©　2016,　Editorial　Board　of　Journal　of　Xi＇an　Jiaotong　University　(Medical　Sciences).　All　right　reserved.　Objective:　To　investigate　the　effects　of　phenytoin　(PHT)　on　the　secretion　of　vascular　endothelial　growth　factor　(VEGF)　and　stem　cell　factor　(SCF)　based　on　the　establishment　of　indirect　co-culture　system　of　rat　bone　marrow　mesenchymal　stem　cells　(BMSCs)　and　vascular　endothelial　cells　(VECs).　Methods:　Indirect　co-culture　model　of　rat　BMSCs　and　VECs　was　established.　Experimental　groups:　indirect　co-culture　groups　(PHT　concentrations　were　0,　20　and　40　μg/mL);　the　control　group:　BMSCs　culture　group　and　VECs　culture　group　(PHT　concentrations　were　0,　20　and　40　μg/mL).　The　contents　of　VEGF　and　SCF　in　the　culture　supernatant　were　measured　using　double　antibody　sandwich　ABC-ELISA　method　on　cultivation　days　2,　4,　6.　Results:　ELISA　assay　of　the　rBMSCs　and　rVECs　in　indirect　co-culture　supernatants,　collected　on　culture　days　2,　4　and　6　showed　that:　①　VEGF:　On　culture　day　2,　VEGF　level　in　the　co-culture　groups　was　significantly　higher　than　those　in　BMSCs　group　(P＜0.05)　and　rVECs　group　(P＜0.001).　As　culture　time　prolonged　and　PHT　concentration　increased　to　20　μg/mL　and　40　μg/mL,　VEGF　level　increased　too　(P＜0.001,　P＜0.05).　②　SCF　testing　results　showed　that　the　secretion　of　SCF　in　co-culture　groups　was　higher　than　that　in　the　control　groups.　When　PHT　was　20　μg/mL,　the　secretion　of　SCF　increased　as　the　incubation　time　increased;　but　as　the　incubation　time　increased,　PHT　concentration　of　40　μg/mL　made　SCF　content　decrease.　Each　group　did　not　significantly　differ　(P＞0.05).　Conclusion:　PHT　promotes　the　secretion　of　VEGF　and　may　reduce　the　secretion　of　SCF.