To　investigate　the　genetic　polymorphisms　of　59　Y-chromosomal　short　tandem　repeat　(Y-STR)　loci　in　the　Yulin　Han　population,　229　unrelated　healthy　male　individuals　were　analyzed　using　AGCU　Y37　kit　and　AGCU　Y-SUPP　Plus　kit.　A　total　of　227　different　haplotypes　were　obtained　at　the　59　Y-STR　loci.　Among　them,　225　haplotypes　were　unique　and　2　haplotypes　occurred　twice.　The　overall　haplotypic　diversity　and　discrimination　capacity　were　0.9999　and　0.9913,　respectively.　The　phylogenetic　relationships　between　the　studied　Yulin　Han　population　and　17　previously　reported　reference　populations　were　evaluated　via　multidimensional　scaling　and　Neighbor-Joining　analyses　based　on　the　haplotypic　frequencies　of　?YHRD　Maximal　Loci?.　Phylogenetic　analysis　revealed　that　Yulin　Han　population　was　closely　related　to　Chinese　Han　and　Hunan　Yao　populations.　These　results　demonstrated　that　the　59　Y-STR　loci　were　useful　for　forensic　applications　and　population　genetic　studies.