The　aim　of　the　present　study　was　to　evaluate　the　effects　of　passage　and　cryopreservation　of　choroid　plexus　epithelial　cells　on　their　secretion　of　neurotrophic　factors.　Choroid　plexus　epithelial　cells　were　cryopreserved　and　thawed　following　primary　culture　or　passage　cultured　for　up　to　two　passages.　The　supernatant　of　primary,　first/second　passage　and　cryopreserved-thawed　choroid　plexus　epithelial　cells　was　collected　when　cells　reached　80-90%　confluence.　ELISA　was　used　to　quantify　brain-derived　neurotrophic　factor　(BDNF),　glial　cell-derived　neurotrophic　factor　(GDNF),　nerve　growth　factor　(NGF)　and　ciliary　neurotrophic　factor　(CNTF)　levels　in　the　cell　supernatant.　First　passage　and　cryopreserved-thawed　cells　secreted　less　BDNF　and　CNTF　compared　with　primary　cultured　cells　and　increased　levels　of　these　two　factors　compared　with　second　passage　cells,　and　increased　levels　of　GDNF　and　NGF　compared　with　primary　cultured　and　second　passage　cells　(all　P＜0.05).　Therefore,　first　passage　culture　decreased　BDNF　and　CNTF　secretion　but　increased　NGF　and　GDNF　compared　with　primary　culture;　second　passage　culture　diminished　neurotrophic　factor　secretion　compared　with　first　passage　culture;　and　cryopreservation　did　not　weaken　the　function　of　choroid　plexus　epithelial　cells　in　secreting　BDNF,　GDNF,　NGF　and　CNTF.　The　current　study　demonstrates　that　first　passage　and　cryopreserved-thawed　choroid　plexus　epithelial　cells　have　an　enhanced　function　to　secrete　neurotrophic　factors　including　BDNF,　GDNF,　NGF　and　CNTF.