Objective:　Multivitamins　containing　Tween　80　can　cause　anaphylactoid　reactions.　The　objective　of　this　study　was　to　develop　a　new　lipid　emulsion　containing　13　fat-　and　water-soluble　vitamins　for　injection　(13V-LE)　that　were　simultaneously　dissolved　in　one　bottle　and　to　evaluate　the　stability　of　and　anaphylactoid　reactions　to　13V-LE.　Methods:　Particle　size,　zeta-potential,　and　polydispersity　of　13V-LE　were　assayed　with　a　Zetasizer　Nano　ZS.　Entrapment　efficiency　of　13V-LE　was　determined　with　HPLC.　Behavior,　histamine,　and　blood　pressure　of　beagle　dogs　were　investigated　by　observation,　fluorospectrophotometry,　and　sphygmomanometry.　Results:　The　13V-LE　with　the　smallest　particles　and　highest　entrapment　efficiency　with　stable　zeta-potential　was　composed　of　soybean　oil,　glycerin　(2.25%,　w:v),　egg　lecithin　(1.2%,　w:v),　and　purified　water.　There　was　no　obvious　change　in　characteristics　of　the　13V-LE　samples　in　terms　of　appearance,　size　distribution,　zeta-potential,　pH　value,　or　concentration　over　6　months.　In　anaphylactoid　reactions　tests,　when　being　administered　with　the　multivitamin　Infuvite　Adult　containing　Tween　80,　six　beagles　showed　grade　IV　symptoms　(P＜0.01　vs　control),　low　blood　pressure,　and　high　plasma-histamine　concentrations　(P＜0.05　or　P＜0.01).　However,　there　were　no　significant　differences　in　behavior,　blood　pressure,　or　histamine　concentration　in　the　dogs　before　and　after　administration　in　the　13V-LE　group.　Conclusion:　The　13V-LE　formulation　is　a　suitable　intravenous　lipid　emulsion　without　anaphylactoid　reactions.