Serum　starvation　is　widely　used　in　cell　biology　to　trigger　cell　cycle　arrest,　apoptosis,　autophagy,　and　metabolic　adaptations.　Serum　starvation-related　molecular　events　have　been　well　characterized　at　protein　level　but　not　at　transcript　level:　how　long　non-coding　RNAs　contribute　to　the　regulation　of　protein-coding　genes　is　largely　unknown.　Here,　we　captured　the　IncRNA　transcriptome　in　serum　starved　mouse　embryonic　fibroblasts　and　identified　three　main　modes　of　action:　cis-acting/coregulatory,　trans-acting,　and　＂miRNA-carrier＂.　Whole-genome　and　individual　gene　level　analyses　support　that　our　annotation　provides　an　important　platform　for　understanding　lncRNA/protein-coding　gene　coregulatory　mechanisms　in　serum　starvation.