©　2018,　Scientific　Publishers　of　India.　All　rights　reserved.Objective:　To　investigate　the　effect　of　the　Zhikang　capsule　on　the　growth　and　apoptosis　of　breast　cancer　cell　line　MCF-7.　Method:　MTT　method　was　used　to　detect　the　growth　situation　of　normal　breast　cancer　MCF-7　cells　at　24　h,　48　h　and　72　h　after　treatment　by　Zhikang　capsule　and　the　effects　of　Zhikang　capsule　on　cell　cycle　of　MCF-7　cells　were　detected　by　flow　cytometry　using　flow　cytometer.　The　effects　of　24　h,　48　h　and　72　h　after　treatment　by　Zhikang　capsule　on　the　growth　of　normal　breast　cancer　MCF-7　cells　were　measured　by　flow　cytometry;　the　effect　of　Zhikang　capsule　on　apoptosis　of　MCF-7　cells　was　detected　by　PI　single　staining　flow　cytometry.　Result:　Different　concentrations　of　Zhikang　capsule　could　effectively　inhibit　the　growth　of　MCF-7　cells,　and　with　the　increase　of　concentration　and　extension　of　action　time,　the　cell　growth　inhibition　rate　increased.　The　MCF-7　cells　were　effect　for　72　h　by　the　0.5,　1,　2.0　mg/ml　of　the　Zhikang　capsule,　and　the　proportion　of　G0/G1　phase　cells　in　cell　cycle　was　19.33　±　10.38%,　14.12　±　5.37%　and　26.84　±　2.13%;　While　the　control　group　G0/G1　phase　ratio　was　51.83　±　1.90%.　The　apoptosis　rate　of　the　cells　were　2.09　±　0.74%,　3.84　±　0.78%　and　5.35　±　0.83%　respectively　treated　by　the　0.5,　1,　2.0　mg/ml　of　the　Zhikang　capsule.　Conclusion:　The　Zhikang　capsule　could　inhibit　the　growth　of　MCF-7　cells　in　vitro,　and　the　inhibitory　effect　showed　the　time　effect　and　dose　effect　relationship;　and　it　could　promote　the　apoptosis　of　breast　cancer　cells　by　blocking　the　cell　cycle　in　the　G2/M　phase.