In　this　study,　a　novel　method　utilizing　enzyme　and　involving　simple　solvent　extraction　steps,　is　developed　to　yield　an　extract　with　high　content　of　steryl　glucosides　(SG)　and　acyl　steryl　glucosides　(ASG)　from　soy　lecithin　-　the　by-product　of　vegetable　oil　refining.　Phospholipase　A(1)　is　used　to　convert　phospholipids　in　soy　lecithin　into　more　hydrophilic　hydrolysates,　from　which　SG　and　ASG　are　separated　by　solvent　extractions.　A　3x3　full　factorial　design　is　employed　to　investigate　the　effects　of　two　parameters　(enzyme　dose　and　reaction　time)　on　three　responses　(yield　of　extract,　SG/ASG　content　of　extract,　and　recovery　of　SG/ASG).　There　are　significant　enzyme　dose　-　reaction　time　interaction　effects　on　all　the　responses,　except　for　yield　of　extract.　The　highest　SG　(including　ASG)　content　of　more　than　90%　in　the　extract　is　achieved　at　enzyme　dose　of　0.03g　and　reaction　time　of　16h,　where　the　yield　of　extract　obtained　is　1.43%.Practical　Applications:　Conventionally,　steryl　glucosides　and　acyl　steryl　glucosides　are　isolated　from　lipid　extract　and　purified　by　chromatographic　methods,　which　result　in　a　small　amount　of　high　purity　steryl　glucosides　and　acyl　steryl　glucosides,　and　thus　is　not　economic　to　scale　up.　The　method　revealed　in　this　study　employed　an　enzymatic　reaction　followed　by　simple　solvent　extractions,　and　can　be　easily　scaled　up　to　produce　high　purity　steryl　glucosides　and　acyl　steryl　glucosides,　making　them　more　affordable　for　researchers.　In　addition,　the　method　also　presents　a　new　application　for　soy　lecithin　or　lecithin　of　other　plant　origins,　whose　current　applications　are　mostly　related　to　the　main　component-phospholipids.　In　this　study,　a　novel　method　utilizing　enzyme　and　involving　simple　solvent　extraction　steps　is　developed　to　yield　an　extract　with　high　content　of　steryl　glucosides　(SG)　and　acyl　steryl　glucosides　(ASG)　from　soy　lecithin　-　the　by-product　of　vegetable　oil　refining.