Silk　fibroin　(SF)/polyvinyl　alcohol　(PVA)　composite　hydrogel　and　SF/PVA/hydroxyapatite　(HA)　composite　were　prepared　by　two　methods:　drying　at　room　temperature　and　repeated　freezing–thawing　cycles.　A　comparative　analysis　was　carried　out　for　the　composite　material　under　various　conditions,　focusing　on　the　properties　of　SF/PVA/HA　composites,　studied　by　Fourier　transform　infrared,　scanning　electron　microscope,　swelling　characteristics,　mechanical　compression,　cytotoxicity,　in　vitro　degradation,　and　drug　carrying　release　experiment.　The　results　show　that　compared　with　the　SF/PVA/HA　composites　prepared　by　cooled　at　room　temperature　method,　SF/PVA　composite　hydrogel　was　heated　at　80°C　after　repeated　freezing–thawing　for　three　times,　with　HA　blended,　the　SF/PVA/HA　composites　were　obtained　after　freeze-dried.　The　content　of　β-sheet　structure　noticeably　increased,　which　has　the　better　mechanical　properties　and　aqueous　solution　stability,　good　cell　compatibility,　moderate　in　vitro　degradation　rate　and　stable　drug　release　rate,　and　can　also　be　beneficial　for　material　used　in　drug-loaded　tissue　engineering　bone　scaffold　to　improve　degradation　of　uniform　rate　and　drug　release　stability.　It　provides　a　significant　technique　to　achieve　the　treatment　of　anti-bone　tuberculosis　and　lesion　bone　defect　repair.　POLYM.　COMPOS.,　2018.　©　2018　Society　of　Plastics　Engineers.　©　2018　Society　of　Plastics　Engineers