Measurement　of　mucosal　wave　velocity　on　the　vocal　fold　surface　is　important　and　useful　in　investigation　of　vocal　fold　tissue　mechanics　and　diagnosis　of　vocal　fold　diseases.　In　this　work,　Laser　Doppler　Vibrometer　(LDV　was　used　to　measure　the　vibration　of　the　superior　surface　of　the　vocal　fold,　finally　reconstructing　the　shape　variation　of　the　superior　surface　and　estimating　the　mucosal　wave　velocity　through　the　electroglottography-based　time-domain　synchronization.　In　the　experiment　of　excised　canine　vocal　fold　vibration,　the　mucosal　wave　velocities　estimated　using　LDV　were　compared　equally　to　those　measured　by　high-speed　video.　Additionally,　the　method　is　able　to　capture　the　shape　changing　of　the　superior　surface　in　high　time　resolution.　However,　because　of　the　limit　in　single　point　measurement　and　time　synchronization,　the　vocal　fold　vibration　should　be　stable　during　the　measurement　to　reduce　the　measurement　error.　©　2020　Acta　Acustica.