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Abstract:
Context: Gastric ulcer is a common gastrointestinal disorder with increasing incidence and prevalence attributed to loss of balance between aggressive and protective factors. Nobiletin (NOB), a major component of polymethoxyflavones in citrus fruits, has a broad spectrum of health beneficial properties including anti-inflammatory and anti-tumor activities. Although NOB was originally shown to possess anti-inflammatory activity, its effects on gastric ulcer were rarely explored previously. Objective: The aim of the present study was to investigate the anti-ulcerogenic activity of NOB on ethanol-induced gastric ulcer in mice and to elucidate the underlying mechanisms. Methods: Seventy-two male Kunming mice administered with absolute ethanol (0.2ml/animal) were pretreated with NOB (5, 10 or 20mg/kg), cimetidine (100mg/kg), or vehicles by intragastric administration in different experimental groups for three days, and animals were euthanized 3h after ethanol ingestion. Gross and microscopic lesions, immunological and biochemical parameters were taken into consideration. Results: The results showed that ethanol induced gastric injury, increased malondialdehyde (MDA) levels, decreased glutathione (GSH) content, superoxide dismutase (SOD) activity, and prostaglandin E-2 (PGE(2)) levels, increased pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-) and interleukin-6 (IL-6) levels and myeloperoxidase (MPO) activity, as well as the expression MAPK signaling pathway. Pretreatment with NOB significantly attenuated the gastric lesions as compared to the ethanol group. Conclusions: These findings suggest that the gastroprotective activity is attribute to the improvement of antioxidant activities, the stimulation of PGE(2), and the reduction of pro-inflammatory cytokines through the MAPK pathway.
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IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
ISSN: 0892-3973
Year: 2017
Issue: 6
Volume: 39
Page: 354-363
2 . 0 2 4
JCR@2017
2 . 7 3 0
JCR@2020
ESI Discipline: IMMUNOLOGY;
ESI HC Threshold:178
JCR Journal Grade:4
CAS Journal Grade:4
Cited Count:
WoS CC Cited Count: 29
SCOPUS Cited Count: 42
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 5