In　this　paper,　we　look　into　the　correction　on　the　ambiguities　in　the　conversion　between　genome-based　coordinates　and　RNA-based　coordinates.　An　improved　algorithm　for　estimating　the　distribution　of　RNA-related　genomic　features　is　proposed　based　on　our　previous　article,　＇Guitar:　An　R/Bioconductor　Package　for　Gene　Annotation　Guided　Transcriptomic　Analysis　of　RNA-Related　Genomic　Features＇.　It　applies　Expectation　Maximization　algorithm　to　estimate　RNA-related　genomic　features　using　iterations.　After　each　iteration,　the　proportion　of　the　real　distribution　coordinates　is　increased,　and　the　result　is　closer　to　the　real　distribution,　demonstrating　validity　and　effectiveness　of　our　proposed　method.　©　2020　ACM.