Transplantation　of　cryopreserved　ovarian　tissue　has　been　considered　as　a　promising　way　of　fertility　preservation　for　women.　however,　this　cryopreservation　method　is　prone　to　post-resuscitation　follicle　proliferation　and　oocyte　development　stagnation,　affecting　late　transplant　survival.　To　evaluate　current　vitrification　works,　we　investigated　the　critical　pathway　alternations　in　vitrified-warmed　juvenile　10-day-old　mouse　ovary.　We　showed　a　significant　decrease　of　protein　kinase　B　(Akt)　and　Mitogen-activated　protein　kinase　(Mapk)　phosphorylation,　during　which　serine/threonine　kinases　play　central　roles　in　coordinating　follicle　and　oocyte　development　and　stress　response.　Inhibition　of　Akt　and　Mapk　activity　were　associated　with　one　of　the　imprinted　insulin　pathway　negative　regulatory　genes,　Growth　factor　receptor-binding　protein　10　(Grb10)　which　remarkably　increased　in　vitrified-warmed　juvenile　mouse　ovary　than　that　of　fresh　group　(p　＜　0.05).　RNAi-induced　Grb10　down-regulation　reversed　the　decrease　in　Akt　and　Mapk　phosphorylation.　The　increase　of　GrblO　expression　was　partially　caused　by　the　hyper-methylation　of　the　promoter　region,　associated　with　the　decrease　of　follicular　DNA　methyltransferase　(Dnmt)　1　protein　in　different　stages　of　vitrified-warmed　group,　compared　to　fresh　group　(p　＜　0.05).　The　mRNA　and　protein　expression　of　Dnmt1　in　ovary　of　vitrified-warmed　juvenile　mouse　were　remarkably　lower　than　those　in　fresh　group　(p　＜　0.05).　Dnmtl　overexpression　dramatically　reversed　GrblO　up　regulation　and　Akt　and　Mapk　phosphorylation　reduction.　Taken　together,　our　findings　suggest　that　GrblO　expression　might　be　helpful　in　evaluation　of　effectiveness　of　vitrification,　and　considered　as　a　potential　target　for　further　vitrification　protocols　improvement　in　the　future.