Extracellular　supplement　of　Ca　and　Si　accelerates　osteogenisis　and　angiogenisis　during　bone　repair.　Ca-Si-based　ceramics　are　biodegradable　and　can　release　Ca　and　Si　ions　efficiently.　However,　locally　high　alkalinity　induced　by　excessively　rapid　degradation　limits　their　application.　In　this　paper,　NaCa2HSi3O9　(NCS)　nanorods　were　fabricated　on　Ti　by　micro-arc　oxidation　and　hydrothermal　treatment;　protein　layers　consisted　of　different　rations　of　bovine　serum　albumin　(BSA)　and　lysozyme　(LYS)　were　prepared　on　NCS　nanorods　by　spinning　coating　and　thermal　stabilization.　The　microstructure,　ion　releasing　and　wettability　of　coatings　were　observed;　osteoblast　and　endotheliocyte　behaviors,　as　well　as　bacteria　response　in　vitro,　were　evaluated.　The　protein　layer　did　not　change　microstructure　and　phase　composition　of　nanorods　but　acted　as　a　barrier　isolating　nanorods　from　contacting　medium,　and　it　results　in　slowing　down　the　degradation　of　nanorods.　The　reduced　increase　of　pH　value,　released　Ca　and　Si　ions　and　incorporation　of　LYS　in　BSA　protein　layer　gave　NCS　nanorods　improved　MC3T3-E1　and　HUVECs　response,　compared　with　Ti.　Meanwhile,　the　protein　layers　except　pure　LYS　endowed　nanorods　with　a　highly　antibacterial　effect　against　staphylococcus　aureus　and　escherichia　coli　simultaneously,　indicating　the　key　role　of　BSA　in　resisting　antibacterial　adhesion.　NCS　nanorods　with　BSA　and　LYS　composited　protein　layers　should　be　a　potential　coating　applied　on　Ti　implants　for　increasing　osseointegration.　©　2021