This　study　explored　the　double　lethal　effects　of　pEGFP-C1-wtp53/junB　fusion　gene　on　hepatocellular　carcinoma　(HCC)　cells.　wtp53/junB　fusion　gene　was　constructed　and　transformed　into　HepG2　cell　line.　Expression　of　KAI1　was　detected　by　quantitative　real-time　PCR　and　Western　blotting,　cells　apoptosis　rate　was　detected　by　flow　cytometry,　proliferation　of　cells　was　detected　by　MTT　chromometry,　cell　transmigration　was　detected　by　using　transwell　systems.　The　results　showed　that　after　transformation　with　pEGFP-C1-wtp53/JunB,　the　expression　level　of　KAI1　protein　was　up-regulated,　being　8.13　times　the　blank　control　group　in　HepG2　cells　and　significantly　higher　than　2.87　times　which　transformed　with　pEGFP-C1-JunB,　3.11　times　which　transformed　with　pEGFP-C1-wtp53　(P　＜　0.001).　Apoptosis　rate　of　HepG2　cells　transformed　with　pEGFP-C1-wtp53/JunB　was　significantly　higher　than　that　of　other　groups　(P　＜　0.001),　and　invasive　ability　of　HepG2　cells　transformed　with　pEGFP-C1-wtp53/JunB　was　significantly　lower　than　other　groups　(P　＜　0.001).　It　was　concluded　that　the　fusion　gene　of　wtp53　and　JunB　could　not　only　inhibit　the　growth　of　hepatoma　cells　and　promote　tumor　cell　apoptosis,　but　also　suppress　the　invasive　ability　of　tumor　cells　by　up-regulating　the　expression　of　KAI1.