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Abstract:
Keshan disease (KD) is an endemic dilated cardiomyopathy with unclear etiology. In this study, we compared mitochondrial-related gene expression profiles of peripheral blood mononuclear cells (PBMCs) derived from 16 KD patients and 16 normal controls in KD areas. Total RNA was isolated, amplified, labeled and hybridized to Agilent human 4 x 44k whole genome microarrays. Mitochondrial-related genes were screened out by the Third-Generation Human Mitochondria-Focused cDNA Microarray (hMitChip3). Quantitative real-time PCR, immunohistochemical and biochemical parameters related mitochondrial metabolism were conducted to validate our microarray results. In KD samples, 34 up-regulated genes (ratios >= 2.0) were detected by significance analysis of microarrays and ingenuity systems pathway analysis (IPA). The highest ranked molecular and cellular functions of the differentially regulated genes were closely related to amino acid metabolism, free radical scavenging, carbohydrate metabolism, and energy production. Using IPA, 40 significant pathways and four significant networks, involved mainly in apoptosis, mitochondrion dysfunction, and nuclear receptor signaling were identified. Based on our results, we suggest that PGC-1 alpha regulated energy metabolism and anti-apoptosis might play an important role in the compensatory mechanism of MD. Our results may lead to the identification of potential diagnostic biomarkers for MD in PBMCs, and may help to understand the pathogenesis of MD. (C) 2013 Elsevier Inc. All rights reserved.
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EXPERIMENTAL CELL RESEARCH
ISSN: 0014-4827
Year: 2013
Issue: 17
Volume: 319
Page: 2604-2616
3 . 3 7 2
JCR@2013
3 . 9 0 5
JCR@2020
ESI Discipline: MOLECULAR BIOLOGY & GENETICS;
ESI HC Threshold:420
JCR Journal Grade:2
CAS Journal Grade:3
Cited Count:
WoS CC Cited Count: 9
SCOPUS Cited Count: 10
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 2