Background:　Different　single　nucleotide　polymorphisms　(SNPs)　in　gene　may　lead　to　different　drug　metabolic　activity,　and　different　SNPs　may　also　be　associated　with　genetic　predisposition　of　certain　diseases.　CYP3A5,　as　one　of　the　most　active　gene　in　CYP450　3A　superfamily,　has　become　a　hot　topic　in　medicine.　Since　the　research　about　the　genetic　diversity　of　CYP3A5　in　Tibetans　is　rare,　we　carried　the　study　to　explore　the　genetic　polymorphisms　of　it　in　one　Tibetan　population　who　living　in　Tibetan.　Methods:　DNA　sequencing　the　promoter,　exons　and　surrounding　introns,　and　3＇-untranslated　region　of　the　CYP3A5*1　gene　in　96　healthy,　unrelated　volunteers　of　Tibetan　by　Direct　Sequencing　approach.　Results:　We　finally　identified　23　different　CYP3A5　polymorphisms,　including　three　novel　mutants　in　promoter,　one　novel　nonsynonymous　variant　(C27122A)　and　four　in　the　intron　region.　We　found　the　allele　frequencies　of　CYP3A5*1A,　CYP3A5*1C,　CYP3A5*1E　and　CYP3A5*3A　were　0.328,　0.52,　0.182　and　0.484,　respectively.　Tfsitscan　analyzed　that　some　mutants　leads　the　density　of　transcription　factor　binding　varied　in　the　different　promoter　regions.　Conclusion:　Our　results　obtain　basic　information　of　CPY3A5　alleles　in　Tibetans　and　suggest　that　the　diverse　ethnic　populations　may　have　different　enzymatic　activities　of　CYP3A5.