A　novel　protocol　is　developed　to　prepare　quantum　dot　(QD)/Ir(III)　complex　glycerol　monoolein　(GMO)　cubic-phase　nanoparticles　(Qd/Ir　GMCPNPs)　as　hypoxia　nanoprobes,　in　which　hypoxia　probe　Tris　[1-phenylisoquinoline-C2,　N]　Iridium(III)　[Ir(piq)(3)]　and　the　reference　QDs　are　separately　loaded　at　hydrophilic　and　hydrophobic　channels　to　avoid　interference.　Qd/Ir　GMCPNPs　were　nearly　spherical　in　shape,　with　an　average　size　of　20-30　nm.　Their　phosphorescence　spectra　showed　that　nanoprobes　have　a　wide　excited　wave　length　range　of　360-500　nm,　which　is　suitable　for　different　types　of　measurement　instruments.　When　the　oxygen　content　decreased　from　21%　to　1%,　the　luminescent　intensity　ratio　of　Qd/Ir　GMCPNPs　in　the　solution　and　cells　increased　4-fold　and　2.8-fold,　respectively,　with　an　acceptable　linear　relationship.　Particularly,　extensive　preliminary　quantitative　ratiometric　oxygen　sensing　and　long　tumor　imaging　monitoring　can　be　achieved　with　these　nanoprobes.