Recently,　Ganoderma　lucidum　spores　(GLS)　have　shown　anti-epileptic　effects.　However,　there　are　no　reports　on　the　anti-epileptic　effects　of　its　chemical　constituents　ganoderic　acids　(GAs),　and　more　research　is　needed　to　better　understand　the　mechanism　of　GLS　activity.　In　this　work,　rat　primary　hippocampal　neurons　in　an　in　vitro　model　were　used　to　assess　the　intervention　effects　of　GAs　on　epileptiform　discharge　hippocampal　neurons　and　expression　of　both　BDNF　and　TRPC3,　with　the　aid　of　immunofluorescence,　MTT　method　and　flow　cytometry.　It　was　found　that　BDNF　and　TRPC3　are　expressed　in　all　cells　and　were　mainly　localized　in　the　cytoplasm.　The　fluorescence　intensities　of　BDNF　and　TRPC3　in　GAs　groups　were　higher　than　those　of　normal　control　and　model　groups,　especially　at　80　mu　g/ml　(P＜0.05).　The　apoptosis　rate　of　neurons　was　inversely　proportional　to　BDNF　and　TRPC3　changes　(P＜0.01).　Therefore,　BDNF　and　TRPC3　should　be　involved　in　the　occurrence　and　development　of　epilepsy.　GAs　might　indirectly　inhibit　mossy　fiber　sprouting　and　adjust　the　synaptic　reconstructions　by　promoting　the　expression　of　BDNF　and　TRPC3.　Besides,　GAs　could　exert　a　protective　effect　on　hippocampal　neurons　by　promoting　neuronal　survival　and　the　recovery　of　injured　neurons.