Lentiviral　vector　infection　of　enhanced　green　fluorescent　protein　fluorescence　reporter　genes　in　enteric　neural　crest-derived　cells　maintained　efficient,　stable,　long-term　labeling　and　the　infected　enteric　neural　crest-derived　cells　could　survive,　proliferate,　and　express　fluorescent　reporter　genes.　However,　the　method　does　not　show　whether　there　is　some　defined　or　undefined　toxicity　to　the　enteric　neural　crest-derived　cells,　which　may　affect　enteric　neural　crest-derived　cells＇　properties.　Here,　we　evaluated　the　enteric　neural　crest-derived　cells　properties　under　the　influence　of　lentivirus　infection　of　enhanced　green　fluorescent　protein　fluorescence　reporter　genes.　This　study　used　the　cell　count　kit-8　for　measurement　of　vitality,　transwell　for　cell　migration,　immunocytochemistry　for　cell　count　and　identification,　and　tested　the　apoptosis　of　the　enteric　neural　crest-derived　cells　with　flow　cytometry.　The　enteric　neural　crest-derived　cells　with　or　without　lentivirus　and　their　derivative　enteric　neural　crest-derived　cells　could　form　characteristic　neurospheres,　and　maintain　their　level　of　fluorescent　label　steady.　When　cultured　under　inducing　conditions,　enteric　neural　crest-derived　cells　differentiated　into　neurons　and　glia.　The　results　showed　that　the　enteric　neural　crest-derived　cells　with　or　without　lentivirus　showed　no　significant　difference　in　viability,　migration,　apoptosis,　neuronal,　and　glial　ratio.　The　study　identified　that　lentivirus　can　be　used　in　a　nontoxic　manner　for　infection　of　enhanced　green　fluorescent　protein　fluorescence　reporter　genes　into　enteric　neural　crest-derived　cells.