Structural　biology　develops　rapidly　as　time　goes　on.　Based　only　on　static　structure　analysis　of　biomaterials　is　not　enough　to　satisfy　the　studies　of　their　functional　mechanisms,　with　a　huge　obstacle　for　the　dynamic　process　of　biological　complexes.　The　rapid　development　of　cryo-electron　microscopy(cryo-EM)　technology　makes　that　it　is　possible　to　observe　the　near-atomic　resolution　structures　and　dynamic　nature　of　biological　macromolecules,　in　the　fields　of　dynamic　characteristics　of　proteins,　protein-protein　interactions,　molecular　recognition,　and　structure-based　design.　In　this　review,　we　systematically　elaborate　the　contribution　of　cryo-EM　technology　in　the　field　of　biomaterials　such　as　ribosome　motion,　membrane　protein　structure　and　conformational　space,　dynamic　transmission　within　the　plasma　membrane　and　clinical　applications.　We　also　put　forwards　a　new　standpoint　in　the　development　of　cryo-EM　technology.