Based　on　static　loading　test　analysis,　the　tensile-compressive　fatigue　tests　of　wood　in　ancient　buildings　were　carried　out　using　the　MTS858　machine　with　a　frequency　of　2　Hz.　The　result　shows　that　most　specimens　failure　along　the　veins　of　wood.　The　stress-strain　and　deformation　characteristics　of　the　specimens　were　analyzed.　The　stress-strain　curves　become　sparse　gradually　and　the　tensile-strain　become　significant　due　to　the　increase　of　upper　stress　radio.　The　deformation　growth　of　the　specimen　can　be　divided　into　three　phases　similar　to　the　development　of　fatigue　deformation　and　damage　of　material,　namely,　the　occurrence　of　damage,　the　stable　propagation　of　damage　and　the　failure.　S-N　relationship　of　wood　sample　is　plotted　with　an　index　function　fitted.　The　upper　limit　of　the　stress　ratio　of　ancient　wood　is　suggested　to　be　0.30.　©　2016,　Engineering　Mechanics　Press.　All　right　reserved.