Thermo-stable　hollow　magnetic　microspheres　are　prepared　by　layer-by-layer　(LbL)　assembly　on　a　uniform　melamine-formaldehyde　resin　microsphere　template　coated　by　silica　and　calcination　for　burning　off　organic　resin.　Trypsin　is　immobilized　by　their　coated　polydopamine　layer,　and　nanogold　particles　are　assembled　onto　the　microspheres　by　the　LbL　method　for　catalytic　applications.　The　morphologies　and　hollow　structures　are　observed　by　using　a　scanning　electron　microscope　and　transmission　electron　microscope.　The　characteristics　are　investigated　by　Fourier　transfer　infrared　spectroscopy,　thermo-gravimetric　analysis　and　vibrating　sample　magnetometry.　Meanwhile,　the　catalytic　characteristics　of　nanogold　particles　and　trypsin　are　evaluated　by　ultraviolet-visible　spectra.　The　prepared　hollow　thermostable　magnetic　microspheres　are　narrow-dispersed　with　a　relatively　low　coefficient　of　variation,　and　their　size　ranging　from　0.5　mu　m　to　5.0　mu　m　and　saturation　magnetization　(4-30　emu　g(-1))　can　be　regulated　by　different　conditions.　Combined　with　magnetic　separation　and　calcination　activation　or　regeneration,　these　microspheres　can　also　be　reused　over　20　times　for　nanogold　catalyst　and　over　32　times　for　trypsin　catalyst　reconjugation　without　any　significant　loss　in　catalytic　activity,　indicating　their　considerable　potential　for　recyclable　catalytic　applications.