Suspension　array　is　a　promising　platform　for　multiplex　analyses　with　broad　applications　in　science　research　and　clinical　diagnosis.　As　one　of　the　key　components　for　the　suspension　array,　fluorescence-encoded　microspheres　have　received　much　attention　and　a　lot　of　achievements　have　been　made.　In　this　review,　the　analysis　pattern　and　applications　of　suspension　array　were　introduced,　and　recent　advances　in　the　fluorescence-encoded　microspheres　were　summarized　according　to　the　classification　of　preparation　methods　including　swelling　method,　layer-by-layer　(LBL)　self-assembly,　embedding　in　microspheres,　microfluidic　techniques　and　membrane　emulsification.　Current　challenges　and　future　directions　of　fluorescence-encoded　microspheres　were　also　outlined.