Infections　resulting　from　bacteria　and　biofilms　have　become　a　huge　problem　threatening　human　health.　In　recent　years,　the　antibacterial　and　antibiofilm　effects　of　graphene　and　its　derivatives　have　been　extensively　studied.　However,　there　continues　to　be　some　controversy　over　whether　graphene　and　its　derivatives　can　resist　infection　and　biofilms.　Moreover,　the　antibacterial　mechanism　and　cytotoxicity　of　graphene　and　its　derivatives　are　unclear.　In　the　present　review,　antibacterial　and　antibiofilm　abilities　of　graphene　and　its　derivatives　in　solution,　on　the　surface　are　reviewed,　and　their　toxicity　and　possible　mechanisms　are　also　reviewed.　Furthermore,　we　propose　possible　future　development　directions　for　graphene　and　its　derivatives　in　antibacterial　and　antibiofilm　applications.