Previous　studies　have　confirmed　that　motor　neuron　apoptosis　in　the　anterior　horn　of　the　lumbosacral　spinal　cord　is　positively　correlated　with　p75　neurotrophin　receptor　(p75NTR)　expression　in　rat　models　of　cauda　equina　syndrome.　This　study　used　adenovirus　to　carry　a　short　hairpin　RNA　(shRNA)　for　p75NTR　gene　silencing,　to　reduce　p75NTR　expression　in　the　damaged　phase　and　to　decrease　motor　neuron　apoptosis.　Three　p75　siRNA　template　oligonucleotide　segments　(shRNA)　were　designed,　and　cloned　into　the　1.0　CMV　shuttle　vector.　HEK293　cells　were　cotransfected　with　shuttle　vector　(carrying　shRNA)　and　an　adenovirus　vector　framework　expressing　enhanced　green　fluorescent　protein.　Thus,　this　study　successfully　obtained　adenovirus　carrying　p75shRNA.　The　obtained　viruses　were　named　Ad.shRNA1,　Ad.shRNA2,　and　Ad.shRNA3.　The　recombinant　adenoviruses　were　separately　used　to　infect　cultured　pheochromocytoma　cells　(PC12).　Forty-eight　hours　later,　p75NTR　mRNA　and　total　protein　were　analyzed　from　the　PC12　cells.　Compared　with　the　negative　controls,　RNA　interference　rates　were　separately　98.49　+/-　0.68%,　95.08　+/-　1.79%　and　96.60　+/-　1.14%　at　the　mRNA　level,　and　72.89　+/-　2.17%,　58.83　+/-　1.15%　and　59.88　+/-　0.44%　at　the　protein　level　in　the　Ad.shRNA1,　Ad.shRNA2,　and　Ad.shRNA3　groups,　respectively.　Thus,　recombinant　adenovirus　shRNA-mediated　gene　silencing　successfully　suppressed　p75NTR　expression.