Microfluidic　mixing　is　a　critical　issue　in　the　lab-on-a-chip　system,　which　has　been　applied　in　various　biomedical　research.　Recently,　acoustic　wave-based　active　micromixers　have　been　applied　to　increase　the　mixing　efficiency　via　acoustic　streaming.　However,　along　with　the　acoustic　streaming,　the　acoustic　thermal　effect　will　generate　additional　heat　to　increase　the　temperature　of　the　microfluid.　In　this　work,　we　present　an　integrated　thermal　control　unit　applied　to　a　surface　acoustic　wave　(SAW)-assisted　micromixer　to　address　this　problem.　A　metal　microwire　as　the　thermal　resistance　sensor　is　fabricated　under　the　microchannel　to　measure　the　temperature　in　different　conditions,　together　with　the　Peltier　cooler　beneath　allowing　the　active　temperature　control　of　the　microfluid.　For　characterization,　neutralization　reaction　and　alkaline　phosphatase　assay　were　successfully　conducted　on　this　device.　It　shows　the　integrated　thermal　control　unit　applied　to　a　SAW-assisted　micromixer　is　highly　effective　in　temperature-sensitive　biomedical　research.　©　2022　Elsevier　B.V.