Background　Mechanical　stimuli　can　cause　periodontal　tissue　reconstruction.　Studies　have　found　that　changes　in　metabolites　can　be　the　terminal　effect　of　integrin-mediated　mechanical　signaling.　As　a　key　kinase　in　integrin　regulation,　integrin-linked　kinase　(ILK)　mediates　mechanical　signal　transduction,　which　may　contribute　to　metabolite　changes.　Defining　the　components　of　small-molecule　metabolites　can　optimize　mechanical　stimuli　and　periodontal　tissue　reconstruction.　Our　purpose　is　to　detect　the　effect　of　ILK-mediated　mechanical　signaling　on　intracellular　small-molecule　metabolites　(amino　acids　and　organic　acids)　in　human　periodontal　ligament　fibroblasts　(HPDLFs).　Methods　Primary　HPDLFs　were　isolated　by　enzyme　digestion　method.　Tensile　stresses　were　applied　on　HPDLFs　in　vitro　using　a　Flexcell　system.　ILK　gene　in　HPDLFs　was　knocked　down　by　RNA　interference　(RNAi).　Twenty　common　amino　acids　and　seven　organic　acids　in　HPDLFs　were　analyzed　by　gas　chromatography/mass　spectrometry　technique.　Results　Five　amino　acids　(ie,　alanine,　glutamine,　glutamate,　glycine,　and　threonine)　and　three　organic　acids　(ie,　pyruvate,　lactate,　and　citric　acid)　were　found　to　be　changed　remarkably　after　mechanical　stretching.　In　addition,　baseline　levels　of　four　amino　acids　(ie,　glutamate,　glutamine,　threonine,　and　glycine)　and　two　organic　acids　(ie,　lactate　and　citric　acid)　were　significantly　different　in　ILK　knockdown　compared　with　wild-type　HPDLFs.　Conclusion　This　study　suggests　that　five　amino　acids　(ie,　alanine,　glutamine,　glutamate,　glycine,　and　threonine)　and　three　organic　acids　(ie,　pyruvate,　lactate,　and　citric　acid)　may　act　as　cellular　mediators　for　mechanical　signals　in　HPDLFs.　Among　them,　four　amino　acids　(ie,　glutamate,　glutamine,　threonine,　and　glycine)　and　two　organic　acids　(ie,　lactate　and　citric　acid)　may　be　closely　linked　to　ILK.