Chemical　stimulation　of　white　adipose　tissue　(WAT)　causes　adipose　afferent　reflex　(AAR)　and　sympathetic　activation.　This　study　is　to　investigate　the　effects　of　AAR　on　lipolysis　and　the　mechanisms　of　attenuated　lipolysis　response　to　enhanced　AAR　in　obesity.　Obesity　was　caused　by　high-fat　diet　for　12　weeks　in　rats.　AAR　was　induced　by　injection　of　capsaicin　into　inguinal　WAT　or　electrical　stimulation　of　epididymal　WAT　afferent　nerve.　AAR　caused　sympathetic　activation,　which　was　enhanced　in　obesity　rats.　AAR　increased　cAMP　levels　and　PKA　activity,　promoted　hormone　sensitive　lipase　(HSL)　and　perilipin　phosphorylation,　and　increased　lipolysis　in　WAT,　which　were　attenuated　in　obesity　rats.　PKA　activity,　cAMP,　perilipin　and　beta-adrenoceptor　levels　were　reduced,　while　HSL　was　upregulated　in　adipocytes　from　obesity　rats.　In　primary　adipocytes,　isoproterenol　increased　cAMP　levels　and　PKA　activity,　promoted　HSL　and　perilipin　phosphorylation,　and　increased　lipolysis,　which　were　attenuated　in　obesity　rats.　The　attenuated　effects　of　isoproterenol　in　adipocytes　from　obesity　rats　were　prevented　by　a　cAMP　analogue　dbcAMP.　The　results　indicate　that　reduced　lipolysis　response　to　enhanced　AAR　in　obesity　is　attributed　to　the　impaired　activation　of　beta-adrenoceptor-cAMP-PKA-HSL　pathway.　Increased　cAMP　level　in　adipocytes　rectifies　the　attenuated　lipolysis　in　obesity.