Background　Laryngeal　squamous　cell　carcinoma　(LSCC)　is　one　of　the　most　prevalent　malignant　neoplasms　of　the　upper　respiratory　tract.　Studies　have　confirmed　that　an　unstable　chromosome　constitution　promotes　the　progress　of　laryngeal　tumorigenesis,　and　ACYP2　has　been　confirmed　as　a　telomere　length-related　gene.　However,　to　date,　the　association　between　ACYP2　polymorphisms　and　LSCC　susceptibility　has　not　been　investigated.　Methods　We　performed　this　study　to　explore　the　effect　of　11　single-nucleotide　polymorphisms　(SNPs)　in　ACYP2　on　LSCC　susceptibility　in　Chinese　Han　males.　Unconditional　logistic　regression　analysis　adjusted　for　age　was　used　to　calculate　the　odds　ratios　and　95%　confidence　intervals.　Results　Based　on　allele　and　genotype　models,　our　results　showed　that　rs1682111　variant　was　significantly　associated　with　a　decreased　LSCC　susceptibility　(p　＜　0.05).　On　the　contrary,　polymorphisms　of　rs10439478,　rs11125529,　rs12615793,　rs843711,　rs11896604,　and　rs17045754　were　significantly　associated　with　an　increased　LSCC　risk　(p　＜　0.05).　The　results　of　haplotype　analysis　indicated　that　haplotypes　＂TTCTCG＂　and　＂TTCTAA＂　in　block　1　and　＂TG＂　in　block　2　showed　a　risk　factor　for　the　development　of　LCSS　(p　=　0.009,　p　＜　0.001,　and　p　=　0.001,　respectively).　The　results　of　Genotype-Tissue　Expression　analysis　indicate　that　these　significant　SNPs　were　known　to　be　associated　with　ACYP2　expression.　Conclusion　Our　data　demonstrated　that　ACYP2　polymorphisms　may　exert　effects　on　LSCC　susceptibility　in　Chinese　Han　males.