©,　2015,　Journal　of　Xi＇an　Jiaotong　University　(Medical　Sciences).　All　right　reserved.Objective:　To　obtain　a　large　number　of　recombinant　adenoviruses　of　PPARγ　(Ad/PPARγ)　and　MED1　(Ad/MED1)　and　investigate　the　biological　function　of　nuclear　receptor　PPARγ　and　its　coactivator　mediator　1(MED1).　Methods:　HEK293a　cell　line　was　amplified　and　used　to　package　the　adenovirus　stocks　(Ad/PPARγ　or　Ad/MED1).　CsCl　gradient　centrifuge　was　used　to　purify　the　adenovirus,　and　virus　particles　were　determined　by　A＜inf＞260＜/inf＞.　After　cell　infection　or　mouse　injection　with　adenovirus,　the　biological　function　of　Ad/PPARγ　and　Ad/MED1　was　analyzed　using　HE　staining,　Real-time　PCR　or　immunohistochemistry.　Results:　We　obtained　control　Ad/LacZ,　Ad/PPARγ　and　Ad/MED1,　whose　concentrations　were　2.51×10＜sup＞12＜/sup＞　VP/mL,　1.57×10＜sup＞12＜/sup＞　VP/mL　and　2.59×10＜sup＞12＜/sup＞　VP/mL,　respectively.　Ad/PPARγ　injected　into　the　tail　vein　led　to　hepatic　steatosis　in　C57BL/6J　mice.　HE　and　oil　red　O　staining　results　showed　that　many　lipid　droplets　accumulated　in　hepatocytes.　Real　time　PCR　and　immunohistochemistry　showed　that　the　expressions　of　PPARγ　mRNA　and　protein　were　remarkably　increased.　Also,　MED1　mRNA　expression　was　significantly　increased　in　3T3-L1　cell　line　or　C57BL/6J　mice　after　Ad/MED1　treatment　compared　with　that　in　control　group.　Conclusion:　Ad/PPARγ　and　Ad/MED1　were　prepared　successfully,　which　provides　materials　for　cell　infection,　especially　for　gene　function　and　network　regulation　in　vivo.