Optical　coherence　tomography　(OCT)　is　one　of　the　rapidly　developed　techniques　for　bio-tomography　in　the　last　few　decades.　It　can　acquire　the　high　resolution　cross-section　image　of　micro　mechanism　in　the　interior　organism　tissue.　Due　to　its　advantages,　including　rapid　imaging,　non-invasive　detecting　and　high　resolution,　it　has　been　widely　applied　in　biomedical　fields,　especially　in　early　diagnosis　and　dynamic　monitoring　for　the　systems　of　eyes,　skin,　oral　cavity　and　cardiovascular.　The　Michelson　interferometer　for　optical　coherence　tomography　technology　is　uesed,　an　OCT　system　is　designed　and　combined.　The　physical　level　concentrations　of　glucose　solutions　are　measured.　The　relationship　between　the　distance　of　the　interference　wave　images　on　the　highest　and　the　lowest　and　the　sample　concentration　is　explored.　In　the　laboratory,　the　samples　on　physiological　level　are　obtained　from　the　compound　5%　glucose　solution.　The　results　show　that　the　linear　coefficient　is　0.999　8,　and　the　standard　deviation　is　0.003　9.　Using　OCT　system　by　the　related　features　between　sample　concentration　and　the　distance　of　interference　image　wave　from　the　highest　to　the　lowest　can　measure　the　concentration　of　glucose　solution　accurately　and　rapidly.　This　new　method　can　be　used　for　the　study　of　non-invasive　blood　glucose　measurement　instrument.