In　this　study,　polylactic　acid　(PLA)-based　composite　scaffolds　with　calcium　carbonate　(CaCO3)　and　beta-tricalcium　phosphate　(beta-TCP)　were　obtained　by　3D　printing.　These　structures　were　evaluated　as　potential　3D　structures　for　bone　tissue　regeneration.　Morphological,　mechanical,　and　biological　tests　were　carried　out　in　order　to　compare　the　effect　of　each　additive　(added　in　a　concentration　of　5%　w/w)　and　the　combination　of　both　(2.5%　w/w　of　each　one),　on　the　PLA　matrix.　The　scaffolds　manufactured　had　a　mean　pore　size　between　400-425　mu　m　and　a　porosity　value　in　the　range　of　50-60%.　According　to　the　results,　both　additives　promoted　an　increase　of　the　porosity,　hydrophilicity,　and　surface　roughness　of　the　scaffolds,　leading　to　a　significant　improvement　of　the　metabolic　activity　of　human　osteoblastic　osteosarcoma　cells.　The　best　results　in　terms　of　cell　attachment　after　7　days　were　obtained　for　the　samples　containing　CaCO3　and　beta-TCP　particles　due　to　the　synergistic　effect　of　both　additives,　which　results　in　an　increase　in　osteoconductivity　and　in　a　microporosity　that　favours　cell　adhesion.　These　scaffolds　(PLA:CaCO3:beta-TCP　95:2.5:2.5)　have　suitable　properties　to　be　further　evaluated　for　bone　tissue　engineering　applications.