Approximately　10%　of　infantile　hemangiomas　(IHs)　are　the　most　common　vascular　tumors　affecting　children　and　are　characterized　by　rapid　growth,　and　can　have　destructive,　disfiguring　and　even　life-threatening　consequences.　Currently,　propranolol　is　considered　to　be　a　safe　and　effective　treatment　option　for　problematic　proliferating　IHs.　Recent　studies　have　also　revealed　that　microRNAs　(miRNAs　or　miRs)　play　important　roles　in　the　regulation　of　angiogenesis.　In　this　study,　XPTS-1　cells　were　used　as　a　hemangioma-derived　endothelial　cell　line　constructed　in　our　laboratory.　Through　a　series　of　experiments,　we　discovered　that　miR-382　is　a　novel　miRNA　associated　with　IHs,　which　was　overexpressed　in　XPTS-1　cells　and　was　conversely　downregulated　by　treatment　with　propranolol.　In　addition,　we　found　that　miR-382　contributes　to　the　progression　of　IHs.　Our　results　revealed　that　propranolol　inhibited　XPTS-1　cell　migration　and　proliferation,　and　promoted　apoptosis,　and　these　effects　were　reversed　by　the　restoration　of　miR-382　expression　by　transfection　of　the　cells　with　an　miR-382　overexpression　vector.　Further　experiments　revealed　that　the　above-mentioned　effects　were　associated　with　the　phosphatase　and　tensin　homolog　(PTEN)-mediated　AKT/mammalian　target　of　rapamycin　(mTOR)　signaling　pathway.　The　expression　of　PTEN　was　upregulated,　while　that　of　p-AKT,　p-mTOR　and　p-p70S6K　was　downregulated　by　propranolol;　these　effects　were　partly　reversed　by　the　overexpression　of　miR-382.　On　the　whole,　our　study　identified　that　the　downregulation　of　miR-382　by　propranolol　inhibits　the　progression　of　IHs　via　the　PTENmediated　AKT/mTOR　pathway.