The　aim　of　this　study　was　to　elucidate　the　linear　and　non-linear　responses　of　ultrasound　contrast　agent　(UCA)　to　frequency-dispersive　guided　waves　from　the　tibia　cortex,　particularly　two　individual　modes,　S0　(1.23　MHz)　and　A1　(2.06　MHz).　The　UCA　responses　to　guided　waves　were　illustrated　through　the　Marmottant　model　derived　from　measured　guided　waves,　and　then　verified　by　continuous　infusion　experiments　in　a　vessel-tibia　flow　phantom.　These　UCA　responses　were　further　evaluated　by　the　enhanced　ratio　of　peak　values　and　the　resolutions　of　UCA　backscattered　echoes.　Because　of　the　individual　modes　S0　and　A1　in　the　tibia,　the　peak　values　of　the　UCA　backscattered　echoes　were　enhanced　by　83.57　+/-　7.35%　(p　＜　0.05)　and　80.77　+/-　6.60%　(p　＜　0.01)　in　the　UCA　subharmonic　frequency　and　subharmonic　imaging,　respectively.　However,　corresponding　resolutions　were　0.78　+/-　0.07　(p　＜　0.05)　and　0.72　+/-　0.12　(p　＜　0.01)　times　those　without　guided　wave　disturbances,　respectively.　Even　though　the　resolution　was　partly　degenerated,　the　subharmonic　detection　sensitivity　of　UCA　was　improved　by　the　guided　waves.　Thus,　UCA　responses　to　the　double-frequency　guided　waves　should　be　further　explored　to　benefit　the　detection　of　capillary　perfusion　in　tissue　layers　near　the　bone　cortex,　particularly　for　perfusion　imaging　in　the　free　flaps　and　skeletal　muscles.　(C)　2016　World　Federation　for　Ultrasound　in　Medicine　&　Biology.