a powerful tool for fabricating 3D graphene microfibrous scaffolds for electroactive tissue regeneration and drug-screening applications.

Keyword :

tissue engineering graphene fiber graphene oxide 3D printing microfluidic spinning

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GB/T 7714 Qing, Huaibin , Ji, Yuan , Li, Wenfang et al. Microfluidic Printing of Three-Dimensional Graphene Electroactive Microfibrous Scaffolds [J]. | ACS APPLIED MATERIALS & INTERFACES , 2020 , 12 (2) : 2049-2058 .
MLA Qing, Huaibin et al. "Microfluidic Printing of Three-Dimensional Graphene Electroactive Microfibrous Scaffolds" . | ACS APPLIED MATERIALS & INTERFACES 12 . 2 (2020) : 2049-2058 .
APA Qing, Huaibin , Ji, Yuan , Li, Wenfang , Zhao, Guoxu , Yang, Qingzhen , Zhang, Xiaohui et al. Microfluidic Printing of Three-Dimensional Graphene Electroactive Microfibrous Scaffolds . | ACS APPLIED MATERIALS & INTERFACES , 2020 , 12 (2) , 2049-2058 .
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Matrix stiffness controls cardiac fibroblast activation through regulating YAP via AT1 R. PubMed SCIE
期刊论文 | 2020 , 235 (11) , 8345-8357 | Journal of cellular physiology | IF: 6.384
WoS CC Cited Count: 9
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Abstract :

Cardiac fibrosis is a common pathway leading to heart failure and involves continued activation of cardiac fibroblasts (CFs) into myofibroblasts during myocardium damage, causing excessive deposition of the extracellular matrix (ECM) and thus increases matrix stiffness. Increasing evidence has shown that stiffened matrix plays an important role in promoting CF activation and cardiac fibrosis, and several signaling factors mediating CF mechanotransduction have been identified. However, the key molecules that perceive matrix stiffness to regulate CF activation remain to be further explored. Here, we detected significantly increased expression and nuclear localization of Yes-associated protein (YAP) in native fibrotic cardiac tissues. By using mechanically regulated in vitro cell culture models, we found that a stiff matrix-induced high expression and nuclear localization of YAP in CFs, accompanied by enhanced cell activation. We also demonstrated that YAP knockdown decreased fibrogenic response of CFs and that YAP overexpression promoted CF activation, indicating that YAP plays an important role in mediating matrix stiffness-induced CF activation. Further mechanistic studies revealed that the YAP pathway is an important signaling branch downstream of angiotensin II type 1 receptor in CF mechanotransduction. The findings help elucidate the mechanism of fibrotic mechanotransduction and may contribute to the development of new approaches for treating fibrotic diseases.

Keyword :

Yes-associated protein mechanotransduction hydrogel mechanical microenvironment cardiac fibrosis

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GB/T 7714 Niu Lele , Jia Yuanbo , Wu Mian et al. Matrix stiffness controls cardiac fibroblast activation through regulating YAP via AT1 R. [J]. | Journal of cellular physiology , 2020 , 235 (11) : 8345-8357 .
MLA Niu Lele et al. "Matrix stiffness controls cardiac fibroblast activation through regulating YAP via AT1 R." . | Journal of cellular physiology 235 . 11 (2020) : 8345-8357 .
APA Niu Lele , Jia Yuanbo , Wu Mian , Liu Han , Feng Yanjing , Hu Yan et al. Matrix stiffness controls cardiac fibroblast activation through regulating YAP via AT1 R. . | Journal of cellular physiology , 2020 , 235 (11) , 8345-8357 .
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Solvent-Free Fabrication of Carbon Nanotube/Silk Fibroin Electrospun Matrices for Enhancing Cardiomyocyte Functionalities EI SCIE PubMed
期刊论文 | 2020 , 6 (3) , 1630-1640 | ACS Biomaterials Science and Engineering
WoS CC Cited Count: 23
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Abstract :

Cardiac tissue engineering holds great potential in regenerating functional cardiac tissues for various applications. The major strategy is to design scaffolds recapitulating the native cardiac microenvironment to enhance cell and tissue functionalities. Among various biomaterial systems, nanofibrous matrices with aligned morphologies and enhanced conductivity incline to induce the formation of oriented engineered cardiac tissues with enhanced functionalities. The challenge is to functionalize the scaffolds with conductive additives without influencing their biocompatibility. In this study, we developed a fully aqueous process for the fabrication of conductive carbon nanotube/silk fibroin (CNT/silk) electrospun scaffolds. The carbon nanotubes are well dispersed within the nanofibers, providing the scaffolds with enhanced conductivity and excellent biocompatibility for the culture of neonatal rat cardiomyocytes with improved cell spreading and enhanced expression of cardiac-specific proteins. Moreover, the aligned CNT/silk fibroin composite scaffolds exhibit abilities to guide the oriented organization of cardiac tissues and the biomimicking distribution of sarcomeres and gap junctions. The findings demonstrate the great potential of the CNT/silk scaffolds prepared through this aqueous processing method in supporting the formation of cardiac tissues with enhanced functionalities. Copyright © 2020 American Chemical Society.

Keyword :

Scaffolds (biology) Additives Nanofibers Biocompatibility Fabrication Heart Histology Carbon nanotubes Electrospinning Tissue Tissue regeneration

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GB/T 7714 Zhao, Guoxu , Zhang, Xu , Li, Bingcheng et al. Solvent-Free Fabrication of Carbon Nanotube/Silk Fibroin Electrospun Matrices for Enhancing Cardiomyocyte Functionalities [J]. | ACS Biomaterials Science and Engineering , 2020 , 6 (3) : 1630-1640 .
MLA Zhao, Guoxu et al. "Solvent-Free Fabrication of Carbon Nanotube/Silk Fibroin Electrospun Matrices for Enhancing Cardiomyocyte Functionalities" . | ACS Biomaterials Science and Engineering 6 . 3 (2020) : 1630-1640 .
APA Zhao, Guoxu , Zhang, Xu , Li, Bingcheng , Huang, Guoyou , Xu, Feng , Zhang, Xiaohui . Solvent-Free Fabrication of Carbon Nanotube/Silk Fibroin Electrospun Matrices for Enhancing Cardiomyocyte Functionalities . | ACS Biomaterials Science and Engineering , 2020 , 6 (3) , 1630-1640 .
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Flexible CoFeB/Silk Films for Biocompatible RF/Microwave Applications. EI PubMed SCIE Scopus
期刊论文 | 2020 , 12 (46) , 51654-51661 | ACS applied materials & interfaces | IF: 9.229
WoS CC Cited Count: 2 SCOPUS Cited Count: 3
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Abstract :

Aiming to realize the integration of signal processing with the human body, wearable and implantable radio frequency (RF)/microwave devices are rapidly developed. However, the insufficiency in skin commonality and biocompatibility brings up challenges in making such devices. In this study, an ultra-flexible and biocompatible CoFeB/silk film is developed potentially for on-skin and implantable RF/microwave applications. Moreover, the CoFeB/silk films present controllable dissolvability in aqueous solutions and exhibit great potentials in applications for environmentally friendly disposable devices. A strain-tunable bandstop filter based on the CoFeB/silk film was fabricated, which exhibited a large frequency tunability of 3 GHz and ability in monitoring the finger-joints' motions. This concept and method of combining ferromagnetic materials with the biocompatible substrate offers a novel pathway for wearable/implantable applications.

Keyword :

silk fibroin magnetostriction magnetic thin films RF/microwave ferromagnetic resonance

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GB/T 7714 Zhang Qi , Peng Bin , Zhao Yanan et al. Flexible CoFeB/Silk Films for Biocompatible RF/Microwave Applications. [J]. | ACS applied materials & interfaces , 2020 , 12 (46) : 51654-51661 .
MLA Zhang Qi et al. "Flexible CoFeB/Silk Films for Biocompatible RF/Microwave Applications." . | ACS applied materials & interfaces 12 . 46 (2020) : 51654-51661 .
APA Zhang Qi , Peng Bin , Zhao Yanan , Li Chunlei , Zhu Shukai , Shi Keqing et al. Flexible CoFeB/Silk Films for Biocompatible RF/Microwave Applications. . | ACS applied materials & interfaces , 2020 , 12 (46) , 51654-51661 .
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最小体积玻璃化冻存技术在生物医学领域的进展和应用 CQVIP CSCD
期刊论文 | 2019 , 40 (1) , 41-58 | 中国科学:生命科学
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Abstract :

细胞和组织的生物保存在辅助生殖医学、 再生医学和器官移植等领域均具有至关重要的作用, 对公共健康具有广泛影响. 生物保存方法主要包括传统的程序化慢冻法(slow freezing)和玻璃化冻存技术(vitrification). 玻璃化冻存技术可通过降低或避免结晶的产生而显著提高细胞解冻后的活性和功能. 但玻璃化冻存技术通常采用较高浓度的冷冻保护剂(cryoprotectant agent, CPA), 会对细胞和组织产生一定的毒性和渗透压力. 研究显示, 通过减小玻璃化冻存中样品的体积(<1 µL)可显著提高冷却速率, 从而降低玻璃化冻存所需的CPA浓度. 而微纳技术的迅速发展使得皮升至纳升量级样本的操作成为可能. 因此, 微纳技术与低温学的有机结合将为未来生物保存技术的发展提供巨大的潜能.

Keyword :

冷冻保存 冷冻保护剂 微液滴玻璃化冻存 最小体积玻璃化冻存

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GB/T 7714 张淇 , 赵国旭 , 刘艺 et al. 最小体积玻璃化冻存技术在生物医学领域的进展和应用 [J]. | 中国科学:生命科学 , 2019 , 40 (1) : 41-58 .
MLA 张淇 et al. "最小体积玻璃化冻存技术在生物医学领域的进展和应用" . | 中国科学:生命科学 40 . 1 (2019) : 41-58 .
APA 张淇 , 赵国旭 , 刘艺 , 张晓慧 . 最小体积玻璃化冻存技术在生物医学领域的进展和应用 . | 中国科学:生命科学 , 2019 , 40 (1) , 41-58 .
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