We　investigated　the　associations　between　single　nucleotide　polymorphisms　(　SNPs)　in　the　testis-specific　Y-encoded-like　protein　6　(　TSPYL6)　gene　and　breast　cancer　(　BC)　susceptibility　in　the　Han　Chinese　population.　A　total　of　183　BC　patients　and　195　healthy　women　were　included　in　the　study.　Six　SNPs　in　TSPYL6　were　genotyped　and　the　association　with　BC　risk　analyzed.　Odds　ratios　(　ORs)　and　95%　confidence　intervals　(　95%　CIs)　were　calculated　using　unconditional　logistic　regression　analysis.　Multivariate　logistic　regression　analysis　was　used　to　identify　SNPs　that　correlated　with　BC　susceptibility.　Rs11896604　was　associated　with　a　decreased　risk　of　BC　based　on　dominant　and　genotype　models.　Rs843706　was　associated　with　an　increased　risk　of　BC　based　on　a　recessive　model.　Rs11125529　was　associated　with　decreased　BC　susceptibility　based　on　a　genotype　model.　Finally,　rs843711　inversely　correlated　with　clinical　stage　III/IV　BC.　Our　findings　reveal　a　significant　association　between　SNPs　in　the　TSPYL6　gene　and　BC　risk　in　a　Han　Chinese　population.