Context:　Coptidis　Rhizoma-Euodiae　Fructus　couple　(CEC)　is　a　classic　traditional　Chinese　medicine　preparation　consisting　of　Coptidis　Rhizoma　and　Euodiae　Fructus　at　the　ratio　of　6:　1,　and　used　to　treat　gastro-intestinal　disorders.　Alkaloids　are　the　main　bioactive　component.　This　research　provides　comprehensive　analysis　information　for　the　quality　control　of　CEC.　Objective:　To　develop　a　high-performance　liquid　chromatography-diode　array　detection　fingerprint　for　chemical　composition　characteristics　of　CEC　and　its　products.　Materials　and　methods:　The　samples　were　separated　with　a　Gemini　C-18　column　by　using　gradient　elution　with　water-formic　acid　(100:0.03)　and　acetonitrile　as　mobile　phase.　Flow　rate　was　1.0　mL/min　and　detection　wavelength　was　250　nm.　Similarity　analysis　and　principal　component　analysis　(PCA)　were　employed　to　evaluate　quality　consistencies　of　analytes.　Mean　chromatograms　and　correlation　coefficients　of　analytes　were　calculated　by　the　software　＂Similarity　Evaluation　System　for　Chromatographic　Fingerprint　of　Traditional　Chinese　Medicine＇＇.　Results:　Fingerprint　chromatogram　comparison　determined　20　representative　general　fingerprint　peaks,　and　the　fingerprint　chromatogram　resemblances　are　all　better　than　0.988.　Consistent　results　were　obtained　to　show　that　CEC　and　its　related　samples　could　be　successfully　divided　into　three　groups.　Contribution　plots　generated　by　PCA　were　performed　to　interpret　differences　among　the　sample　groups　while　peaks　which　significantly　contributed　to　classification　were　identified.　Seven　bioactive　constituents　in　the　samples　were　verified　by　quantitative　analysis.　Discussion　and　conclusion:　The　chromatographic　fingerprint　with　similarity　evaluation　and　PCA　assay　combined　with　quantification　of　seven　compounds　could　be　utilized　as　a　quality　control　method　for　the　herbal　couple.