A　method　for　rapid　analysis　of　ractopamine　in　porcine　muscle　and　urine　was　developed　and　validated.　The　method　was　based　on　combined　of　microextraction　by　packed　sorbent　(MEPS)　and　high-performance　liquid　chromatography　with　ultraviolet　detection　(HPLC-UV).　Parameters　of　the　MEPS　procedure　affecting　extraction　efficiency　were　optimised.　Optimum　extraction　conditions　were　100　mu　L　of　sample　in　five　extraction　cycles　and　sampling　time　of　＜3　min.　Compared　with　solid-phase　extraction,　the　MEPS　procedure　required　less　extraction　time,　sample　volume　and　consumption　of　organic　solvents.　The　method　demonstrated　high　linearity　within　0.01-2　mu　g/mL　for　porcine　muscle　and　urine　samples　(R-2　＞　0.9985).　Accuracies　of　muscle　and　urine　analyses　were　93.9-109.2%　and　93.4-105.1%,　respectively.　Intra-day　and　inter-day　precisions　(RSD%)　were　lower　than　11.8%　for　both　analyses.　The　method　was　applied　for　rapid　analysis　of　ractopamine　in　biological　samples.　The　method　was　simpler　and　could　be　used　to　screen　other　beta(2)-agonists　in　other　extraction　media.　(C)　2013　Elsevier　Ltd.　All　rights　reserved.