Natural　biomaterials　are　extensively　used　in　tissue　engineering　due　to　their　microstructure　interconnectivity　and　inherent　bioactivity　which　mimics　of　natural　extracellular　matrix　(ECM),　supporting　cell　infiltration,　adhesion,　differentiation,　transportation　of　oxygen　and　nutrient,　finally　restoring　the　structure　and　function　of　defective　tissues　or　organs.　Microstructure,　mechanical　properties,　biostability　and　cellular　activity　of　natural　biomaterials　are　controlled　via　blending　of　natural　or　natural　with　synthetic　biopolymers　and　physical/chemical　crosslinking　treatments　to　allow　the　required　mechanical　strength,　degradation　rate　and　ECM　mimic　microenvironment　for　supporting　of　cellular　activity.　In　addition,　natural　biomaterials　also　performed　a　key　role　in　delivery　of　cells,　bioactive　molecules,　growth　factors　and　drugs.　In　this　review,　we　will　explore　the　fabrication,　challenges　and　applications　of　natural　biomaterials　for　various　tissues　engineering　issues,　including　polymer　selection,　fabrication　techniques,　microstructure　manipulation,　physical/chemical　crosslinking,　mechanical　properties,　biostability　as　well　as　their　role　in　delivery　of　cells,　bioactive　molecules,　growth　factors　and　drugs.　©　2020　Elsevier　Ltd