Extrusion-based　printing　technology　has　a　promising　development　in　the　field　of　tissue　engineering　scaffold.　Researchers　package　cells/particles　in　biomaterials　and　then　extrude　them　through　a　nozzle　and　solidify　to　get　a　scaffold.　This　method　can　achieve　uniform　distribution　of　cells/particles　in　biomaterials　or　the　addition　of　particles　in　biomaterials　to　enhance　the　mechanical　properties　of　the　scaffold.　Sodium　alginate　is　a　kind　of　polysaccharide　biomaterial　which　has　a　wide　range　of　applications　in　the　field　of　bioprinting.　However,　the　diameter　of　the　nozzle　determines　the　accuracy　of　the　cells/particles　position　in　the　alginate　scaffold.　A　method　of　ultrasonic-assisted　3D　bioprinting　a　composite　of　alginate　and　particles　is　proposed.　The　acoustic　pressure　can　concentrate　cells/particles　to　the　center　of　the　nozzle,　which　can　achieve　a　more　precise　positioning　than　the　diameter　of　the　nozzle,　and　facilitate　the　purpose　of　depositing　specific　cells/particles　to　the　determined　location　of　the　biomaterial.　©　2021　Institute　of　Physics　Publishing.　All　rights　reserved.