12　Cancer　tissue　samples,　14　inflammatory　samples　and　10　normal　samples　of　gallbladder　and　their　corresponding　tissue　membranes　and　gallbladder　cancer　cell　lines　were　observed　via　FTIR.　Compared　to　normal　gallbladder　tissue,　the　intensities　of　bands,　related　to　lipids,　at　2955,　2920,　2870,　2850　and　1740,　decreased　for　gallbladder　cancer　tissue,　the　intensity　of　bands,　related　to　protein,　at　1550　cm(-1)　decreased　for　gallbladder　cancer　tissue;　the　intensities　of　lipids　bands　and　protein　bands　increased　for　gallbladder　cancer　cell　line　obviously;　the　intensities　of　lipids　related　bands　also　increased　for　gallbladder　cancer　tissue　membrane;　the　intensity　of　band　related　to　nucleic　acid　for　gallbladder　cancer　tissue　is　similar　to　that　for　gallbladder　cancer　cell　line　and　the　band　disappeared　for　gallbladder　cancer　tissue　plasma　membrane;　the　ratio　of　I-1460/I-1400　changed　uniformity　for　tissue,　cell　line　and　tissue　plasma　membrane,　which　is　smaller　for　cancer　ones　and　bigger　for　normal　ones.　These　results　indicate　that　compared　with　those　of　gallbladder　cancer　tissue,　the　FTIR　spectra　changed　a　lot　for　gallbladder　cancer　cell　lines　and　gallbladder　cancer　tissue　plasma　membrane.　Protein　and　lipids　relatively　decreased　in　gallbladder　cancer　tissues　but　increased　in　gallbladder　cancer　cell　line　and　gallbladder　cancer　tissue　plasma　membrane.　The　paper　expounds　FTIR　variation　for　carcinogenesis　from　tissue,　cell　line　and　tissue　plasma　membrane.　The　ratio　of　I-1460/I-1400　changed　uniformity　for　tissue,　cell　line　and　tissue　plasma　membrane,　more　studies　may　get　a　fine　clinical　diagnosis　index.