Laryngeal　squamous　cell　carcinoma　(LSCC)　is　an　aggressive　malignancy　with　highly　mortality　rate.　Long　non-coding　RNA　(lncRNA)　AGAP2-AS1　is　an　identified　oncogene　in　several　types　of　cancers.　However,　the　role　of　AGAP2-AS1　in　LSCC　remains　unclear.　The　expression　levels　of　AGAP2-AS1　in　LSCC　tissues　and　cell　lines　were　measured　using　qRT-PCR.　AGAP2-AS1　was　knocked　down　in　LSCC　cells　through　transfection　with　siRNA-AGAP2-AS1.　Cell　proliferation　and　invasion　were　detected　using　MTT　and　transwell　assays.　Dual-luciferase　reporter　gene　assay　was　performed　to　confirm　the　interaction　with　AGAP2-AS1　and　downstream　genes.　Our　results　showed　that　AGAP2-AS1　expression　was　remarkably　increased　in　human　LSCC　tissues　and　cell　lines.　Knockdown　of　AGAP2-AS1　significantly　inhibited　the　proliferation　and　invasion　of　LSCC　cells.　In　addition,　AGAP2-AS1　sponged　miR-193a-3p　and　regulated　its　expression　in　LSCC　cells.　Inhibition　of　miR-193a-3p　reversed　the　effects　of　AGAP2-AS1　knockdown　on　LSCC　cells.　Furthermore,　Lysyl　oxidase-like　4　(LOXL4)　was　a　target　gene　of　miR-193a-3p　and　the　role　of　miR-193a-3p　was　mediated　by　LOXL4.　In　conclusion,　these　findings　suggest　that　knockdown　of　AGAP2-AS1　inhibited　the　proliferation　and　invasion　of　LSCC　cells　through　regulating　the　miR-193a-3p/LOXL4　axis.